Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L8E65_RS01910 Genome accession   NZ_CP091880
Coordinates   414646..416004 (+) Length   452 a.a.
NCBI ID   WP_014602384.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain US449     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 409646..421004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L8E65_RS01890 (L8E65_01890) - 410483..411049 (-) 567 WP_003110405.1 hypothetical protein -
  L8E65_RS01895 (L8E65_01895) rsmD 411052..411648 (-) 597 WP_023114755.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  L8E65_RS01900 (L8E65_01900) - 411648..413135 (-) 1488 WP_003459246.1 pitrilysin family protein -
  L8E65_RS01905 (L8E65_01905) - 413128..414525 (-) 1398 WP_003084498.1 pitrilysin family protein -
  L8E65_RS01910 (L8E65_01910) pilA 414646..416004 (+) 1359 WP_014602384.1 signal recognition particle-docking protein FtsY Machinery gene
  L8E65_RS01915 (L8E65_01915) ftsE 416001..416672 (+) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  L8E65_RS01920 (L8E65_01920) ftsX 416672..417679 (+) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  L8E65_RS01925 (L8E65_01925) rpoH 417793..418647 (+) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  L8E65_RS01930 (L8E65_01930) - 418712..419317 (+) 606 WP_004346634.1 hypothetical protein -
  L8E65_RS01935 (L8E65_01935) mtgA 419322..420053 (-) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  L8E65_RS01940 (L8E65_01940) - 420090..420467 (+) 378 WP_003084513.1 DUF423 domain-containing protein -
  L8E65_RS01945 (L8E65_01945) thiS 420570..420770 (+) 201 WP_003084514.1 sulfur carrier protein ThiS -

Sequence

Protein


Download         Length: 452 a.a.        Molecular weight: 48098.50 Da        Isoelectric Point: 4.5525

>NTDB_id=563950 L8E65_RS01910 WP_014602384.1 414646..416004(+) (pilA) [Pseudomonas aeruginosa strain US449]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPAPV
AEAPLASDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTSAS
IGEGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPLQV
DVAREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIFDA
VQAAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLALTK
LDGTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1359 bp        

>NTDB_id=563950 L8E65_RS01910 WP_014602384.1 414646..416004(+) (pilA) [Pseudomonas aeruginosa strain US449]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGGCGCCGGTT
GCCGAAGCACCGCTCGCCAGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCAACC
CGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCGCTGGCCGCCG
CTCCGGCAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGGCTGTCCAAGACCAGCGCCAGC
ATTGGCGAAGGCATGGCCAGCCTGTTCCTCGGTCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGACGCGCCT
GCTGACCGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGGAGCTGG
CCGACAGCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTGCAGGTC
GACGTCGCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAAGCTGGC
GAAGAAGCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGCAGTTGC
AGGTCTGGGGCGAGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTCGACGCG
GTGCAGGCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAACCTGAT
GGAGGAGCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGCTCGACG
CCGGCACCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTGGCCCTGACCAAA
CTGGACGGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGGTGTCGG
CGAGGGGATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACGCATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(162-227)

SRP54-type protein, GTPase domain (SRP54)

(247-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.235

96.018

0.454