Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KEC55_RS14650 Genome accession   NZ_CP073637
Coordinates   3145939..3147444 (+) Length   501 a.a.
NCBI ID   WP_282505996.1    Uniprot ID   -
Organism   Burkholderia cepacia strain ZJ1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3140939..3152444
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEC55_RS14625 (KEC55_14600) kdsD 3142172..3143155 (+) 984 WP_282505994.1 arabinose 5-phosphate isomerase KdsD -
  KEC55_RS14630 (KEC55_14605) - 3143155..3143691 (+) 537 WP_176049647.1 HAD family hydrolase -
  KEC55_RS14635 (KEC55_14610) lptC 3143695..3144309 (+) 615 WP_176049646.1 LPS export ABC transporter periplasmic protein LptC -
  KEC55_RS14640 (KEC55_14615) lptA 3144330..3144995 (+) 666 WP_282505995.1 lipopolysaccharide transport periplasmic protein LptA -
  KEC55_RS14645 (KEC55_14620) lptB 3144992..3145768 (+) 777 WP_059233172.1 LPS export ABC transporter ATP-binding protein -
  KEC55_RS14650 (KEC55_14625) rpoN/rpoN1 3145939..3147444 (+) 1506 WP_282505996.1 RNA polymerase factor sigma-54 Machinery gene
  KEC55_RS14655 (KEC55_14630) raiA 3147603..3147962 (+) 360 WP_006477786.1 ribosome-associated translation inhibitor RaiA -
  KEC55_RS14660 (KEC55_14635) - 3148158..3148670 (+) 513 WP_034195643.1 PTS sugar transporter subunit IIA -
  KEC55_RS14665 (KEC55_14640) hprK 3148800..3149768 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  KEC55_RS14670 (KEC55_14645) rapZ 3149844..3150752 (+) 909 WP_282505997.1 RNase adapter RapZ -
  KEC55_RS14675 (KEC55_14650) - 3150780..3151415 (+) 636 WP_176049642.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54816.90 Da        Isoelectric Point: 4.9328

>NTDB_id=559671 KEC55_RS14650 WP_282505996.1 3145939..3147444(+) (rpoN/rpoN1) [Burkholderia cepacia strain ZJ1]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAS
SNGSSTSSDRTERDERGGADDYDGYGSGDGNDGPQWNLDEFGRASGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLTDLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=559671 KEC55_RS14650 WP_282505996.1 3145939..3147444(+) (rpoN/rpoN1) [Burkholderia cepacia strain ZJ1]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCGCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCGCAGAACACCGAGCCGGCCTCG
TCAAACGGCAGCAGCACGTCCAGTGATCGTACGGAGCGCGACGAGCGAGGCGGCGCCGACGATTACGACGGTTATGGGTC
CGGCGACGGCAACGACGGCCCGCAATGGAATCTCGACGAGTTCGGCCGCGCGAGCGGCGCGTCCGACGACGACGATCTCC
CACCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGTGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGACGCTCGACGAAGTGCT
CACCGACCTGCCGCCCGAGCTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCACTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGCGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAGCTGCTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGATTTCACACGGTTGCGCAAGCACCTGAAGGCAAG
CGACGACGCGCTGCGCGACGCGCATGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCGTACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGGGATCCGTCGGCCGGTTCGCTGAAGCAACA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCTTCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGATAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGGTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCCGTCAATCTGCGAAAGTCTCTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.787

100

0.685