Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KEH56_RS26425 Genome accession   NZ_CP073673
Coordinates   1541801..1543306 (+) Length   501 a.a.
NCBI ID   WP_006483132.1    Uniprot ID   -
Organism   Burkholderia cenocepacia strain toggle2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1536801..1548306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEH56_RS26400 (KEH56_26400) kdsD 1538032..1539015 (+) 984 WP_006483136.1 arabinose 5-phosphate isomerase KdsD -
  KEH56_RS26405 (KEH56_26405) - 1539015..1539551 (+) 537 WP_212203248.1 HAD family hydrolase -
  KEH56_RS26410 (KEH56_26410) lptC 1539555..1540169 (+) 615 WP_006483145.1 LPS export ABC transporter periplasmic protein LptC -
  KEH56_RS26415 (KEH56_26415) lptA 1540190..1540855 (+) 666 WP_006483139.1 lipopolysaccharide transport periplasmic protein LptA -
  KEH56_RS26420 (KEH56_26420) lptB 1540852..1541628 (+) 777 WP_006483143.1 LPS export ABC transporter ATP-binding protein -
  KEH56_RS26425 (KEH56_26425) rpoN/rpoN1 1541801..1543306 (+) 1506 WP_006483132.1 RNA polymerase factor sigma-54 Machinery gene
  KEH56_RS26430 (KEH56_26430) raiA 1543465..1543824 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  KEH56_RS26435 (KEH56_26435) - 1544020..1544532 (+) 513 WP_034195643.1 PTS sugar transporter subunit IIA -
  KEH56_RS26440 (KEH56_26440) hprK 1544662..1545630 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  KEH56_RS26445 (KEH56_26445) rapZ 1545705..1546613 (+) 909 WP_006483144.1 RNase adapter RapZ -
  KEH56_RS26450 (KEH56_26450) - 1546640..1547275 (+) 636 WP_006499012.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54735.87 Da        Isoelectric Point: 4.8740

>NTDB_id=559842 KEH56_RS26425 WP_006483132.1 1541801..1543306(+) (rpoN/rpoN1) [Burkholderia cenocepacia strain toggle2]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNGSSPSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLAAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=559842 KEH56_RS26425 WP_006483132.1 1541801..1543306(+) (rpoN/rpoN1) [Burkholderia cenocepacia strain toggle2]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCACAGAACACCGAGCCCGCCGCG
ACCAACGGCAGCAGCCCGTCCAGCGATCGCGCGGAGCGCGACGAACCGGCCGGCGCCGACGAATACGACGGCTACGGGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATACGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTCGCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAACTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGCGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAGCTGCTGCGTCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCACCTCGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCACTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAGCTCAATCCGGAAGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGCTGAAACAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689