Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KFV12_RS12645 Genome accession   NZ_CP073819
Coordinates   2371827..2373188 (-) Length   453 a.a.
NCBI ID   WP_096077851.1    Uniprot ID   A0A2G5NU81
Organism   Macrococcus epidermidis strain Epi3002-OL     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2366827..2378188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFV12_RS12620 (KFV12_12660) cysE 2367251..2367898 (-) 648 WP_199798240.1 serine O-acetyltransferase -
  KFV12_RS12625 (KFV12_12665) gltX 2368142..2369596 (-) 1455 WP_254257661.1 glutamate--tRNA ligase -
  KFV12_RS12630 (KFV12_12670) ispF 2369607..2370089 (-) 483 WP_254256914.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  KFV12_RS12635 (KFV12_12675) ispD 2370086..2370757 (-) 672 WP_308192413.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  KFV12_RS12640 (KFV12_12680) - 2370759..2371814 (-) 1056 WP_111717459.1 PIN/TRAM domain-containing protein -
  KFV12_RS12645 (KFV12_12685) radA 2371827..2373188 (-) 1362 WP_096077851.1 DNA repair protein RadA Machinery gene
  KFV12_RS12650 (KFV12_12690) - 2373341..2375764 (-) 2424 WP_099577328.1 ATP-dependent Clp protease ATP-binding subunit -
  KFV12_RS12655 (KFV12_12695) - 2375777..2376787 (-) 1011 WP_111717457.1 protein arginine kinase -
  KFV12_RS12660 (KFV12_12700) - 2376777..2377169 (-) 393 WP_254256915.1 UvrB/UvrC motif-containing protein -
  KFV12_RS12665 (KFV12_12705) - 2377181..2377642 (-) 462 WP_099577322.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 50248.92 Da        Isoelectric Point: 7.4857

>NTDB_id=560976 KFV12_RS12645 WP_096077851.1 2371827..2373188(-) (radA) [Macrococcus epidermidis strain Epi3002-OL]
MAKVKSTFECMACGYQSPKWMGKCPNCGAWNQMEEVIEHKQKGPKTAIHQQDSNSKVEKLKNITKESVPRDHTQMGELDR
VLGGGIVPGSLVLIGGDPGIGKSTLLLQVCAMLSQRHPVLYISGEESVRQTKLRADRLKEDAGELDVYAETNLQIIHETV
KKMQPKFLVIDSIQTIFHPEVTSAPGSVSQVRECTQELMRIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
THHSYRILRAVKNRFGSTNEMGIFEMRNTGLTEVLNPSEMFLEERTKNVAGSTIVATMEGTRPLLVEVQSLVSPTSFHNP
RRMATGIDHNRLTLLMAVLEKKQGYLLQQQDAYVKVAGGVKLDEPAVDLSIIISIASSYNDKPTRGDDCFIGEVGLTGEV
RRVARIEQRVQEAEKLGFKRVIIPKNNIGGWDFPGNIEVIGVTNISEALKLAF

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=560976 KFV12_RS12645 WP_096077851.1 2371827..2373188(-) (radA) [Macrococcus epidermidis strain Epi3002-OL]
TTGGCTAAAGTGAAATCAACTTTTGAATGTATGGCCTGTGGTTATCAGTCCCCAAAATGGATGGGTAAATGTCCGAATTG
TGGCGCCTGGAATCAGATGGAAGAAGTCATCGAACATAAACAAAAAGGACCAAAAACAGCAATACATCAGCAAGATTCAA
ATTCTAAAGTAGAAAAATTAAAAAATATAACGAAAGAAAGTGTGCCAAGAGACCATACGCAAATGGGAGAACTTGACCGT
GTATTAGGTGGCGGTATTGTCCCTGGCTCACTCGTGCTCATTGGTGGCGATCCTGGTATCGGAAAGTCAACATTGTTACT
GCAAGTGTGTGCGATGTTGTCACAACGTCATCCTGTACTTTATATATCTGGTGAGGAATCGGTAAGACAAACAAAACTCC
GTGCAGATCGCTTAAAGGAAGATGCTGGAGAATTAGATGTGTATGCGGAGACAAATCTTCAAATCATCCATGAAACCGTG
AAGAAGATGCAGCCGAAGTTTTTAGTGATTGACTCGATTCAAACGATTTTTCATCCGGAAGTAACGAGCGCACCAGGTTC
AGTATCTCAAGTGCGTGAATGTACGCAGGAGTTAATGCGTATTGCAAAGCAGATGAATATTGCGACCTTTATCGTCGGTC
ACGTTACAAAAGAAGGGCAAATCGCAGGACCACGTTTACTTGAACATATGGTGGATACTGTACTTTATTTTGAGGGCGAT
ACACATCACAGTTACCGTATTTTACGCGCCGTGAAAAACCGTTTCGGTTCTACAAATGAAATGGGCATTTTTGAAATGCG
CAATACTGGCCTTACAGAAGTATTAAACCCGAGCGAAATGTTCTTAGAAGAACGTACGAAGAATGTAGCTGGGTCTACGA
TTGTTGCGACAATGGAAGGCACACGTCCACTCCTTGTTGAAGTTCAATCATTGGTATCACCTACATCATTTCATAACCCT
AGACGTATGGCGACGGGTATTGATCACAATCGTTTAACACTGTTAATGGCAGTATTAGAGAAGAAACAAGGTTATCTGTT
ACAGCAACAGGATGCCTATGTCAAAGTAGCAGGTGGTGTGAAATTAGATGAACCCGCTGTTGATTTAAGTATTATTATTA
GCATTGCGTCAAGTTATAATGATAAACCAACCCGTGGCGATGACTGCTTTATCGGAGAAGTAGGTTTGACTGGAGAAGTA
AGACGTGTTGCGCGTATTGAACAACGCGTGCAGGAAGCAGAAAAACTTGGATTCAAACGTGTCATTATTCCAAAGAACAA
TATCGGTGGCTGGGATTTCCCAGGGAATATTGAAGTGATTGGTGTAACAAACATAAGCGAAGCATTAAAGCTTGCATTTT
AA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(343-427)

AAA domain (AAA_25)

(69-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2G5NU81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

68.202

100

0.687
  radA Streptococcus pneumoniae Rx1

62.031

100

0.62
  radA Streptococcus pneumoniae D39

62.031

100

0.62
  radA Streptococcus pneumoniae R6

62.031

100

0.62
  radA Streptococcus pneumoniae TIGR4

62.031

100

0.62
  radA Streptococcus mitis NCTC 12261

62.031

100

0.62
  radA Streptococcus mitis SK321

62.031

100

0.62