Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KD909_RS10165 Genome accession   NZ_CP073755
Coordinates   1961249..1962286 (-) Length   345 a.a.
NCBI ID   WP_015881136.1    Uniprot ID   -
Organism   Exiguobacterium sp. PFWT01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1956249..1967286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KD909_RS10140 (KD909_10140) - 1956274..1956981 (-) 708 WP_074037502.1 hypothetical protein -
  KD909_RS10145 (KD909_10145) pilM 1956978..1958549 (-) 1572 WP_211781474.1 pilus assembly protein PilM -
  KD909_RS10150 (KD909_10150) - 1958562..1959314 (-) 753 WP_211781475.1 A24 family peptidase -
  KD909_RS10155 (KD909_10155) - 1959407..1959883 (-) 477 WP_211781476.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KD909_RS10160 (KD909_10160) - 1960025..1961242 (-) 1218 WP_015881137.1 type II secretion system F family protein -
  KD909_RS10165 (KD909_10165) pilT 1961249..1962286 (-) 1038 WP_015881136.1 type IV pilus twitching motility protein PilT Machinery gene
  KD909_RS10170 (KD909_10170) - 1962279..1963949 (-) 1671 WP_211781477.1 GspE/PulE family protein -
  KD909_RS10175 (KD909_10175) - 1963951..1964241 (-) 291 WP_078146549.1 hypothetical protein -
  KD909_RS10180 (KD909_10180) - 1964238..1965458 (-) 1221 WP_211781478.1 G5 domain-containing protein -
  KD909_RS10185 (KD909_10185) - 1965616..1966071 (+) 456 WP_211781479.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  KD909_RS10190 (KD909_10190) - 1966019..1966582 (+) 564 WP_249199024.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38431.24 Da        Isoelectric Point: 6.4348

>NTDB_id=560522 KD909_RS10165 WP_015881136.1 1961249..1962286(-) (pilT) [Exiguobacterium sp. PFWT01]
MINQSIETILTNAVEKRASDIHLTAGSPPIIRVDGSLITMGEEKVLPHHLEGYLKEIMTDDMRERLEHDRDLDFSFGVPR
VSRFRVNAYYQRGTIAIAFRSISGEIPTLNELNLPTVLKRLIEKPHGLILVTGPTGSGKSTTLAAMIHEINRTQRKRIIT
LEDPIEYLHSHDQSIVDQREIGSDVMKFVSGLRAALRQDPDVILLGEMRDLETISTAMTAAETGHLVMATLHTSSAMSTV
SRIIDAFPAEQQDQIRTQLANVLLGVVSQRLFPRIDVPKGRVAAMEIMLGNPAVANLIRNEKEFQLPTVIQTSRQHGMQS
MDAAIEQLVRQGIINPNAVPTEEMR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=560522 KD909_RS10165 WP_015881136.1 1961249..1962286(-) (pilT) [Exiguobacterium sp. PFWT01]
ATGATTAATCAATCGATTGAAACCATTTTGACGAATGCGGTCGAGAAACGTGCCTCGGATATTCACCTGACAGCCGGTTC
CCCACCGATTATTCGAGTAGATGGGAGTCTCATTACGATGGGCGAGGAGAAGGTATTGCCGCACCATCTTGAAGGATATT
TAAAAGAAATTATGACCGATGATATGCGCGAACGTCTCGAGCATGATCGGGATCTCGATTTTTCGTTCGGTGTCCCACGT
GTCTCTCGTTTTCGGGTCAACGCCTACTATCAACGAGGCACGATTGCCATCGCATTCCGGTCCATTTCCGGGGAAATCCC
GACATTGAACGAGCTCAATCTGCCGACGGTGCTGAAGCGTTTGATTGAGAAACCGCATGGTCTCATCTTGGTCACGGGTC
CGACGGGTTCAGGGAAATCGACGACGCTGGCCGCGATGATTCATGAAATCAATCGAACGCAGCGAAAACGGATCATCACG
CTAGAAGATCCGATTGAGTATTTGCATTCCCACGATCAAAGCATCGTCGACCAACGAGAAATCGGCTCTGACGTGATGAA
GTTCGTGAGTGGGCTAAGGGCCGCCTTACGTCAAGACCCGGACGTCATTCTACTTGGGGAGATGCGGGACTTAGAAACCA
TCTCGACGGCGATGACCGCCGCAGAGACGGGGCACCTCGTCATGGCGACGCTCCATACGTCGAGTGCGATGTCGACGGTG
AGTCGAATCATCGACGCATTCCCAGCCGAGCAACAAGACCAAATCCGGACACAACTCGCCAACGTCCTGCTTGGCGTCGT
GAGTCAACGTTTATTTCCACGGATTGATGTGCCGAAAGGCCGCGTCGCCGCCATGGAAATCATGTTGGGGAATCCGGCAG
TTGCCAACTTGATTCGAAATGAAAAAGAGTTCCAGTTGCCGACCGTCATCCAGACGAGTCGCCAACACGGGATGCAGTCG
ATGGATGCCGCCATCGAACAACTCGTTCGGCAAGGGATCATCAACCCGAACGCCGTCCCGACAGAAGAGATGAGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.76

95.362

0.484
  pilT Acinetobacter baylyi ADP1

48.788

95.652

0.467
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.181

97.681

0.461
  pilT Vibrio cholerae strain A1552

47.181

97.681

0.461
  pilT Pseudomonas aeruginosa PAK

47.879

95.652

0.458
  pilT Acinetobacter baumannii D1279779

47.024

97.391

0.458
  pilT Acinetobacter nosocomialis M2

47.024

97.391

0.458
  pilT Acinetobacter baumannii strain A118

47.024

97.391

0.458
  pilT Neisseria meningitidis 8013

47.289

96.232

0.455
  pilT Neisseria gonorrhoeae MS11

46.547

96.522

0.449
  pilT Legionella pneumophila strain Lp02

46.667

95.652

0.446
  pilT Legionella pneumophila strain ERS1305867

46.667

95.652

0.446
  pilT Pseudomonas stutzeri DSM 10701

45.946

96.522

0.443
  pilU Vibrio cholerae strain A1552

41.463

95.072

0.394
  pilU Pseudomonas stutzeri DSM 10701

40.426

95.362

0.386