Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   KHA74_RS12480 Genome accession   NZ_CP074348
Coordinates   2586602..2587402 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain SW5     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2581602..2592402
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHA74_RS12450 (KHA74_12360) - 2582142..2582774 (+) 633 WP_007408365.1 helix-turn-helix domain-containing protein -
  KHA74_RS12455 (KHA74_12365) - 2583071..2583208 (+) 138 WP_014470646.1 hypothetical protein -
  KHA74_RS12460 (KHA74_12370) - 2583302..2584420 (+) 1119 WP_061861489.1 NADH-dependent flavin oxidoreductase -
  KHA74_RS12465 (KHA74_12375) - 2584546..2584758 (+) 213 WP_007408362.1 spore germination protein -
  KHA74_RS12470 (KHA74_12380) - 2584884..2585096 (+) 213 WP_003153181.1 spore germination protein -
  KHA74_RS12475 (KHA74_12385) alr 2585272..2586453 (+) 1182 WP_012117960.1 alanine racemase -
  KHA74_RS12480 (KHA74_12390) spo0A 2586602..2587402 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  KHA74_RS12485 (KHA74_12395) spoIVB 2587683..2588963 (-) 1281 WP_094032247.1 SpoIVB peptidase -
  KHA74_RS12490 (KHA74_12400) recN 2589127..2590857 (-) 1731 WP_015417799.1 DNA repair protein RecN Machinery gene
  KHA74_RS12495 (KHA74_12405) argR 2590889..2591338 (-) 450 WP_003153173.1 transcriptional regulator ArgR -
  KHA74_RS12500 (KHA74_12410) - 2591435..2592280 (-) 846 WP_007612503.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=564071 KHA74_RS12480 WP_003153177.1 2586602..2587402(-) (spo0A) [Bacillus velezensis strain SW5]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=564071 KHA74_RS12480 WP_003153177.1 2586602..2587402(-) (spo0A) [Bacillus velezensis strain SW5]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATTGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCAGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATCCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCTGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACTGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAGCCAACCAATTCAGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97