Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   H175_RS21715 Genome accession   NC_020376
Coordinates   4292498..4293244 (-) Length   248 a.a.
NCBI ID   WP_000487009.1    Uniprot ID   -
Organism   Bacillus thuringiensis serovar thuringiensis str. IS5056     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 4287498..4298244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H175_RS21700 (H175_ch4379) dnaG 4288259..4290055 (-) 1797 WP_000528221.1 DNA primase -
  H175_RS37325 (H175_ch4380) - 4290137..4290280 (-) 144 WP_000479733.1 hypothetical protein -
  H175_RS21705 (H175_ch4382) - 4290916..4291728 (-) 813 WP_000368944.1 pyruvate, water dikinase regulatory protein -
  H175_RS21710 (H175_ch4383) - 4291758..4292390 (-) 633 WP_000583756.1 helix-turn-helix transcriptional regulator -
  H175_RS21715 (H175_ch4384) recO 4292498..4293244 (-) 747 WP_000487009.1 DNA repair protein RecO Machinery gene
  H175_RS21720 (H175_ch4385) - 4293278..4293421 (-) 144 WP_000883924.1 YqzL family protein -
  H175_RS21725 (H175_ch4386) era 4293598..4294503 (-) 906 WP_001080230.1 GTPase Era -
  H175_RS21730 (H175_ch4387) - 4294496..4294894 (-) 399 WP_001086285.1 cytidine deaminase -
  H175_RS21735 - 4294994..4295347 (-) 354 WP_000715463.1 diacylglycerol kinase family protein -
  H175_RS21740 (H175_ch4389) ybeY 4295344..4295814 (-) 471 WP_000054685.1 rRNA maturation RNase YbeY -
  H175_RS21745 (H175_ch4390) - 4295811..4297955 (-) 2145 WP_000091512.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28618.21 Da        Isoelectric Point: 8.0566

>NTDB_id=56303 H175_RS21715 WP_000487009.1 4292498..4293244(-) (recO) [Bacillus thuringiensis serovar thuringiensis str. IS5056]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDPYRIPVGEAVHKLLRLFFHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=56303 H175_RS21715 WP_000487009.1 4292498..4293244(-) (recO) [Bacillus thuringiensis serovar thuringiensis str. IS5056]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAACTTGGTAAGGTAAGTGCAATGGCAAGAGGGGCAAAAAAACCGAAAAGCCGATTAGCATCTGTGTCGCAATTAATGA
CACATGGCCATTTTCTTATTCAAATGGGATCTGGGCTTGGAACATTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCGTATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTATCAAACGAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAC
TTTGTCGCCTTCTCCGTAAGGGAAGGCGGTTTTCTGTGCTCACGTCATGCAGAGCAAGACCCATATCGTATACCGGTAGG
AGAAGCTGTTCATAAATTATTACGTCTTTTCTTCCATTTCGATTTACATAGGCTCGGAAATGTATCGGTAAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGAATTTATTTGAAATCAAGACGTTTTTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-77)

Recombination protein O C terminal (RecO_C)

(82-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment