Detailed information    

insolico Bioinformatically predicted

Overview

Name   ccrB   Type   Machinery gene
Locus tag   KFV10_RS00225 Genome accession   NZ_CP073808
Coordinates   43349..44974 (-) Length   541 a.a.
NCBI ID   WP_254253893.1    Uniprot ID   -
Organism   Macrococcoides canis strain IMD0709     
Function   promote SCCmec transfer (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 30759..54793 43349..44974 within 0
Prophage 1619..54793 43349..44974 within 0


Gene organization within MGE regions


Location: 1619..54793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFV10_RS00010 (KFV10_00010) dnaN 1619..2752 (+) 1134 WP_236366083.1 DNA polymerase III subunit beta -
  KFV10_RS00015 (KFV10_00015) - 2852..3748 (+) 897 WP_236366084.1 ABC transporter ATP-binding protein -
  KFV10_RS00020 (KFV10_00020) - 3741..4982 (+) 1242 WP_254253882.1 ABC transporter permease -
  KFV10_RS00025 (KFV10_00025) - 5072..5776 (+) 705 WP_086041412.1 DsbA family protein -
  KFV10_RS00030 (KFV10_00030) - 5776..6192 (+) 417 WP_086041413.1 disulfide oxidoreductase -
  KFV10_RS00035 (KFV10_00035) yaaA 6331..6552 (+) 222 WP_086041414.1 S4 domain-containing protein YaaA -
  KFV10_RS00040 (KFV10_00040) recF 6549..7658 (+) 1110 WP_236366085.1 DNA replication/repair protein RecF -
  KFV10_RS00045 (KFV10_00045) gyrB 7666..9594 (+) 1929 WP_236366086.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  KFV10_RS00050 (KFV10_00050) gyrA 9622..12276 (+) 2655 WP_086041416.1 DNA gyrase subunit A -
  KFV10_RS00055 (KFV10_00055) alsS 12472..14139 (+) 1668 WP_086041417.1 acetolactate synthase AlsS -
  KFV10_RS00060 (KFV10_00060) budA 14150..14851 (+) 702 WP_086041418.1 acetolactate decarboxylase -
  KFV10_RS00065 (KFV10_00065) - 15003..15821 (-) 819 WP_086041419.1 NAD(P)H-hydrate dehydratase -
  KFV10_RS00070 (KFV10_00070) serS 16166..17437 (+) 1272 WP_236366089.1 serine--tRNA ligase -
  KFV10_RS00075 (KFV10_00075) - 17666..18181 (+) 516 WP_086041421.1 YceI family protein -
  KFV10_RS00080 (KFV10_00080) - 18315..19226 (+) 912 WP_086041422.1 DUF2232 domain-containing protein -
  KFV10_RS00085 (KFV10_00085) - 19256..21223 (+) 1968 WP_086041423.1 DHH family phosphoesterase -
  KFV10_RS00090 (KFV10_00090) rplI 21226..21672 (+) 447 WP_086041424.1 50S ribosomal protein L9 -
  KFV10_RS00095 (KFV10_00095) dnaB 21690..23096 (+) 1407 WP_086041425.1 replicative DNA helicase -
  KFV10_RS00100 (KFV10_00100) - 23419..24708 (+) 1290 WP_210152336.1 adenylosuccinate synthase -
  KFV10_RS00105 (KFV10_00105) - 24792..25679 (-) 888 WP_210152337.1 YitT family protein -
  KFV10_RS00120 (KFV10_00120) yycF 26100..26801 (+) 702 WP_164941339.1 response regulator YycF -
  KFV10_RS00125 (KFV10_00125) walK 26806..28644 (+) 1839 WP_254253883.1 cell wall metabolism sensor histidine kinase WalK -
  KFV10_RS00130 (KFV10_00130) - 28637..29950 (+) 1314 WP_211552419.1 YycH family regulatory protein -
  KFV10_RS00135 (KFV10_00135) - 29950..30741 (+) 792 WP_086041431.1 two-component system regulatory protein YycI -
  KFV10_RS00140 (KFV10_00140) - 30759..31550 (+) 792 WP_086041432.1 MBL fold metallo-hydrolase -
  KFV10_RS00145 (KFV10_00145) rlmH 31810..32289 (+) 480 WP_133421072.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  KFV10_RS00150 (KFV10_00150) - 32550..35858 (+) 3309 WP_254253884.1 Eco57I restriction-modification methylase domain-containing protein -
  KFV10_RS00155 (KFV10_00155) - 36131..36652 (-) 522 WP_254253885.1 hypothetical protein -
  KFV10_RS00160 (KFV10_00160) - 36678..37583 (-) 906 WP_254253886.1 Kiwa anti-phage protein KwaB-like domain-containing protein -
  KFV10_RS00165 (KFV10_00165) - 37743..37919 (-) 177 WP_254253887.1 hypothetical protein -
  KFV10_RS00170 (KFV10_00170) - 37922..38263 (-) 342 WP_254253888.1 hypothetical protein -
  KFV10_RS00175 (KFV10_00175) - 38278..38721 (-) 444 WP_254253889.1 hypothetical protein -
  KFV10_RS00180 (KFV10_00180) - 38744..39028 (-) 285 WP_101171199.1 hypothetical protein -
  KFV10_RS00185 (KFV10_00185) - 39043..39267 (-) 225 WP_254253890.1 hypothetical protein -
  KFV10_RS00190 (KFV10_00190) - 39287..39763 (-) 477 WP_133454735.1 hypothetical protein -
  KFV10_RS00195 (KFV10_00195) - 39732..40646 (-) 915 WP_254253891.1 helix-turn-helix transcriptional regulator -
  KFV10_RS00200 (KFV10_00200) - 40827..41270 (-) 444 WP_188017755.1 JAB domain-containing protein -
  KFV10_RS00205 (KFV10_00205) - 41293..41796 (-) 504 WP_188017756.1 DUF1643 domain-containing protein -
  KFV10_RS00210 (KFV10_00210) - 41815..42174 (-) 360 WP_254253892.1 DUF960 family protein -
  KFV10_RS00215 (KFV10_00215) - 42204..42563 (-) 360 WP_188017758.1 SAUGI family uracil-DNA glycosylase inhibitor -
  KFV10_RS00220 (KFV10_00220) - 43038..43232 (+) 195 WP_188017759.1 hypothetical protein -
  KFV10_RS00225 (KFV10_00225) ccrB 43349..44974 (-) 1626 WP_254253893.1 recombinase family protein Machinery gene
  KFV10_RS00230 (KFV10_00230) - 44977..46344 (-) 1368 WP_254253894.1 recombinase family protein -
  KFV10_RS00235 (KFV10_00235) - 46557..48113 (-) 1557 WP_254253895.1 phage/plasmid primase, P4 family -
  KFV10_RS00240 (KFV10_00240) - 48106..48669 (-) 564 WP_254253896.1 hypothetical protein -
  KFV10_RS00245 (KFV10_00245) - 48656..49903 (-) 1248 WP_254253897.1 DNA polymerase -
  KFV10_RS00250 (KFV10_00250) - 49984..50232 (-) 249 WP_254253898.1 hypothetical protein -
  KFV10_RS00255 (KFV10_00255) - 50389..52044 (+) 1656 WP_254253899.1 hypothetical protein -
  KFV10_RS00260 (KFV10_00260) - 52180..52743 (+) 564 WP_254253900.1 hypothetical protein -
  KFV10_RS00270 (KFV10_00270) - 54203..54385 (+) 183 Protein_51 IS256 family transposase -
  KFV10_RS00275 (KFV10_00275) - 54416..54793 (-) 378 WP_041636585.1 BlaI/MecI/CopY family transcriptional regulator -

