Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   KHW15_RS01755 Genome accession   NZ_CP074410
Coordinates   376168..378387 (-) Length   739 a.a.
NCBI ID   WP_213569140.1    Uniprot ID   -
Organism   Pseudomonas syringae strain U643     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 371168..383387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHW15_RS01735 (KHW15_01735) hslU 371289..372626 (-) 1338 WP_003316641.1 ATP-dependent protease ATPase subunit HslU -
  KHW15_RS01740 (KHW15_01740) hslV 372693..373223 (-) 531 WP_213569139.1 ATP-dependent protease subunit HslV -
  KHW15_RS01745 (KHW15_01745) - 373637..374332 (-) 696 WP_004414617.1 SPOR domain-containing protein -
  KHW15_RS01750 (KHW15_01750) argS 374334..376070 (-) 1737 WP_003422551.1 arginine--tRNA ligase -
  KHW15_RS01755 (KHW15_01755) priA 376168..378387 (-) 2220 WP_213569140.1 primosomal protein N' Machinery gene
  KHW15_RS01760 (KHW15_01760) rpmE 378557..378778 (+) 222 WP_002551648.1 50S ribosomal protein L31 -
  KHW15_RS01765 (KHW15_01765) - 378792..379595 (+) 804 WP_044312620.1 thermonuclease family protein -
  KHW15_RS01770 (KHW15_01770) - 379951..381219 (+) 1269 WP_003316648.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 739 a.a.        Molecular weight: 81799.11 Da        Isoelectric Point: 8.5462

>NTDB_id=564673 KHW15_RS01755 WP_213569140.1 376168..378387(-) (priA) [Pseudomonas syringae strain U643]
MPDAILRLALPSPLRRLFDYRAPAGVSRSALQPGMRLRVPFGRREMIGILVEVVDHSEVPADKLKPAIALLDSEAPLPPA
LFKLCLWTSQYYQHSLGDTLSWALPVLLRQGELAESRQERFWHVAPGASVDDPRIARAPKQREALTTLAQHPHGVAHQLL
SKLMLNKDSLNLLLAKGLVYVEVRSHAPSARHEHWLAQPELPLNTEQRAAYEAIRAGFDSFHAFLLAGVTGSGKTEVYLQ
LIRETLEAGKQALVLIPEINLGPQTLARFEQRFNARIALVHSAVNDRERLDAWLAARDGEADIIIGTRSALFTPMKNPGL
IIIDEEHDGSYKQQEGLRYHARDLALVRARQENIPIVLGSATPSLESLHNAYTGRYGLLRLNERAGGAQQPRFMRLDVKS
RPLDSGISGPMQQAIGQTLAAGQQVLVFLNRRGFAPTLLCHDCGWMSGCQRCDARMTVHQRSGELRCHHCGYVERVPRQC
PSCGKVDLRPVGAGTERAEERLAILFPDYPVLRVDRDSTSRKDAMNQLFTTIQRGQPCILVGTQMLAKGHHFPRVTLVSI
LDADGGLFSGDFRASERMAQLIVQVAGRAGRAEEPGKVIIQTHLADHPLLIQLTEQGYFAFAEQALSERRSAGLPPFSHL
ALLRAEAHKPGQAEAFLDQACSDAEQLVAQMALGGIELLGPVPAPMERRAGRYRAQLLVQSSARAPLHKLLATWLLALEQ
MPSGRQVRWSLDVDPVDLY

