Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   KEF49_RS08330 Genome accession   NZ_CP073635
Coordinates   1721123..1721494 (+) Length   123 a.a.
NCBI ID   WP_249199327.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain Ba13     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1716123..1726494
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEF49_RS08325 (KEF49_08325) - 1719559..1720770 (-) 1212 WP_212101423.1 cytochrome P450 -
  KEF49_RS08330 (KEF49_08330) nucA/comI 1721123..1721494 (+) 372 WP_249199327.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  KEF49_RS08335 (KEF49_08335) - 1721544..1722299 (+) 756 WP_212101429.1 YoaK family protein -
  KEF49_RS08340 (KEF49_08340) - 1722333..1722695 (-) 363 WP_065981515.1 hypothetical protein -
  KEF49_RS08345 (KEF49_08345) - 1722891..1724219 (-) 1329 WP_115997271.1 S8 family peptidase -
  KEF49_RS08350 (KEF49_08350) - 1724403..1724636 (+) 234 WP_014471844.1 hypothetical protein -
  KEF49_RS08355 (KEF49_08355) - 1724891..1725598 (+) 708 WP_212101432.1 poly-gamma-glutamate hydrolase family protein -
  KEF49_RS08360 (KEF49_08360) - 1725659..1726111 (+) 453 WP_212101435.1 OsmC family protein -
  KEF49_RS08365 (KEF49_08365) - 1726125..1726478 (-) 354 WP_249199304.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 123 a.a.        Molecular weight: 13359.80 Da        Isoelectric Point: 5.5777

>NTDB_id=559588 KEF49_RS08330 WP_249199327.1 1721123..1721494(+) (nucA/comI) [Bacillus amyloliquefaciens strain Ba13]
MDAGIHSENTAEAASRYDQVLYFPLLKYPETGNHIKDAISAGHSDICTIDRGGAEHRRKESLKGIPTKPGFDRDEWPMAV
CTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 372 bp        

>NTDB_id=559588 KEF49_RS08330 WP_249199327.1 1721123..1721494(+) (nucA/comI) [Bacillus amyloliquefaciens strain Ba13]
ATGGACGCCGGCATCCATTCGGAGAATACTGCTGAAGCTGCGTCCCGATACGATCAGGTGCTGTATTTCCCGCTGTTAAA
ATATCCGGAGACGGGAAATCATATAAAAGACGCCATTTCGGCAGGGCATTCTGACATTTGTACAATTGATCGCGGCGGAG
CTGAGCATAGAAGGAAAGAATCATTGAAAGGCATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCGATGGCAGTC
TGTACGGAAGGCGGGGCAGGGGCTGACATCAGATATGTAACCCCCTCAGATAATCGCGGAGCCGGTTCATGGGTTGGAAA
TCAAATGAGCGGATATTCCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.617

86.992

0.545