Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KHA81_RS02035 Genome accession   NZ_CP074391
Coordinates   376490..376927 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain L-17     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 371490..381927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHA81_RS19500 - 372070..372195 (-) 126 Protein_356 hypothetical protein -
  KHA81_RS02000 - 372348..373550 (+) 1203 WP_015388720.1 GTP-binding protein -
  KHA81_RS02005 - 373615..374751 (+) 1137 WP_003156594.1 zinc-dependent alcohol dehydrogenase -
  KHA81_RS02010 - 374766..375200 (+) 435 WP_003156592.1 RDD family protein -
  KHA81_RS02015 - 375272..375592 (+) 321 WP_015388719.1 YckD family protein -
  KHA81_RS02020 - 375696..375812 (+) 117 WP_015388718.1 family 1 glycosylhydrolase -
  KHA81_RS02025 - 375811..376029 (+) 219 Protein_362 family 1 glycosylhydrolase -
  KHA81_RS02030 nin/comJ 376070..376468 (-) 399 WP_003156589.1 competence protein ComJ Regulator
  KHA81_RS02035 nucA/comI 376490..376927 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  KHA81_RS02040 hxlB 377286..377843 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  KHA81_RS02045 hxlA 377840..378475 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  KHA81_RS02050 - 378707..379069 (+) 363 WP_003156585.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=564526 KHA81_RS02035 WP_003156588.1 376490..376927(-) (nucA/comI) [Bacillus amyloliquefaciens strain L-17]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=564526 KHA81_RS02035 WP_003156588.1 376490..376927(-) (nucA/comI) [Bacillus amyloliquefaciens strain L-17]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641