Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   H175_RS26320 Genome accession   NC_020376
Coordinates   5160800..5162149 (-) Length   449 a.a.
NCBI ID   WP_000879411.1    Uniprot ID   A0AAN4HIN4
Organism   Bacillus thuringiensis serovar thuringiensis str. IS5056     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5155800..5167149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H175_RS26300 (H175_ch5301) secA 5156125..5158632 (-) 2508 WP_000579372.1 preprotein translocase subunit SecA -
  H175_RS26305 (H175_ch5302) raiA 5158906..5159448 (-) 543 WP_000671189.1 ribosome-associated translation inhibitor RaiA -
  H175_RS26310 (H175_ch5304) cspC 5159772..5159969 (-) 198 WP_001990088.1 cold shock protein CspC -
  H175_RS26315 (H175_ch5305) - 5160096..5160800 (-) 705 WP_021036092.1 ComF family protein -
  H175_RS26320 (H175_ch5306) comFA 5160800..5162149 (-) 1350 WP_000879411.1 ATP-dependent helicase ComFA Machinery gene
  H175_RS26325 (H175_ch5307) - 5162277..5163704 (-) 1428 WP_000499486.1 C40 family peptidase -
  H175_RS26330 (H175_ch5308) - 5163853..5164167 (-) 315 WP_000400857.1 helix-turn-helix domain-containing protein -
  H175_RS26335 (H175_ch5309) - 5164340..5165182 (-) 843 WP_000684727.1 DegV family protein -
  H175_RS26340 (H175_ch5311) - 5165419..5166054 (+) 636 WP_000926670.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51043.86 Da        Isoelectric Point: 9.9151

>NTDB_id=56313 H175_RS26320 WP_000879411.1 5160800..5162149(-) (comFA) [Bacillus thuringiensis serovar thuringiensis str. IS5056]
MLAGKQLLLDELSSDLQRELNDLKKKGEVVCVQGVKKKASKYVCQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIAERKREKNLNLLRWSGTLSTGQNLAAQGVIEAIKRKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KERAARIYLTATPDETWKQKFRRGEQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLIHKRIPRVLLQWLQTYLNKKYPIFL
FVPHVRYIEEISLLLKSLNKRIEGVHAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVVYFHYGKTEAMVHAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=56313 H175_RS26320 WP_000879411.1 5160800..5162149(-) (comFA) [Bacillus thuringiensis serovar thuringiensis str. IS5056]
ATGCTAGCGGGGAAACAGTTGCTATTAGACGAACTTTCTTCAGATTTACAGAGGGAATTAAATGATTTGAAAAAGAAGGG
AGAGGTCGTGTGTGTACAAGGTGTAAAAAAGAAGGCTTCTAAATATGTATGTCAGCGCTGTGGAAATATAGAACAGCGGC
TATTTGCGTCGTTTTTATGTAAAAGATGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAACGATGGGGAGGGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTTAAACTTGTTACGGTGGAGCGGAACGTT
GTCTACTGGTCAGAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAAGCGAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCAACCACGCATCAATTATTGCGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAGAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCAAAAATTTAGAAGAGGTGAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
GCCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACGTACTTAAATAAAAAATATCCTATTTTTTTA
TTTGTCCCCCATGTGCGATATATAGAAGAGATAAGCCTGTTGTTAAAGTCATTAAACAAGCGAATTGAAGGTGTACATGC
AGAAGATCCGGGTAGAAAAGAAAAAGTAGCGGCTTTCAGAAAAGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACAGTAAAAAATTTGCAAGTAGCGGTTTTAGGGGCGGAAGAAGAAATATTCTCAGAAAGTGCACTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCGTTTATTTTCACTATGGTAAGACAGAGGC
GATGGTGCACGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(108-254)

Helicase conserved C-terminal domain (Helicase_C)

(312-408)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.452

98.441

0.497
  comFA Latilactobacillus sakei subsp. sakei 23K

41.457

88.641

0.367

Multiple sequence alignment