Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L7H22_RS12935 Genome accession   NZ_CP091839
Coordinates   2672318..2673811 (-) Length   497 a.a.
NCBI ID   WP_238058956.1    Uniprot ID   -
Organism   Escherichia coli strain SWYHX138.261ft     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2667318..2678811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L7H22_RS12910 (L7H22_12910) panM 2667552..2667935 (+) 384 WP_000778768.1 aspartate 1-decarboxylase autocleavage activator PanM -
  L7H22_RS12915 (L7H22_12915) livJ 2668123..2669226 (-) 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  L7H22_RS12920 (L7H22_12920) rpoH 2669497..2670351 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  L7H22_RS12925 (L7H22_12925) ftsX 2670596..2671654 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  L7H22_RS12930 (L7H22_12930) ftsE 2671647..2672315 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  L7H22_RS12935 (L7H22_12935) pilA 2672318..2673811 (-) 1494 WP_238058956.1 signal recognition particle-docking protein FtsY Machinery gene
  L7H22_RS12940 (L7H22_12940) rsmD 2673961..2674557 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  L7H22_RS12945 (L7H22_12945) yhhL 2674547..2674816 (+) 270 WP_001311191.1 DUF1145 family protein -
  L7H22_RS12950 (L7H22_12950) yhhM 2674819..2675178 (-) 360 WP_000042895.1 DUF2500 domain-containing protein -
  L7H22_RS12955 (L7H22_12955) yhhN 2675319..2675945 (+) 627 WP_053898938.1 lysoplasmalogenase -
  L7H22_RS12960 (L7H22_12960) zntA 2676019..2678217 (+) 2199 WP_238058957.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  L7H22_RS12965 (L7H22_12965) tusA 2678319..2678564 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 54512.41 Da        Isoelectric Point: 4.1831

>NTDB_id=563728 L7H22_RS12935 WP_238058956.1 2672318..2673811(-) (pilA) [Escherichia coli strain SWYHX138.261ft]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVKETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETALA
AEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADV
GVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQ
GKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKI
VRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDL
RPFKADDFIEALFARED

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=563728 L7H22_RS12935 WP_238058956.1 2672318..2673811(-) (pilA) [Escherichia coli strain SWYHX138.261ft]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCGGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCCTCTGAACAAGCCGTTGAAG
AGCAGCCGCAAGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAAAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATTACCGACGAAGAGCTGGAAACGGCGCTGGCT
GCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCA
GGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCGGAT
TTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAGGAGCTGGAAGAGCAGCTTTTGATCGCCGATGTG
GGTGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGACGCCGAGGCGCT
CTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGCAAAGCGCCGT
TTGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAGCAG
GGTAAATCGGTGATGCTGGCGGCGGGTGATACTTTCCGTGCAGCTGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCGCAA
CAATATTCCGGTGATTGCCCAGCATACCGGGGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAGCGC
GTAATATCGACGTCCTGATTGCCGATACAGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAAATC
GTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAACGC
GGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGAAAG
GCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGATTTG
CGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(210-275)

SRP54-type protein, GTPase domain (SRP54)

(293-493)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.698

77.264

0.376