Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   KEF49_RS12080 Genome accession   NZ_CP073635
Coordinates   2407816..2408583 (-) Length   255 a.a.
NCBI ID   WP_014470678.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Ba13     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2402816..2413583
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEF49_RS12060 (KEF49_12060) - 2403017..2403829 (-) 813 WP_013352914.1 pyruvate, water dikinase regulatory protein -
  KEF49_RS12065 (KEF49_12065) ccpN 2403855..2404493 (-) 639 WP_014470676.1 transcriptional regulator CcpN -
  KEF49_RS12070 (KEF49_12070) glyS 2404594..2406633 (-) 2040 WP_212098494.1 glycine--tRNA ligase subunit beta -
  KEF49_RS12075 (KEF49_12075) glyQ 2406626..2407513 (-) 888 WP_013352917.1 glycine--tRNA ligase subunit alpha -
  KEF49_RS12080 (KEF49_12080) recO 2407816..2408583 (-) 768 WP_014470678.1 DNA repair protein RecO Machinery gene
  KEF49_RS12085 (KEF49_12085) - 2408620..2408763 (-) 144 WP_007408286.1 YqzL family protein -
  KEF49_RS12090 (KEF49_12090) era 2408918..2409823 (-) 906 WP_007408285.1 GTPase Era -
  KEF49_RS12095 (KEF49_12095) - 2409804..2410214 (-) 411 WP_045510514.1 cytidine deaminase -
  KEF49_RS12100 (KEF49_12100) - 2410337..2410711 (-) 375 WP_065981906.1 diacylglycerol kinase family protein -
  KEF49_RS12105 (KEF49_12105) ybeY 2410692..2411165 (-) 474 WP_013352922.1 rRNA maturation RNase YbeY -
  KEF49_RS12110 (KEF49_12110) - 2411166..2413298 (-) 2133 WP_088613137.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29300.98 Da        Isoelectric Point: 9.0137

>NTDB_id=559600 KEF49_RS12080 WP_014470678.1 2407816..2408583(-) (recO) [Bacillus amyloliquefaciens strain Ba13]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSRDG
TFHFSVRDNGFICHRCFGKDPYKVPISPQTARLLRLFYYFDIGRLGSVSLKQETKNELKRVIDLYYEEYSGLYLKSKRFL
DQMESMKHLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=559600 KEF49_RS12080 WP_014470678.1 2407816..2408583(-) (recO) [Bacillus amyloliquefaciens strain Ba13]
ATGCTGACAAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGAGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTTATGGCAAGAGGCGCCAGGAAGTCAGCCAGCCGGCTCTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGACTTGTTTTTAACGGCATATGCCGCTTTTATCGCTGAACTTACAGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGAAGGGACTGACCCGGACGTGATCACCTTCA
TCGTTCAGATGAAAATGCTCGGCGTCATGGGACTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCCGGGACGGG
ACGTTTCATTTTTCGGTAAGAGATAACGGTTTTATCTGCCACCGCTGCTTTGGAAAGGATCCGTACAAAGTTCCGATTTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGTAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CGAAAAACGAACTTAAACGGGTTATCGATCTGTATTATGAGGAATATTCAGGGCTTTATTTAAAGTCTAAACGGTTTTTA
GACCAGATGGAAAGCATGAAGCACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

90.196

100

0.902