Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KGP25_RS21850 Genome accession   NZ_CP074170
Coordinates   4499561..4500994 (+) Length   477 a.a.
NCBI ID   WP_045910109.1    Uniprot ID   -
Organism   Enterobacter sp. JBIWA003     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4494561..4505994
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KGP25_RS21820 (KGP25_21690) - 4495128..4496105 (+) 978 WP_045334591.1 calcium/sodium antiporter -
  KGP25_RS21825 (KGP25_21695) kdsD 4496119..4497105 (+) 987 WP_008501450.1 arabinose-5-phosphate isomerase KdsD -
  KGP25_RS21830 (KGP25_21700) kdsC 4497120..4497686 (+) 567 WP_008501449.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  KGP25_RS21835 (KGP25_21705) lptC 4497683..4498258 (+) 576 WP_024906353.1 LPS export ABC transporter periplasmic protein LptC -
  KGP25_RS21840 (KGP25_21710) lptA 4498227..4498781 (+) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  KGP25_RS21845 (KGP25_21715) lptB 4498788..4499513 (+) 726 WP_008501446.1 LPS export ABC transporter ATP-binding protein -
  KGP25_RS21850 (KGP25_21720) rpoN/rpoN1 4499561..4500994 (+) 1434 WP_045910109.1 RNA polymerase factor sigma-54 Machinery gene
  KGP25_RS21855 (KGP25_21725) hpf 4501017..4501304 (+) 288 WP_013098943.1 ribosome hibernation promoting factor -
  KGP25_RS21860 (KGP25_21730) ptsN 4501387..4501878 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  KGP25_RS21865 (KGP25_21735) rapZ 4501924..4502778 (+) 855 WP_045910110.1 RNase adapter RapZ -
  KGP25_RS21870 (KGP25_21740) npr 4502775..4503047 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  KGP25_RS21875 (KGP25_21745) mtgA 4503089..4503814 (-) 726 WP_048225123.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  KGP25_RS21880 (KGP25_21750) elbB 4503811..4504464 (-) 654 WP_048029292.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54116.87 Da        Isoelectric Point: 4.3080

>NTDB_id=563759 KGP25_RS21850 WP_045910109.1 4499561..4500994(+) (rpoN/rpoN1) [Enterobacter sp. JBIWA003]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWLDEARVIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKNNGRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=563759 KGP25_RS21850 WP_045910109.1 4499561..4500994(+) (rpoN/rpoN1) [Enterobacter sp. JBIWA003]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACCCAGCAATCGCAGGATACAGAAGCGCTCGATACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGCACCCCTTCCGGTACGCGTGCAGACTACCAGGACGA
TGAGCTACCGGTCTATCAGGGAGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACGTCAATTGTCGATGCCGTTGACGACACTGGCTATTTGACCGTCACGCTGGACGAT
ATTCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTTGAAGCTGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTAGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCGTGATCATCAGCGATCATCTTGATCTGCTGGCGAACCACGATTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAGTACGTGATTCCGGATGTGCTGGTCAGAAAAAATAACGGTCGCTGGGTCGTTGAACTGAACTCTGACAGTA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATTTGCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGCAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGTGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCCACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCGCTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CTCGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48