Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   KHA81_RS18065 Genome accession   NZ_CP074391
Coordinates   3650491..3651636 (+) Length   381 a.a.
NCBI ID   WP_015387560.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain L-17     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 3645491..3656636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHA81_RS18045 - 3645965..3646681 (-) 717 WP_014306017.1 Crp/Fnr family transcriptional regulator -
  KHA81_RS18050 narK 3646779..3647969 (-) 1191 WP_014306018.1 nitrate transporter NarK -
  KHA81_RS18055 argS 3648104..3649774 (-) 1671 WP_003151071.1 arginine--tRNA ligase -
  KHA81_RS18060 - 3649771..3650199 (-) 429 WP_003151069.1 DUF1934 family protein -
  KHA81_RS18065 rapF 3650491..3651636 (+) 1146 WP_015387560.1 tetratricopeptide repeat protein Regulator
  KHA81_RS18070 - 3651620..3651739 (+) 120 WP_015387559.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  KHA81_RS18075 - 3651938..3652141 (+) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  KHA81_RS18080 - 3652155..3652358 (+) 204 WP_003151062.1 hypothetical protein -
  KHA81_RS19805 - 3652393..3652579 (+) 187 Protein_3525 DUF4177 domain-containing protein -
  KHA81_RS18085 - 3652767..3653048 (+) 282 WP_014306021.1 hypothetical protein -
  KHA81_RS18090 - 3653092..3653604 (+) 513 WP_015387558.1 stage II sporulation protein M -
  KHA81_RS18095 - 3654195..3654413 (-) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  KHA81_RS18100 speB 3654583..3655455 (-) 873 WP_003151054.1 agmatinase -
  KHA81_RS18105 speE 3655515..3656345 (-) 831 WP_003151052.1 spermidine synthase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45541.70 Da        Isoelectric Point: 4.6084

>NTDB_id=564590 KHA81_RS18065 WP_015387560.1 3650491..3651636(+) (rapF) [Bacillus amyloliquefaciens strain L-17]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEEDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQGNYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNMAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKMMYADLEDFALDVAKYYHERENFEKAAAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=564590 KHA81_RS18065 WP_015387560.1 3650491..3651636(+) (rapF) [Bacillus amyloliquefaciens strain L-17]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTGCGGAGAGAAATCAAAGAAGAGCTTGCAGGCCTGGAAGAAGATCAGGATCTTCATCTGTACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGGCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTGGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAGCAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCTTT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAGGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGGAAATTACAAACCA
GCCATTGATTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATTATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATATGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAATATGAATTTTTAGAATCTTTGTATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAAATGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAGAATTTCGAGAAGGCTGCTGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(189-253)

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.74

100

0.787
  rapC Bacillus subtilis subsp. subtilis str. 168

57.105

99.738

0.57