Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KB879_RS02305 Genome accession   NZ_CP073677
Coordinates   444052..445533 (-) Length   493 a.a.
NCBI ID   WP_153953205.1    Uniprot ID   -
Organism   Cupriavidus sp. KK10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 439052..450533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KB879_RS02275 (KB879_02275) - 439382..440041 (-) 660 WP_013955470.1 LON peptidase substrate-binding domain-containing protein -
  KB879_RS02280 (KB879_02280) rapZ 440133..441017 (-) 885 WP_013955471.1 RNase adapter RapZ -
  KB879_RS02285 (KB879_02285) - 441135..441527 (-) 393 WP_010814383.1 PsiF family protein -
  KB879_RS02290 (KB879_02290) hprK 441690..442661 (-) 972 WP_010814384.1 HPr(Ser) kinase/phosphatase -
  KB879_RS02295 (KB879_02295) ptsN 442742..443197 (-) 456 WP_010814385.1 PTS IIA-like nitrogen regulatory protein PtsN -
  KB879_RS02300 (KB879_02300) hpf 443653..444003 (-) 351 WP_010814386.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  KB879_RS02305 (KB879_02305) rpoN/rpoN1 444052..445533 (-) 1482 WP_153953205.1 RNA polymerase factor sigma-54 Machinery gene
  KB879_RS02310 (KB879_02310) lptB 445669..446469 (-) 801 WP_013955473.1 LPS export ABC transporter ATP-binding protein -
  KB879_RS02315 (KB879_02315) lptA 446527..447177 (-) 651 WP_013955474.1 lipopolysaccharide transport periplasmic protein LptA -
  KB879_RS02320 (KB879_02320) lptC 447246..447875 (-) 630 WP_013955475.1 LPS export ABC transporter periplasmic protein LptC -
  KB879_RS02325 (KB879_02325) - 447919..448902 (-) 984 WP_035822810.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54682.94 Da        Isoelectric Point: 4.8616

>NTDB_id=559863 KB879_RS02305 WP_153953205.1 444052..445533(-) (rpoN/rpoN1) [Cupriavidus sp. KK10]
MKPSLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVEQALTENPLLERENDWIESPLRVAADGSVNLQSAPAPAPAEPQ
GNGEARADGAGDDDSYGDSGNGDDYGSGDWSLDDFARRPQGDEDEKTPMQLREAEPTLREYLMEQLTPLKISARDKGLAI
FLIESLDDDGYLSASLEEICTELPEELEFEIDEVHAILTLLQSFDPPGVGARNAAECLALQLRRLTHPQRELALNIVSNH
LELLAVRDYTRLKKALQVDEVALKSAHELIRSLAPYPGHAYSRPEADFVVPDVFVRKGGGGWIAQLNPDVMPRLRINDMY
AQILRGAKGESGTAGLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKNFFSHGEIAMRPLVLREIADTLGLHESTISR
VTTNKFMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLIGAEDPRNPLSDSRIAELLGEQGFVVARRTVAKYRE
ALKIPAVNLRKSL

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=559863 KB879_RS02305 WP_153953205.1 444052..445533(-) (rpoN/rpoN1) [Cupriavidus sp. KK10]
ATGAAACCGTCGCTACAGCTCCGTCTTTCCCAGCATCTGGCCCTGACCCCGCAACTGCAGCAGTCGATCCGGCTGCTGCA
GCTCTCCACGCTGGAACTGCAGCAAGAGGTCGAACAGGCGCTGACGGAAAACCCGCTGCTCGAGCGCGAGAACGACTGGA
TCGAAAGCCCGCTGCGCGTGGCGGCCGACGGCTCGGTCAACCTGCAGAGCGCACCGGCGCCCGCCCCGGCTGAGCCCCAG
GGCAATGGCGAGGCTCGCGCCGACGGCGCGGGTGACGACGACAGCTATGGCGACAGCGGCAACGGCGACGACTACGGCAG
CGGCGACTGGAGCCTGGACGACTTTGCCCGCCGCCCCCAGGGCGACGAGGATGAAAAAACGCCGATGCAGCTGCGCGAGG
CCGAGCCCACGCTGCGCGAGTACCTGATGGAGCAGCTCACGCCGCTGAAGATCTCGGCGCGCGACAAAGGCCTGGCCATC
TTCCTGATCGAATCGCTCGACGACGATGGCTACCTGAGCGCATCGCTTGAGGAGATCTGCACGGAGTTGCCGGAAGAACT
CGAGTTCGAGATCGACGAAGTCCACGCCATCCTCACGCTGCTGCAGAGTTTCGACCCGCCCGGCGTGGGTGCGCGCAACG
CCGCCGAGTGCCTGGCCCTGCAGTTGCGACGCCTGACGCACCCGCAACGCGAACTGGCGCTGAACATCGTTAGCAATCAC
CTGGAGTTGCTCGCAGTACGCGACTACACGCGGCTGAAGAAGGCGCTGCAGGTAGATGAAGTGGCACTGAAGTCCGCACA
CGAACTGATCCGCTCGCTGGCGCCCTACCCCGGCCATGCGTACAGCCGCCCGGAAGCGGACTTTGTGGTGCCGGACGTGT
TCGTGCGCAAGGGTGGCGGCGGCTGGATCGCGCAGCTCAATCCGGATGTGATGCCGAGGCTGCGCATCAACGATATGTAT
GCCCAAATCCTGCGTGGCGCGAAGGGTGAGTCCGGCACGGCCGGACTGCAGCAAAAGCTGCAAGAGGCGCGCTGGCTGAT
CAAGAACATCCAGCAAAGGTTCGACACAATCCTGCGTGTCTCGCAGGCCATTGTCGAGCGTCAAAAGAACTTTTTCAGTC
ACGGCGAAATCGCCATGCGCCCCTTGGTTTTGCGGGAGATTGCCGATACACTGGGTTTACACGAGTCAACCATCTCCCGG
GTGACGACCAATAAATTCATGGCAACGCCGATGGGTACTTTCGAACTGAAGTACTTCTTCGGCAGCCACGTGTCCACCGA
AACCGGTGGCGCGGCTTCATCAACGGCCATCCGCGCCTTGATCAAGCAACTGATAGGAGCCGAAGACCCGAGGAATCCCC
TTTCCGACAGTCGCATTGCCGAACTGCTGGGTGAGCAAGGCTTTGTTGTTGCGCGCCGCACTGTTGCCAAGTATCGCGAA
GCCCTGAAGATCCCCGCAGTCAATCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-320)

Sigma-54, DNA binding domain (Sigma54_DBD)

(336-491)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

79.242

100

0.805