Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HLG70_RS21115 Genome accession   NZ_CP074375
Coordinates   4682283..4683728 (-) Length   481 a.a.
NCBI ID   WP_171666250.1    Uniprot ID   -
Organism   Achromobacter deleyi strain As-55     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4677283..4688728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HLG70_RS21090 (HLG70_21085) - 4678328..4679014 (+) 687 WP_171666256.1 hypothetical protein -
  HLG70_RS21095 (HLG70_21090) bfr 4679098..4679574 (-) 477 WP_013390908.1 bacterioferritin -
  HLG70_RS21100 (HLG70_21095) - 4679944..4680858 (-) 915 WP_171666254.1 DMT family transporter -
  HLG70_RS21105 (HLG70_21100) - 4680992..4681849 (+) 858 WP_171666252.1 LysR substrate-binding domain-containing protein -
  HLG70_RS21110 (HLG70_21105) - 4681880..4682161 (-) 282 WP_057286111.1 (2Fe-2S)-binding protein -
  HLG70_RS21115 (HLG70_21110) rpoN/rpoN1 4682283..4683728 (-) 1446 WP_171666250.1 RNA polymerase factor sigma-54 Machinery gene
  HLG70_RS21120 (HLG70_21115) - 4683727..4684359 (+) 633 WP_171666248.1 hypothetical protein -
  HLG70_RS21125 (HLG70_21120) leuA 4684915..4686627 (+) 1713 WP_171666246.1 2-isopropylmalate synthase -
  HLG70_RS21130 (HLG70_21125) - 4686730..4688058 (-) 1329 WP_171666244.1 amidase -

Sequence

Protein


Download         Length: 481 a.a.        Molecular weight: 52665.96 Da        Isoelectric Point: 4.8072

>NTDB_id=564416 HLG70_RS21115 WP_171666250.1 4682283..4683728(-) (rpoN/rpoN1) [Achromobacter deleyi strain As-55]
MQNIVPMTRPILELRPGQHLTLTPQLQQSIRLLQLSTLELEAEISQVLAENPLLDREDESPSAEAQAESERESSVQDDEP
AAERETPVDEMPGSGGVYPDDDSSLPEAARPDTLREYLLGQLALTRAAPRDVALASLLIDELDDNGYLGSPLEEILGWLP
AELEPDIDELRAALSLLQSFDPVGIGARDMAECLQLQLRNPDPARLPEASDAAVLACARRICAQHLPLLATGNSARLCAA
VGCDEATFRAAHSLILRLEPRPGRAWTVPAAEYAVPDVIVRKTRRGWQVTLNSAAVPRLQINGLYAQMLGNQKESAHAGL
QAQLQQARWMIRNVEQRFDTILRVSQAIVERQTAFFSQGPAAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTLEL
KRFFGAGVSTEGGDATSATAVQTYIRQMVADENRAKPLSDSQIMQKLADQGIVIARRTVAKYREALRIAPAALRKAQASM
R

Nucleotide


Download         Length: 1446 bp        

>NTDB_id=564416 HLG70_RS21115 WP_171666250.1 4682283..4683728(-) (rpoN/rpoN1) [Achromobacter deleyi strain As-55]
ATGCAAAATATTGTACCTATGACCCGCCCAATTTTAGAACTGCGACCTGGTCAGCACCTGACGCTGACCCCACAATTGCA
GCAATCGATACGATTGTTGCAATTGTCCACGCTCGAGCTCGAGGCCGAGATCTCGCAAGTGCTTGCGGAGAATCCGCTGC
TGGATCGGGAAGACGAGTCGCCGTCGGCCGAGGCGCAAGCCGAATCCGAGCGTGAATCCTCCGTCCAGGACGACGAGCCT
GCGGCCGAGCGTGAGACGCCGGTTGACGAGATGCCGGGGTCTGGCGGGGTGTATCCCGATGACGACTCCAGCCTGCCCGA
GGCGGCCCGGCCGGATACCTTGCGCGAATACCTGCTCGGACAGCTTGCCCTGACGCGCGCGGCGCCGCGCGACGTCGCGC
TCGCGTCGCTGCTGATCGACGAATTGGACGACAACGGTTACCTGGGCTCTCCGCTTGAGGAGATCCTGGGCTGGCTTCCT
GCCGAACTCGAGCCGGATATCGACGAATTGCGCGCGGCGCTATCCCTGTTGCAGTCATTTGATCCGGTCGGCATCGGCGC
GCGGGACATGGCCGAGTGCCTGCAGCTGCAGTTGCGCAATCCGGACCCCGCCCGTCTGCCCGAGGCGTCGGATGCCGCCG
TGCTGGCCTGTGCCAGGCGGATTTGCGCCCAGCACCTGCCGCTGCTGGCCACGGGGAATTCCGCGCGCCTGTGCGCGGCG
GTGGGCTGCGACGAGGCCACGTTCCGTGCGGCGCACTCCCTGATTCTGCGTCTGGAGCCGCGTCCGGGGCGGGCCTGGAC
CGTCCCGGCGGCCGAGTACGCGGTGCCGGACGTCATCGTGCGCAAGACCCGGCGCGGCTGGCAGGTCACGCTCAACAGCG
CCGCGGTGCCCCGCCTGCAGATCAATGGGCTGTATGCCCAGATGCTGGGCAACCAGAAGGAGTCCGCGCATGCGGGCCTG
CAGGCCCAGTTGCAACAGGCCAGATGGATGATCCGCAACGTCGAGCAGCGCTTCGACACCATCCTCCGGGTGTCCCAGGC
CATCGTCGAACGCCAGACGGCCTTTTTCAGCCAAGGGCCCGCCGCCATGCGGCCGCTGATCCTGAAGGACATCGCTGGCG
AGCTGGGTTTGCATGAATCCACCATTTCACGCGCCACAACGCAGAAGTACATGCTCACTCCGTTTGGCACGTTGGAACTC
AAGCGGTTCTTTGGCGCCGGCGTTTCGACGGAAGGCGGGGACGCCACCTCAGCCACCGCGGTGCAGACCTACATCCGCCA
GATGGTGGCCGACGAGAACCGGGCCAAGCCCTTGTCCGACAGCCAGATCATGCAAAAACTGGCCGATCAGGGGATAGTGA
TCGCCCGGCGGACGGTGGCAAAGTACCGCGAAGCGCTGCGAATTGCCCCCGCGGCGCTGCGCAAGGCCCAGGCTTCGATG
CGGTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54, DNA binding domain (Sigma54_DBD)

(320-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(108-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

50.298

100

0.526