Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L7H17_RS18400 Genome accession   NZ_CP091836
Coordinates   3828709..3829149 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain SWYHX138.33gt     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3823709..3834149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L7H17_RS18375 (L7H17_18375) coaE 3824199..3824819 (-) 621 WP_238059049.1 dephospho-CoA kinase -
  L7H17_RS18380 (L7H17_18380) - 3824844..3824888 (+) 45 WP_120795372.1 protein YacM -
  L7H17_RS18385 (L7H17_18385) guaC 3825044..3826087 (+) 1044 WP_053889342.1 GMP reductase -
  L7H17_RS18390 (L7H17_18390) hofC 3826122..3827324 (-) 1203 WP_171461493.1 protein transport protein HofC -
  L7H17_RS18395 (L7H17_18395) pilB 3827314..3828699 (-) 1386 WP_171461494.1 type II secretion system protein GspE Machinery gene
  L7H17_RS18400 (L7H17_18400) pilA 3828709..3829149 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  L7H17_RS18405 (L7H17_18405) nadC 3829352..3830245 (-) 894 WP_001135165.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L7H17_RS18410 (L7H17_18410) ampD 3830333..3830884 (+) 552 WP_000923722.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L7H17_RS18415 (L7H17_18415) ampE 3830881..3831735 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  L7H17_RS18420 (L7H17_18420) aroP 3831778..3833151 (-) 1374 WP_000969912.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=563675 L7H17_RS18400 WP_000360904.1 3828709..3829149(-) (pilA) [Escherichia coli strain SWYHX138.33gt]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=563675 L7H17_RS18400 WP_000360904.1 3828709..3829149(-) (pilA) [Escherichia coli strain SWYHX138.33gt]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363