Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KG086_RS10300 Genome accession   NZ_CP074378
Coordinates   2192245..2193609 (-) Length   454 a.a.
NCBI ID   WP_129302545.1    Uniprot ID   A0A4Q1TNC3
Organism   Lacticaseibacillus chiayiensis strain FBL7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2187245..2198609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KG086_RS10280 (KG086_10280) - 2188153..2188929 (-) 777 WP_213224076.1 ABC transporter permease -
  KG086_RS10285 (KG086_10285) - 2188955..2189833 (-) 879 WP_129302542.1 ATP-binding cassette domain-containing protein -
  KG086_RS10290 (KG086_10290) - 2190113..2191009 (+) 897 WP_129302543.1 helix-turn-helix domain-containing protein -
  KG086_RS10295 (KG086_10295) - 2191105..2192220 (-) 1116 WP_129302544.1 PIN/TRAM domain-containing protein -
  KG086_RS10300 (KG086_10300) radA 2192245..2193609 (-) 1365 WP_129302545.1 DNA repair protein RadA Machinery gene
  KG086_RS10305 (KG086_10305) - 2193629..2194168 (-) 540 WP_129302546.1 dUTP diphosphatase -
  KG086_RS10310 (KG086_10310) - 2194432..2194722 (+) 291 WP_129302555.1 GNAT family N-acetyltransferase -
  KG086_RS10315 (KG086_10315) - 2195094..2196413 (+) 1320 WP_129302547.1 aminopeptidase C -
  KG086_RS10320 (KG086_10320) - 2196543..2197889 (+) 1347 WP_129302548.1 C1 family peptidase -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49027.49 Da        Isoelectric Point: 8.0456

>NTDB_id=564475 KG086_RS10300 WP_129302545.1 2192245..2193609(-) (radA) [Lacticaseibacillus chiayiensis strain FBL7]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSTKSAPRQTRSGSKVKPMRMNEVTVAKETRVKTGLAELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLASTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMLSIEAVI
DQMQPDYVVIDSVQTMNVPDMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQNGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYSDKEIPPTDCFVGEIGLTGEV
RRVNRIEDRVKEAAKLGFKRIFVPRNNLQGWHAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=564475 KG086_RS10300 WP_129302545.1 2192245..2193609(-) (radA) [Lacticaseibacillus chiayiensis strain FBL7]
ATGGCGAAAGCAAAAACACAGTACGTATGTCAAAATTGTGGTTATATTTCAGCCACTTATTTAGGGCGTTGTCCCAATTG
TGGCGGTTGGAACACTTTAGTCGAAGAAACCGTCAGTTCAACCAAATCAGCACCGCGGCAGACACGGTCAGGCAGTAAAG
TCAAACCGATGCGGATGAATGAGGTAACAGTTGCCAAGGAAACGCGGGTTAAAACCGGGTTGGCAGAATTAAACCGGGTG
CTTGGCGGCGGCGTGGTGCCGGGGTCGCTTGTTTTGATTGGCGGCGATCCGGGTATTGGCAAATCAACCTTGTTGCTGCA
GGTGTCTGGGCAGCTAGCGAGCACTGGAGGTACCGTATTATACGTTTCTGGCGAAGAAAGTGCCAGTCAGATCAAAATGC
GGGCTGGGCGGTTGGGCGTTGCTAATTCGGGGATGTATCTGTATCCGGAAACCGACATGCTTAGTATCGAAGCCGTCATT
GATCAGATGCAACCGGATTATGTTGTCATCGACTCGGTTCAAACCATGAATGTGCCGGATATGAACTCTGCAGTTGGTTC
GGTTGCCCAGATTCGTGAGGTTACCGCTGAATTGATGCGCATTGCCAAGTCAAAAGGCGTGACCATTTTCATTGTCGGGC
ACGTGACCAAGGAAGGCGCGATCGCCGGGCCTAAAATTCTTGAGCATATGGTCGACACCGTGCTTTATTTTGAAGGCGAT
ATGCACCACACATACCGGATTTTACGGTCAGTGAAAAATCGCTTTGGCTCAACTAATGAAATCGGCATTTTCGAGATGCA
TCAAAATGGTTTGCAGGAGGTCGCCAATCCGTCTGAAATCTTTTTGGAAGAACGCCTAGCCGGGGCGACCGGTTCGGCGG
TGGTGGTTTCGATGGAAGGCACGCGGCCGATTTTGGTTGAAATTCAGGCGTTGATCAGTCCTACCATGTATGGCAATGCC
AAGCGCACGAGCAGCGGACTGGATCACAATCGGGTCAGCTTAATTATGGCGGTGCTGGAAAAACGCGCCAATCTTATGCT
GCAGAATCAAGATGCTTACCTGAAGGCCACTGGTGGCGTTAAGTTGGATGAGCCGGCAATTGACTTAGCGATGGCCGTTG
CGATTGCCTCGTCCTACAGCGATAAAGAAATTCCGCCGACTGATTGTTTTGTCGGTGAAATCGGCTTAACCGGTGAAGTG
CGGCGGGTCAACCGCATTGAAGACCGGGTCAAAGAGGCGGCCAAGCTGGGATTTAAGCGGATTTTTGTTCCGCGCAACAA
CTTGCAAGGCTGGCATGCTCCCAAAGATATTCAAGTGATTGGGGTCACAAGCATTGCCGAAGCGTTGCATAAGGTTTTTA
ATTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(69-219)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Q1TNC3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

68.653

99.78

0.685
  radA Streptococcus pneumoniae D39

68.653

99.78

0.685
  radA Streptococcus pneumoniae R6

68.653

99.78

0.685
  radA Streptococcus pneumoniae TIGR4

68.653

99.78

0.685
  radA Streptococcus mitis NCTC 12261

68.433

99.78

0.683
  radA Streptococcus mitis SK321

68.433

99.78

0.683
  radA Bacillus subtilis subsp. subtilis str. 168

64.693

100

0.65