Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   BTF_RS00270 Genome accession   NC_020387
Coordinates   54028..55422 (+) Length   464 a.a.
NCBI ID   WP_012983997.1    Uniprot ID   A0A1S6SF54
Organism   Dehalococcoides mccartyi BTF08     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 49028..60422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BTF_RS00265 (btf_54) - 51162..53636 (+) 2475 WP_012983996.1 ATP-dependent Clp protease ATP-binding subunit -
  BTF_RS00270 (btf_55) radA 54028..55422 (+) 1395 WP_012983997.1 DNA repair protein RadA Machinery gene
  BTF_RS00275 (btf_56) - 55409..56092 (+) 684 WP_015407377.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  BTF_RS00280 (btf_57) ispF 56098..56571 (+) 474 WP_015406720.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  BTF_RS00285 (btf_58) cysS 56575..57948 (+) 1374 WP_015407378.1 cysteine--tRNA ligase -
  BTF_RS00295 (btf_60) - 58511..58819 (-) 309 WP_011308733.1 ferritin family protein -
  BTF_RS00300 (btf_61) - 59008..60153 (-) 1146 WP_015407379.1 NTP transferase domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 49429.78 Da        Isoelectric Point: 6.6399

>NTDB_id=56330 BTF_RS00270 WP_012983997.1 54028..55422(+) (radA) [Dehalococcoides mccartyi BTF08]
MDKSRNVYICSNCGHESLKWLGRCPGCQEWNTLEETTIAAPLGRKNAPARVISPAAELSSLNASDTTRRSLSISEFNRVL
GGGIVPGSLMLLGGEPGIGKSTLLLQVAASVAQSGGKVVYVSGEENPAQIKMRAQRLGISGEGLFLMAETDLNAILAQLS
VLCPSLVVIDSIQTVFLPELEAAPGAINQVRESALRLMQWAKSSGASVFITAHVTKEGNIAGPRILEHIVDVVMYFEGES
QSAYRLIRSVKNRFGSTNEVGIFEMKSEGLVEVANPSQIFLSNRQANTVGSAVTAVLEGSRPLLVEVQALTNTTSFGQPR
RTANGVDFNRTIMIAAVLSKRLSMRLGTQDIIVNATGGIRLDEPAADLAIALAIASSYRDIGVCPETIALGEIGLSGELR
TIPHLERRLSEASRLGFTRALVPAGANCQNINIHGIQIIAVSTVKEAIKLALTGVKTETEDVFE

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=56330 BTF_RS00270 WP_012983997.1 54028..55422(+) (radA) [Dehalococcoides mccartyi BTF08]
ATGGATAAAAGCCGTAATGTTTATATCTGTTCCAACTGCGGACATGAGAGCCTGAAGTGGCTGGGGCGTTGCCCCGGCTG
TCAGGAATGGAATACTCTTGAAGAAACAACAATTGCTGCACCTCTCGGGCGTAAAAATGCACCTGCCCGGGTTATCAGCC
CGGCAGCCGAGCTTTCCAGTCTGAATGCATCAGATACTACCCGCCGGAGTCTATCTATTTCAGAGTTCAACCGGGTTTTG
GGCGGTGGAATAGTACCGGGTTCACTGATGCTTTTAGGTGGTGAACCGGGTATCGGCAAATCCACGCTCCTTCTTCAGGT
GGCTGCCTCGGTAGCCCAGAGCGGCGGCAAAGTGGTATATGTTTCCGGGGAAGAGAACCCCGCCCAGATAAAAATGCGCG
CTCAGCGTCTGGGTATCAGCGGCGAGGGGCTTTTCCTTATGGCTGAGACAGACCTTAATGCCATTCTAGCCCAGCTTTCA
GTCCTCTGCCCGTCATTGGTTGTTATAGACTCTATCCAGACTGTATTCCTGCCTGAGCTTGAGGCCGCACCCGGTGCTAT
AAATCAGGTGCGTGAATCAGCCCTCCGCCTGATGCAGTGGGCTAAGAGCAGCGGTGCCAGCGTATTTATCACCGCCCATG
TTACCAAAGAGGGCAACATCGCCGGGCCGCGCATACTGGAACACATAGTAGATGTGGTCATGTACTTTGAGGGCGAGTCC
CAGAGTGCTTACCGTTTGATACGTTCGGTCAAAAACCGCTTCGGTTCTACCAACGAAGTAGGCATATTTGAAATGAAAAG
TGAAGGATTGGTGGAAGTAGCCAATCCCTCGCAGATATTTTTATCTAATCGGCAGGCAAACACTGTTGGTTCAGCTGTAA
CAGCAGTGCTGGAGGGTAGCCGCCCTTTACTGGTAGAGGTGCAGGCACTCACCAATACCACCAGTTTCGGCCAGCCGCGC
CGCACGGCCAACGGGGTAGATTTTAACCGTACTATTATGATAGCCGCTGTTCTTTCCAAACGCCTTTCCATGCGGCTGGG
TACCCAGGACATAATAGTAAATGCCACCGGCGGTATTCGTCTGGACGAGCCGGCCGCAGATTTGGCTATTGCTTTGGCCA
TTGCCTCCAGTTACCGTGATATAGGGGTCTGCCCGGAAACTATAGCACTGGGTGAGATTGGCCTTTCAGGGGAACTGAGG
ACAATCCCTCATTTGGAAAGACGTCTTTCCGAGGCCAGCCGTTTGGGTTTTACGAGGGCTTTGGTACCTGCCGGTGCTAA
TTGCCAGAATATAAATATACATGGTATCCAGATTATTGCGGTTTCAACTGTCAAAGAGGCTATTAAACTGGCGCTTACCG
GGGTAAAAACGGAGACCGAAGATGTTTTTGAATGA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(335-424)

AAA domain (AAA_25)

(66-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1S6SF54

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.218

98.707

0.496
  radA Streptococcus mitis SK321

48.578

98.491

0.478
  radA Streptococcus mitis NCTC 12261

48.359

98.491

0.476
  radA Streptococcus pneumoniae TIGR4

48.14

98.491

0.474
  radA Streptococcus pneumoniae R6

48.14

98.491

0.474
  radA Streptococcus pneumoniae Rx1

48.14

98.491

0.474
  radA Streptococcus pneumoniae D39

48.14

98.491

0.474

Multiple sequence alignment