Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L6L65_RS13395 Genome accession   NZ_CP091691
Coordinates   2750506..2750946 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain KMi006     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2745506..2755946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6L65_RS13375 (L6L65_13370) aroP 2746504..2747877 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  L6L65_RS13380 (L6L65_13375) ampE 2747920..2748774 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  L6L65_RS13385 (L6L65_13380) ampD 2748771..2749322 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L6L65_RS13390 (L6L65_13385) nadC 2749410..2750303 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L6L65_RS13395 (L6L65_13390) pilA 2750506..2750946 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  L6L65_RS13400 (L6L65_13395) pilB 2750956..2752341 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  L6L65_RS13405 (L6L65_13400) hofC 2752331..2753533 (+) 1203 WP_000157284.1 protein transport protein HofC -
  L6L65_RS13410 (L6L65_13405) guaC 2753568..2754611 (-) 1044 WP_001217338.1 GMP reductase -
  L6L65_RS13415 (L6L65_13410) - 2754767..2754811 (-) 45 WP_120795372.1 protein YacM -
  L6L65_RS13420 (L6L65_13415) coaE 2754836..2755456 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=562951 L6L65_RS13395 WP_000360904.1 2750506..2750946(+) (pilA) [Escherichia coli strain KMi006]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=562951 L6L65_RS13395 WP_000360904.1 2750506..2750946(+) (pilA) [Escherichia coli strain KMi006]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363