Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   KHA74_RS12725 Genome accession   NZ_CP074348
Coordinates   2625922..2626959 (-) Length   345 a.a.
NCBI ID   WP_015417819.1    Uniprot ID   -
Organism   Bacillus velezensis strain SW5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2620922..2631959
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHA74_RS12675 (KHA74_12585) - 2621156..2621941 (-) 786 WP_007408329.1 TasA family protein -
  KHA74_RS12680 (KHA74_12590) - 2622006..2622590 (-) 585 WP_015240205.1 signal peptidase I -
  KHA74_RS12685 (KHA74_12595) tapA 2622562..2623233 (-) 672 WP_015417813.1 amyloid fiber anchoring/assembly protein TapA -
  KHA74_RS12690 (KHA74_12600) - 2623492..2623821 (+) 330 WP_094032245.1 DUF3889 domain-containing protein -
  KHA74_RS12695 (KHA74_12605) - 2623861..2624040 (-) 180 WP_003153093.1 YqzE family protein -
  KHA74_RS12700 (KHA74_12610) comGG 2624097..2624474 (-) 378 WP_015417814.1 competence type IV pilus minor pilin ComGG Machinery gene
  KHA74_RS12705 (KHA74_12615) comGF 2624475..2624975 (-) 501 WP_254922226.1 competence type IV pilus minor pilin ComGF -
  KHA74_RS12710 (KHA74_12620) comGE 2624884..2625198 (-) 315 WP_094032244.1 competence type IV pilus minor pilin ComGE -
  KHA74_RS12715 (KHA74_12625) comGD 2625182..2625619 (-) 438 WP_094032243.1 competence type IV pilus minor pilin ComGD Machinery gene
  KHA74_RS12720 (KHA74_12630) comGC 2625609..2625917 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  KHA74_RS12725 (KHA74_12635) comGB 2625922..2626959 (-) 1038 WP_015417819.1 competence type IV pilus assembly protein ComGB Machinery gene
  KHA74_RS12730 (KHA74_12640) comGA 2626946..2628016 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  KHA74_RS12735 (KHA74_12645) - 2628209..2629159 (-) 951 WP_015417820.1 magnesium transporter CorA family protein -
  KHA74_RS12740 (KHA74_12650) - 2629305..2630606 (+) 1302 WP_012117986.1 hemolysin family protein -
  KHA74_RS12745 (KHA74_12655) - 2630644..2631474 (-) 831 WP_039254482.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39689.06 Da        Isoelectric Point: 9.5935

>NTDB_id=564078 KHA74_RS12725 WP_015417819.1 2625922..2626959(-) (comGB) [Bacillus velezensis strain SW5]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLQLQLHKRQLAELKEGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLIFSVCVMFYILQSLIIPQFSGIYQSMNMNTSGTTAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=564078 KHA74_RS12725 WP_015417819.1 2625922..2626959(-) (comGB) [Bacillus velezensis strain SW5]
ATGAAGCGGATTAAACGAACATGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGCCTGATTGAAGGGCTCAGACTGCTGCAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGAAGGAAGGAATCC
GCCGGCTGAGAGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTTTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACGTCCGGTACGACTGCTTTCATCTTTGCGTTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTTTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAGTCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATACCCCTCTTGGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGAGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
TGGGCATCCGTGTTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.635

93.623

0.614