Nucleotide


Download         Length: 2220 bp        

>NTDB_id=564673 KHW15_RS01755 WP_213569140.1 376168..378387(-) (priA) [Pseudomonas syringae strain U643]
GTGCCCGACGCCATCCTGCGCCTTGCCCTGCCCTCGCCGCTTCGCCGCCTGTTCGACTACCGCGCCCCAGCGGGCGTGTC
GCGCAGCGCCTTGCAGCCGGGCATGCGTCTGCGAGTGCCGTTCGGACGCCGGGAAATGATCGGCATTCTGGTGGAAGTGG
TCGATCACAGCGAAGTGCCCGCCGACAAGCTCAAGCCGGCGATCGCCCTGCTCGACAGCGAAGCGCCGCTGCCACCTGCG
CTGTTCAAGCTGTGCCTGTGGACCTCGCAGTACTACCAGCACAGCCTCGGAGACACACTGAGCTGGGCCTTGCCGGTGCT
GTTGCGTCAGGGCGAACTGGCCGAAAGTCGCCAGGAACGCTTCTGGCACGTGGCCCCGGGCGCCAGCGTCGACGATCCGC
GCATCGCCCGCGCGCCCAAGCAGCGCGAGGCCCTGACCACTCTTGCCCAGCACCCGCACGGGGTGGCGCATCAACTGCTA
AGCAAGCTGATGCTTAACAAGGACAGCCTCAACCTGTTGCTGGCCAAGGGGCTGGTGTACGTCGAGGTCCGCAGCCATGC
GCCCAGCGCACGCCATGAACACTGGCTGGCCCAGCCTGAATTGCCGCTCAACACCGAACAACGCGCCGCTTACGAAGCGA
TCCGCGCCGGGTTCGACAGCTTTCATGCTTTTCTGCTGGCCGGCGTCACCGGCAGCGGCAAGACTGAAGTCTATCTGCAA
CTGATCCGCGAAACCCTCGAAGCCGGCAAGCAGGCGCTGGTGCTGATCCCGGAAATCAACCTCGGCCCGCAGACCCTGGC
GCGCTTCGAGCAACGCTTCAATGCGCGCATTGCGCTGGTCCACTCGGCGGTCAACGACCGCGAACGGCTGGATGCCTGGC
TGGCAGCTCGCGATGGCGAGGCGGACATCATCATCGGCACGCGTTCGGCGCTGTTCACGCCGATGAAAAACCCCGGCCTG
ATCATCATCGACGAAGAACACGACGGCTCCTATAAACAGCAGGAAGGTCTGCGCTATCACGCCCGCGATCTGGCCTTGGT
GCGGGCGCGTCAGGAAAACATTCCGATTGTCCTTGGCTCGGCCACACCGTCCCTGGAAAGCCTGCACAACGCTTACACCG
GCCGCTACGGTCTGCTGCGCCTGAATGAGCGCGCGGGCGGCGCACAGCAGCCGCGTTTCATGCGCCTGGACGTCAAAAGC
CGACCTCTGGACAGCGGCATCTCCGGGCCCATGCAGCAGGCCATCGGCCAGACGCTCGCGGCAGGCCAGCAGGTCCTGGT
GTTTCTCAACCGTCGCGGCTTTGCGCCAACCTTGCTGTGCCACGACTGCGGCTGGATGTCCGGCTGCCAACGCTGCGATG
CACGCATGACCGTGCACCAGCGCTCGGGCGAGCTGCGCTGCCATCACTGCGGGTATGTCGAGCGCGTACCGCGTCAGTGC
CCGTCGTGCGGCAAGGTGGATTTGCGCCCGGTAGGCGCCGGCACCGAGCGCGCCGAAGAGCGCCTGGCGATTCTGTTTCC
GGACTACCCCGTACTGCGTGTCGACCGCGACAGCACGTCGCGCAAGGACGCCATGAACCAGCTGTTCACGACGATTCAGC
GCGGTCAGCCGTGCATTCTGGTCGGCACGCAAATGCTCGCCAAGGGCCATCACTTTCCCCGGGTCACGCTGGTCTCGATT
CTGGACGCCGACGGCGGGCTGTTTTCCGGCGACTTCCGCGCCAGCGAGCGCATGGCGCAATTAATCGTGCAGGTGGCAGG
TCGTGCCGGGCGCGCCGAAGAGCCTGGCAAGGTGATCATCCAGACCCACCTGGCCGACCACCCACTGCTGATCCAGCTGA
CCGAGCAGGGTTATTTCGCCTTTGCCGAACAGGCCTTGAGCGAGCGCCGTTCGGCGGGTCTGCCACCGTTTTCACACCTC
GCACTGCTGCGCGCTGAAGCGCATAAGCCGGGACAGGCCGAGGCCTTTCTCGATCAGGCCTGCAGCGATGCGGAGCAACT
AGTCGCGCAGATGGCGCTGGGCGGCATCGAGCTGCTGGGCCCGGTTCCGGCACCGATGGAACGCCGCGCCGGGCGTTATC
GTGCGCAACTGCTGGTGCAGTCCAGCGCCCGCGCACCCTTGCATAAATTGCTTGCCACCTGGCTGCTCGCGCTGGAGCAA
ATGCCCAGCGGTCGGCAGGTCAGATGGTCGCTGGATGTGGATCCTGTGGATCTTTATTAA

Domains

Predicted by InterproScan.

Primosomal protein N C-terminal domain (PriA_C)

(636-735)

DEAD/DEAH box helicase (DEAD)

(207-367)

3DNA-binding domain (3BD) (PriA_3primeBD)

(9-105)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(449-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

47.844

100

0.48