Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L6L57_RS03650 Genome accession   NZ_CP091712
Coordinates   800770..801210 (-) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain KFu014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 795770..806210
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6L57_RS03625 (L6L57_03625) coaE 796260..796880 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  L6L57_RS03630 (L6L57_03630) - 796905..796949 (+) 45 WP_120795372.1 protein YacM -
  L6L57_RS03635 (L6L57_03635) guaC 797105..798148 (+) 1044 WP_001217333.1 GMP reductase -
  L6L57_RS03640 (L6L57_03640) hofC 798183..799385 (-) 1203 WP_000157219.1 protein transport protein HofC -
  L6L57_RS03645 (L6L57_03645) pilB 799375..800760 (-) 1386 WP_001025211.1 type II secretion system protein GspE Machinery gene
  L6L57_RS03650 (L6L57_03650) pilA 800770..801210 (-) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  L6L57_RS03655 (L6L57_03655) nadC 801413..802306 (-) 894 WP_001135167.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L6L57_RS03660 (L6L57_03660) ampD 802394..802945 (+) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L6L57_RS03665 (L6L57_03665) ampE 802942..803796 (+) 855 WP_000172020.1 beta-lactamase regulator AmpE -
  L6L57_RS03670 (L6L57_03670) aroP 803839..805212 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=563259 L6L57_RS03650 WP_000360914.1 800770..801210(-) (pilA) [Escherichia coli strain KFu014]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=563259 L6L57_RS03650 WP_000360914.1 800770..801210(-) (pilA) [Escherichia coli strain KFu014]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGTATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363