Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   H175_RS20220 Genome accession   NC_020376
Coordinates   4027659..4028093 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis serovar thuringiensis str. IS5056     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4022659..4033093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H175_RS20205 (H175_ch4072) - 4023837..4024394 (+) 558 WP_000062063.1 PadR family transcriptional regulator -
  H175_RS20210 (H175_ch4073) metE 4024906..4027194 (+) 2289 WP_001007616.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  H175_RS20215 (H175_ch4074) - 4027233..4027622 (-) 390 WP_000424024.1 competence protein ComJ -
  H175_RS20220 (H175_ch4075) nucA/comI 4027659..4028093 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  H175_RS20225 (H175_ch4076) - 4028320..4029069 (+) 750 WP_000388467.1 DUF3967 domain-containing protein -
  H175_RS20230 (H175_ch4077) kinB 4029108..4030382 (-) 1275 WP_000420372.1 sporulation sensor histidine kinase KinB -
  H175_RS20235 (H175_ch4079) - 4030693..4030893 (+) 201 WP_000929276.1 hypothetical protein -
  H175_RS20240 (H175_ch4080) - 4031016..4032179 (+) 1164 WP_000434575.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=56299 H175_RS20220 WP_000811494.1 4027659..4028093(-) (nucA/comI) [Bacillus thuringiensis serovar thuringiensis str. IS5056]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=56299 H175_RS20220 WP_000811494.1 4027659..4028093(-) (nucA/comI) [Bacillus thuringiensis serovar thuringiensis str. IS5056]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGGGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment