Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KFV11_RS10860 Genome accession   NZ_CP073809
Coordinates   2032981..2034345 (-) Length   454 a.a.
NCBI ID   WP_149459308.1    Uniprot ID   -
Organism   Macrococcus equipercicus strain Epi0143-OL     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2027981..2039345
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFV11_RS10835 (KFV11_10830) cysE 2028391..2029038 (-) 648 WP_211359998.1 serine O-acetyltransferase -
  KFV11_RS10840 (KFV11_10835) gltX 2029293..2030747 (-) 1455 WP_254249931.1 glutamate--tRNA ligase -
  KFV11_RS10845 (KFV11_10840) ispF 2030763..2031242 (-) 480 WP_149459305.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  KFV11_RS10850 (KFV11_10845) ispD 2031242..2031919 (-) 678 WP_254250624.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  KFV11_RS10855 (KFV11_10850) - 2031916..2032968 (-) 1053 WP_254249932.1 PIN/TRAM domain-containing protein -
  KFV11_RS10860 (KFV11_10855) radA 2032981..2034345 (-) 1365 WP_149459308.1 DNA repair protein RadA Machinery gene
  KFV11_RS10865 (KFV11_10860) - 2034481..2036922 (-) 2442 WP_149459309.1 ATP-dependent Clp protease ATP-binding subunit -
  KFV11_RS10870 (KFV11_10865) - 2036935..2037942 (-) 1008 WP_149459310.1 protein arginine kinase -
  KFV11_RS10875 (KFV11_10870) - 2037943..2038323 (-) 381 WP_149459311.1 UvrB/UvrC motif-containing protein -
  KFV11_RS10880 (KFV11_10875) - 2038340..2038798 (-) 459 WP_149459312.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 50033.58 Da        Isoelectric Point: 7.6797

>NTDB_id=560944 KFV11_RS10860 WP_149459308.1 2032981..2034345(-) (radA) [Macrococcus equipercicus strain Epi0143-OL]
MAKTKSTFECMACGYQSPKWMGKCPNCGAWNQMEEVIEHKAKGPKTAITESSSSERVEKLKNITKESTPRSTTYIREFDR
VLGGGIVPGSLILIGGDPGIGKSTLLLQVCALLSKDNAVLYISGEESVRQTKLRADRLKEDAGELSVYAETNLQIIHETV
KKTNPKFLVIDSIQTIFHPEVTSAPGSVSQVRECTQELMRMAKNMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
THHSYRILRAVKNRFGSTNEMGIFEMRNTGLSEVLNPSEMFLEERTKNVAGSTIVATMEGTRPLLVEVQALVTPTTFHNP
RRMATGIDHNRLSLLMAVLEKKQGLLLQQQDAYIKVAGGVKLDEPAVDLSVIISIASSFNDKPTRGDDCFIGEVGLTGEV
RRVARIEQRVQEAAKLGFKRAVIPKNNIGGWDFPDGIEIVGITNISEALRLAFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=560944 KFV11_RS10860 WP_149459308.1 2032981..2034345(-) (radA) [Macrococcus equipercicus strain Epi0143-OL]
TTGGCGAAAACGAAATCAACGTTTGAATGCATGGCCTGCGGGTATCAATCTCCTAAATGGATGGGCAAATGCCCGAACTG
CGGTGCCTGGAACCAGATGGAAGAAGTCATAGAACATAAAGCCAAAGGACCAAAAACCGCAATCACTGAATCATCTTCCA
GTGAACGCGTCGAAAAACTTAAAAACATCACTAAAGAGAGCACGCCGCGCTCTACAACATATATTCGTGAATTTGACCGC
GTGCTCGGCGGCGGCATTGTGCCGGGCTCGCTTATTTTAATCGGCGGGGACCCGGGTATCGGGAAATCAACGTTACTGCT
GCAAGTATGTGCCCTCCTATCAAAGGATAACGCCGTGCTTTATATTTCCGGTGAGGAATCGGTCAGACAGACAAAACTGC
GCGCTGACCGGCTGAAAGAAGACGCCGGTGAACTGAGCGTCTATGCAGAAACGAACCTCCAGATCATCCACGAAACAGTG
AAGAAGACAAATCCGAAATTTCTTGTCATCGACTCCATTCAGACGATTTTCCACCCCGAAGTGACGTCAGCACCCGGTTC
TGTATCACAGGTGAGAGAATGTACGCAGGAATTAATGCGCATGGCGAAGAACATGAATATCGCAACGTTCATCGTCGGTC
ACGTGACGAAAGAAGGCCAGATTGCCGGGCCGCGGCTGCTGGAGCATATGGTGGATACTGTACTCTATTTTGAAGGCGAT
ACACATCACAGCTACCGTATACTGCGGGCAGTGAAGAACCGTTTCGGCTCCACGAATGAAATGGGTATTTTTGAAATGCG
GAATACGGGGCTGAGCGAAGTACTGAATCCGTCAGAGATGTTTCTTGAAGAACGGACAAAAAACGTCGCAGGTTCCACGA
TTGTCGCGACGATGGAAGGAACGCGGCCGCTGCTTGTTGAAGTGCAGGCGCTGGTCACACCGACGACATTCCACAATCCG
CGGCGTATGGCCACAGGGATTGACCATAACCGGTTGAGCTTACTGATGGCAGTGCTTGAGAAGAAGCAGGGCTTACTGCT
GCAGCAGCAGGATGCTTATATTAAAGTTGCAGGCGGGGTCAAACTGGATGAACCGGCAGTTGATTTGAGCGTCATTATTA
GTATCGCTTCAAGTTTCAATGATAAACCGACGCGTGGTGATGACTGTTTCATTGGTGAGGTCGGCCTGACAGGAGAAGTA
AGGCGTGTCGCCCGCATCGAGCAGCGCGTACAGGAAGCGGCGAAACTTGGTTTCAAGCGTGCTGTCATACCGAAGAATAA
CATTGGCGGCTGGGACTTCCCGGACGGCATTGAGATTGTTGGTATTACAAATATTTCTGAAGCGTTGAGACTGGCATTTA
ATTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(356-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(75-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

68.49

100

0.689
  radA Streptococcus pneumoniae Rx1

61.454

100

0.615
  radA Streptococcus pneumoniae D39

61.454

100

0.615
  radA Streptococcus pneumoniae R6

61.454

100

0.615
  radA Streptococcus pneumoniae TIGR4

61.454

100

0.615
  radA Streptococcus mitis NCTC 12261

61.454

100

0.615
  radA Streptococcus mitis SK321

61.454

100

0.615