Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L6L66_RS20360 Genome accession   NZ_CP091677
Coordinates   4162300..4163796 (-) Length   498 a.a.
NCBI ID   WP_001040683.1    Uniprot ID   -
Organism   Escherichia coli strain KFu022     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4157300..4168796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L6L66_RS20335 (L6L66_20335) panM 4157523..4157906 (+) 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
  L6L66_RS20340 (L6L66_20340) livJ 4158105..4159208 (-) 1104 WP_001022007.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  L6L66_RS20345 (L6L66_20345) rpoH 4159479..4160333 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  L6L66_RS20350 (L6L66_20350) ftsX 4160578..4161636 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  L6L66_RS20355 (L6L66_20355) ftsE 4161629..4162297 (-) 669 WP_000617726.1 cell division ATP-binding protein FtsE -
  L6L66_RS20360 (L6L66_20360) pilA 4162300..4163796 (-) 1497 WP_001040683.1 signal recognition particle-docking protein FtsY Machinery gene
  L6L66_RS20365 (L6L66_20365) rsmD 4163946..4164542 (+) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  L6L66_RS20370 (L6L66_20370) yhhL 4164532..4164801 (+) 270 WP_001295207.1 DUF1145 family protein -
  L6L66_RS20375 (L6L66_20375) yhhM 4164804..4165163 (-) 360 WP_000042903.1 DUF2500 domain-containing protein -
  L6L66_RS20380 (L6L66_20380) yhhN 4165304..4165930 (+) 627 WP_000964718.1 lysoplasmalogenase -
  L6L66_RS20385 (L6L66_20385) zntA 4166004..4168202 (+) 2199 WP_000106538.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  L6L66_RS20390 (L6L66_20390) tusA 4168304..4168549 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54617.41 Da        Isoelectric Point: 4.1560

>NTDB_id=562817 L6L66_RS20360 WP_001040683.1 4162300..4163796(-) (pilA) [Escherichia coli strain KFu022]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQSVVEEIVQVQEPAKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAGVVAQPEPVVEETPEPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=562817 L6L66_RS20360 WP_001040683.1 4162300..4163796(-) (pilA) [Escherichia coli strain KFu022]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAATCGGTTGTAGAAGAAATCGTTCAGGTGCAAGAGCCTGCGAAGGCTTCTGAACAAGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTCACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGGAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAGCTGCCGTTGCCGGAAGACGTCAACGCCGAAGAGGTTTCGCCAGAAGAGTGGCAGGCTGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCTGGAAGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTTTTGATCGCCGAT
GTGGGCGTGGAAACCACACGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCACGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCGGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCCTCTGTTATCTTCGACGCCATTCAGGCAGCTAAAG
CGCGTAATATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAGAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCGCCGCATGAAGTTATGCTGACTATCGATGCCAGCACCGGGCAGAA
CGCGGTAAGCCAGGCCAAACTGTTCCATGAAGCCGTTGGTTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)

SRP54-type protein, GTPase domain (SRP54)

(294-494)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378