Sequence

Protein


Download         Length: 541 a.a.        Molecular weight: 61633.79 Da        Isoelectric Point: 9.5201

>NTDB_id=560890 KFV10_RS00225 WP_254253893.1 43349..44974(-) (ccrB) [Macrococcoides canis strain IMD0709]
MNKKAAIYARVSTVAQAEEGFSIDGQIALLEDVCKQNGYDVYDIYVDRGISGKNMDRPALLQMNEDARQGKFQVIAVFKT
NRLARNTSDLLKMVEEYQKNNVEFLSYSEKFDTTSAAGKLMLQIISSMAEFERNQIVENVTMGLEKRAKNGYYNGNKLLG
YDKIPNDKRHLAINESEAEIVRRIFNEYANGKGYRAIANGLNKEGLRTKKGNPFDIAGVKYILNNVFYIGQIKFQQYIGW
NEKRRKGYNPNPIIAEGKHEPIIEKDLWDKVQRRMAEMSKKPQVHGKGTNVLTGLIRCSSCGHAHAAANTVNTVKGEKKK
IRYYSCSQFKNKGAAVCSANSVRADLIEPYVMNRVIEVINNDKILEKVVARANERLQEQPVQIDRNINIKKAEMNELQTK
LNNLTVALSSSPDLAPVLKDSINEYQDNITKIENEILLLTEKLKDKHIPTISVDEVKTALKAIFKDVERLEKHQLKALYL
SVIEEIHFRKHKEPGKKYEFFITLKITPEVLKEVFNDKQPEEALLSASSLFLSNNILYLEI

