Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L6507_RS19745 Genome accession   NZ_CP091756
Coordinates   4055645..4056085 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain LC-1302-2020     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4050645..4061085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6507_RS19725 (L6507_19725) aroP 4051643..4053016 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  L6507_RS19730 (L6507_19730) ampE 4053059..4053913 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  L6507_RS19735 (L6507_19735) ampD 4053910..4054461 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L6507_RS19740 (L6507_19740) nadC 4054549..4055442 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L6507_RS19745 (L6507_19745) pilA 4055645..4056085 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  L6507_RS19750 (L6507_19750) pilB 4056095..4057480 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  L6507_RS19755 (L6507_19755) hofC 4057470..4058672 (+) 1203 WP_000157266.1 protein transport protein HofC -
  L6507_RS19760 (L6507_19760) guaC 4058707..4059750 (-) 1044 WP_001217338.1 GMP reductase -
  L6507_RS19765 (L6507_19765) - 4059906..4059950 (-) 45 WP_120795372.1 protein YacM -
  L6507_RS19770 (L6507_19770) coaE 4059975..4060595 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=563394 L6507_RS19745 WP_000360904.1 4055645..4056085(+) (pilA) [Escherichia coli strain LC-1302-2020]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=563394 L6507_RS19745 WP_000360904.1 4055645..4056085(+) (pilA) [Escherichia coli strain LC-1302-2020]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363