Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KGP18_RS02485 Genome accession   NZ_CP074154
Coordinates   514541..515974 (-) Length   477 a.a.
NCBI ID   WP_023309360.1    Uniprot ID   A0A1Z3N130
Organism   Enterobacter asburiae strain JBIWA007     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 509541..520974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KGP18_RS02455 (KGP18_02455) elbB 511073..511726 (+) 654 WP_085841468.1 isoprenoid biosynthesis glyoxalase ElbB -
  KGP18_RS02460 (KGP18_02460) mtgA 511723..512448 (+) 726 WP_223535766.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  KGP18_RS02465 (KGP18_02465) npr 512488..512760 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  KGP18_RS02470 (KGP18_02470) rapZ 512757..513611 (-) 855 WP_014885397.1 RNase adapter RapZ -
  KGP18_RS02475 (KGP18_02475) ptsN 513657..514148 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  KGP18_RS02480 (KGP18_02480) hpf 514231..514518 (-) 288 WP_008501444.1 ribosome hibernation promoting factor -
  KGP18_RS02485 (KGP18_02485) rpoN/rpoN1 514541..515974 (-) 1434 WP_023309360.1 RNA polymerase factor sigma-54 Machinery gene
  KGP18_RS02490 (KGP18_02490) lptB 516022..516747 (-) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  KGP18_RS02495 (KGP18_02495) lptA 516754..517308 (-) 555 WP_023309358.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  KGP18_RS02500 (KGP18_02500) lptC 517277..517852 (-) 576 WP_023309357.1 LPS export ABC transporter periplasmic protein LptC -
  KGP18_RS02505 (KGP18_02505) kdsC 517849..518415 (-) 567 WP_023309356.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  KGP18_RS02510 (KGP18_02510) kdsD 518430..519416 (-) 987 WP_023333606.1 arabinose-5-phosphate isomerase KdsD -
  KGP18_RS02515 (KGP18_02515) - 519430..520407 (-) 978 WP_023309354.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54169.89 Da        Isoelectric Point: 4.3149

>NTDB_id=563679 KGP18_RS02485 WP_023309360.1 514541..515974(-) (rpoN/rpoN1) [Enterobacter asburiae strain JBIWA007]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFSKETPWIDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=563679 KGP18_RS02485 WP_023309360.1 514541..515974(-) (rpoN/rpoN1) [Enterobacter asburiae strain JBIWA007]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
GTTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACTCAGCAGTCACAGGATACAGAAGCGCTCGATACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCCAGCTGGGATGAAATCTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTACCAGGGAGAGACCACCCAGTCGCTACAGGATTACCTGATGTGGCAGGTGGAACTCACCCCCTTCT
CCGATACCGACCGCGCGATTGCCACCTCAATTGTCGATGCAGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATTCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTCGAAGCCGTTCTGAAGCGCATCCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAAAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCATGGATCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCTCCCTGATGCGCGTCACGCGG
CTGAAGGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAGTACGTCATCCCGGACGTGCTGGTCAGAAAACACAATGACCGCTGGGTCGTTGAACTGAATTCAGACAGCA
TCCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGC
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTACTGGCGGATATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATATTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACCGAGGGCGGCGGCGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Z3N130

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48