Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L3Z43_RS03260 Genome accession   NZ_CP091427
Coordinates   677411..677851 (-) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain 1000C-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 672411..682851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3Z43_RS03235 coaE 672901..673521 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  L3Z43_RS03240 - 673546..673590 (+) 45 WP_120795372.1 protein YacM -
  L3Z43_RS03245 guaC 673746..674789 (+) 1044 WP_001217338.1 GMP reductase -
  L3Z43_RS03250 hofC 674824..676026 (-) 1203 WP_000157266.1 protein transport protein HofC -
  L3Z43_RS03255 pilB 676016..677401 (-) 1386 WP_001025146.1 type II secretion system protein GspE Machinery gene
  L3Z43_RS03260 pilA 677411..677851 (-) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  L3Z43_RS03265 nadC 678054..678947 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L3Z43_RS03270 ampD 679035..679586 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L3Z43_RS03275 ampE 679583..680437 (+) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  L3Z43_RS03280 aroP 680480..681853 (-) 1374 WP_000969915.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=561292 L3Z43_RS03260 WP_000360904.1 677411..677851(-) (pilA) [Escherichia coli strain 1000C-3]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=561292 L3Z43_RS03260 WP_000360904.1 677411..677851(-) (pilA) [Escherichia coli strain 1000C-3]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363