Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   L6L60_RS00540 Genome accession   NZ_CP091703
Coordinates   113154..114578 (-) Length   474 a.a.
NCBI ID   WP_000148130.1    Uniprot ID   -
Organism   Escherichia coli strain KOr021     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 108154..119578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6L60_RS00510 (L6L60_00510) yeeZ 108209..109033 (+) 825 WP_000754737.1 SDR family oxidoreductase -
  L6L60_RS00515 (L6L60_00515) yeeY 109079..110008 (+) 930 WP_000803363.1 LysR substrate-binding domain-containing protein -
  L6L60_RS00520 (L6L60_00520) yoeI 110223..110285 (+) 63 WP_010723108.1 membrane protein YoeI -
  L6L60_RS00525 (L6L60_00525) plaP 110275..111633 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  L6L60_RS00530 (L6L60_00530) tsuA 111812..112870 (+) 1059 WP_000492348.1 thiosulfate utilization transporter TsuA/YeeE -
  L6L60_RS00535 (L6L60_00535) tsuB 112884..113111 (+) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  L6L60_RS00540 (L6L60_00540) sbcB 113154..114578 (-) 1425 WP_000148130.1 exodeoxyribonuclease I Machinery gene
  L6L60_RS00545 (L6L60_00545) - 114760..115938 (+) 1179 WP_001007952.1 site-specific integrase -
  L6L60_RS00550 (L6L60_00550) - 115919..116110 (-) 192 WP_000132739.1 AlpA family phage regulatory protein -
  L6L60_RS00555 (L6L60_00555) yfdT 116199..116504 (-) 306 WP_000627693.1 protein YfdT -
  L6L60_RS00560 (L6L60_00560) - 116504..116866 (-) 363 WP_001242744.1 hypothetical protein -
  L6L60_RS00565 (L6L60_00565) yfdR 116857..117393 (-) 537 WP_000008235.1 5'-deoxynucleotidase -
  L6L60_RS00570 (L6L60_00570) - 117475..117794 (-) 320 Protein_113 hypothetical protein -
  L6L60_RS00575 (L6L60_00575) - 117907..118194 (+) 288 WP_000141754.1 hypothetical protein -
  L6L60_RS00580 (L6L60_00580) - 118107..118415 (+) 309 Protein_115 phage tail protein -
  L6L60_RS00585 (L6L60_00585) - 118415..119008 (+) 594 WP_000805577.1 tail fiber assembly protein -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54323.60 Da        Isoelectric Point: 5.6054

>NTDB_id=563083 L6L60_RS00540 WP_000148130.1 113154..114578(-) (sbcB) [Escherichia coli strain KOr021]
MTGTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENE
AAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRG
NTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDAD
RLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDK
RIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAQEIV

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=563083 L6L60_RS00540 WP_000148130.1 113154..114578(-) (sbcB) [Escherichia coli strain KOr021]
ATGACAGGCACAGATAAACAACCTACCTTCCTCTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCGCCC
TGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCGG
ATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAA
GCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA
CGATGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCT
GGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTA
CCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTA
CGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATAAAC
ACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGTGGC
AATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGACAT
TTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCG
CCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGAC
CGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGTGGT
GGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTGACG
CCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAA
CGAATTGAAAAACTGTTGTTCAATTATCGAGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCT
TGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTACGCTGATGAATTGCAGATGCTGGCACAACAGTATG
CCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGCAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease (RNase_T)

(12-187)

Exonuclease C-terminal (ExoI_SH3)

(211-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

99.156

0.584