Nucleotide


Download         Length: 1626 bp        

>NTDB_id=560890 KFV10_RS00225 WP_254253893.1 43349..44974(-) (ccrB) [Macrococcoides canis strain IMD0709]
ATGAATAAAAAAGCAGCGATTTACGCAAGGGTTTCGACAGTAGCTCAAGCAGAAGAAGGATTTAGTATTGATGGACAAAT
TGCATTGCTGGAGGATGTTTGTAAACAAAATGGTTATGATGTTTATGACATATATGTAGACAGAGGAATCAGTGGTAAAA
ATATGGACCGTCCTGCATTATTACAGATGAATGAGGATGCACGTCAAGGAAAATTTCAAGTTATCGCAGTTTTTAAAACA
AATCGATTAGCTCGTAATACATCTGACTTATTAAAAATGGTAGAAGAATATCAGAAAAATAATGTTGAATTTTTAAGTTA
TTCGGAAAAATTTGACACGACATCAGCGGCGGGAAAATTAATGCTACAAATCATTTCCAGCATGGCAGAATTCGAACGTA
ACCAAATAGTGGAAAATGTAACAATGGGATTGGAAAAAAGAGCCAAAAATGGTTATTATAATGGAAATAAATTGTTAGGT
TATGATAAAATCCCTAATGATAAGAGACACTTAGCAATTAATGAATCAGAAGCTGAAATTGTTAGACGTATATTCAATGA
ATATGCCAATGGTAAAGGTTATCGTGCAATTGCGAATGGTCTGAACAAAGAAGGTCTTAGAACAAAGAAAGGTAATCCAT
TCGATATTGCCGGTGTGAAGTATATCTTGAATAATGTATTTTATATTGGTCAAATCAAGTTCCAACAGTACATTGGTTGG
AATGAGAAGAGACGTAAAGGCTATAATCCTAATCCTATTATCGCAGAAGGTAAGCACGAACCAATCATTGAAAAAGATTT
GTGGGATAAAGTTCAACGTCGTATGGCAGAAATGAGCAAGAAGCCTCAAGTTCATGGCAAAGGGACGAATGTATTAACTG
GCTTAATCCGTTGCAGTTCCTGTGGCCATGCACATGCTGCAGCCAATACCGTCAACACGGTTAAGGGTGAAAAGAAAAAG
ATACGTTATTACTCATGTAGTCAATTTAAAAATAAAGGTGCGGCTGTCTGTTCAGCAAATTCGGTACGAGCTGACCTTAT
CGAACCTTATGTCATGAATAGAGTCATCGAAGTAATTAATAATGATAAAATTCTAGAAAAAGTTGTGGCAAGAGCGAATG
AACGACTCCAAGAACAACCCGTACAGATAGATCGTAACATCAACATCAAAAAAGCTGAAATGAATGAATTACAAACAAAG
CTAAACAACTTAACTGTAGCTTTATCATCTTCGCCTGATTTAGCTCCCGTACTTAAAGATAGTATCAATGAATATCAGGA
TAACATCACTAAAATCGAAAACGAAATACTACTACTCACTGAAAAGCTTAAAGACAAACATATACCCACTATATCAGTTG
ATGAAGTAAAGACAGCACTGAAAGCTATTTTCAAAGACGTAGAACGTTTGGAAAAGCATCAACTTAAAGCTCTATATTTA
TCTGTCATTGAAGAAATTCACTTCCGTAAACACAAAGAACCTGGCAAGAAATATGAGTTCTTTATAACACTTAAGATCAC
ACCAGAAGTCTTGAAAGAAGTATTCAATGATAAACAACCAGAGGAAGCGCTACTCAGCGCTTCCTCTTTATTTTTGTCTA
ATAACATACTATATCTAGAGATATGA

Domains

Predicted by InterproScan.

Resolvase, N terminal domain (Resolvase)

(5-152)

Recombinase (Recombinase)

(176-277)

Recombinase zinc beta ribbon domain (Zn_ribbon_recom)

(291-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ccrB Staphylococcus aureus N315

52.865

100

0.529