Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KGP24_RS20865 Genome accession   NZ_CP074149
Coordinates   4297884..4299317 (+) Length   477 a.a.
NCBI ID   WP_223561679.1    Uniprot ID   -
Organism   Enterobacter sp. JBIWA008     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4292884..4304317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KGP24_RS20835 (KGP24_20720) - 4293451..4294428 (+) 978 WP_223561676.1 calcium/sodium antiporter -
  KGP24_RS20840 (KGP24_20725) kdsD 4294442..4295428 (+) 987 WP_223561677.1 arabinose-5-phosphate isomerase KdsD -
  KGP24_RS20845 (KGP24_20730) kdsC 4295443..4296009 (+) 567 WP_223563539.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  KGP24_RS20850 (KGP24_20735) lptC 4296006..4296581 (+) 576 WP_223561678.1 LPS export ABC transporter periplasmic protein LptC -
  KGP24_RS20855 (KGP24_20740) lptA 4296550..4297104 (+) 555 WP_020883526.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  KGP24_RS20860 (KGP24_20745) lptB 4297111..4297836 (+) 726 WP_023309359.1 LPS export ABC transporter ATP-binding protein -
  KGP24_RS20865 (KGP24_20750) rpoN/rpoN1 4297884..4299317 (+) 1434 WP_223561679.1 RNA polymerase factor sigma-54 Machinery gene
  KGP24_RS20870 (KGP24_20755) hpf 4299340..4299627 (+) 288 WP_008501444.1 ribosome hibernation promoting factor -
  KGP24_RS20875 (KGP24_20760) ptsN 4299710..4300201 (+) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  KGP24_RS20880 (KGP24_20765) rapZ 4300247..4301101 (+) 855 WP_223561680.1 RNase adapter RapZ -
  KGP24_RS20885 (KGP24_20770) npr 4301098..4301370 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  KGP24_RS20890 (KGP24_20775) mtgA 4301410..4302135 (-) 726 WP_223561681.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  KGP24_RS20895 (KGP24_20780) elbB 4302132..4302785 (-) 654 WP_223563540.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54183.92 Da        Isoelectric Point: 4.3149

>NTDB_id=563670 KGP24_RS20865 WP_223561679.1 4297884..4299317(+) (rpoN/rpoN1) [Enterobacter sp. JBIWA008]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDDEIELEEVEAVLKRIQRFDPIGVAAKDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRSLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNDRWVVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=563670 KGP24_RS20865 WP_223561679.1 4297884..4299317(+) (rpoN/rpoN1) [Enterobacter sp. JBIWA008]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCGATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACCCAGCAATCTCAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCCGAC
GAGCTACCGCTGGATGCAAGCTGGGATGAAATTTACACCGCCGGAACCCCTTCCGGTACGCGTGCAGACTACCAGGATGA
TGAGCTACCGGTCTATCAGGGCGAAACCACCCAGTCGCTGCAGGATTACCTGATGTGGCAGGTGGAGCTGACTCCCTTCT
CCGATACCGACCGCGCGATTGCCACGTCAATTGTCGATGCCGTTGACGACACCGGCTATTTGACCGTCACGCTGGACGAT
ATTCTGGAAAGCATGGGCGACGACGAGATTGAGCTTGAGGAAGTTGAAGCCGTACTGAAGCGCATCCAGCGTTTCGATCC
GATAGGCGTGGCCGCAAAAGATCTGCGTGACTGCCTGCTGATCCAGCTTTCTCAATTCAGCAAAGAGACGCCATGGCTCG
ACGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCCAACCACGATTTCCGCTCCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAAGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGCCCGGGACAGTCGATCCAGACCAG
CGAGCCCGAATACGTCATTCCGGACGTACTGGTCAGAAAACATAATGACCGCTGGGTCGTTGAACTGAACTCAGACAGCA
TCCCTCGCCTGCAAATCAATCAGCAGTATGCCTCCATGTGCACCAGCGCGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGTCTGGAGAGCCGAAATGATACGCTGCTACGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCTTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAGGCCG
TCGAAATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTTTTCTCCAGCCATGTGAATACCGAGGGCGGCGGTGAAGCGTCGTCAACGGCCATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTAAGCGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGACGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48