Detailed information NTG0559572

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: KEF49_RS05165
• Genome accession: NZ_CP073635
• Coordinates: 1049788..1053492 (+)
• Length: 1234 a.a.
• NCBI ID: WP_212101650.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain Ba13
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1044788..1058492

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KEF49_RS05150 (KEF49_05150)	-	1044848..1045375 (-)	528	WP_088462317.1	ferritin-like domain-containing protein	-
KEF49_RS05155 (KEF49_05155)	-	1045446..1046159 (-)	714	WP_115997926.1	DUF421 domain-containing protein	-
KEF49_RS05160 (KEF49_05160)	addB	1046301..1049801 (+)	3501	WP_115997927.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
KEF49_RS05165 (KEF49_05165)	addA	1049788..1053492 (+)	3705	WP_212101650.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
KEF49_RS05170 (KEF49_05170)	sbcD	1053559..1054731 (+)	1173	WP_065981123.1	exonuclease subunit SbcD	-
KEF49_RS05175 (KEF49_05175)	sbcC	1054728..1058120 (+)	3393	WP_212100866.1	exonuclease subunit SbcC	-
KEF49_RS05180 (KEF49_05180)	-	1058136..1058435 (+)	300	WP_065981125.1	hypothetical protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 141279.86 Da
• Isoelectric Point: 5.2550

>NTDB_id=559572 KEF49_RS05165 WP_212101650.1 1049788..1053492(+) (addA) [Bacillus amyloliquefaciens strain Ba13]
MQIPKPKDSIWTDDQWSAIVSSGRDILVAAAAGSGKTAVLVERMIRKITAEEEPVDVDRLLVVTFTNASAAEMKHRIAEA
LEKELAKNPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYMIDLDPGFRMADQTEGELLGDEVLDELFEDEYAKGNQA
FFELVDRYTTDRHDLDLQDLVKRVYEYSRSHPDPEAWLKSFVRLYDVTEESKMEELPFYEYVREDAEMALFGAKQKLDKA
LELTKAPGGPAPRADNILDDLQQIEELISCRHDFKALYERVPTVTFKRAKAVKGDEFDKALLDEATDLRNGAKKLIEKLK
TDYFTRSPQDHLKSLKDMKPVIETLVQLVISYGKRFEAAKQEKSIIDFSDLEHYCLAILTARDEEGRRVPSEAAVYYQDQ
FHEVLVDEYQDTNLVQESILQLVKSGNEESGNLFMVGDVKQSIYRFRLAEPLLFLGKYKRFTENGEGAGQKIDLNQNFRS
RSDILDSTNFLFKQLMGGKIGEVDYDEQAALKLGASYPPNDAAKTELLLIDSADDADSPEDAEDFETVHWEAKAIAAEIR
KLVSSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQLMEELKNQGIPVYANLTSGYFEAVEVAAALSVLKVIDNPYQDI
PLASVLRSPIVGCDENELALIRLEKKKAPFYEALKAYIANGDRHDDLYQKLRTFYDSLQKWRSFSTNHSVSELIWEVYRD
TGYFDYAGGMPGGKQRQANLRVLYDRARSYEATAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRSFNMMDLNKSYLLDKELGFGTKYIHPELRISYPTLPLVAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKNREKQLAKWQAQADRADWLLSEFDRYQASSYLDFIGPALIRHRDMEAHRAPGLLAPEDIAHDPARFHIRMLQQSE
LLEDSPEERAEEKSKRLKAIQHGEPIPDSFSFDDQARRLLEWEYPYRKLTAIRTKQSVSELKRKQEYEDEYSGRSLIKPS
GDTLLYKRPGFMMKKGLTAAEKGTAMHTVMQHIPLTHAPTAEEAERTVRMLYEKELLTEEQLDAIDIDELIQFFGTEIGK
DLVGALRIDREVPFSMALPASEVYKDAETAGEPLLVQGIIDCLYETADGLYLLDYKTDRIEGKFRNGFEGAAPILQKRYE
TQIELYTKAVEQITKTKVKGRALYFFDGGHVLTL

Nucleotide

• Length: 3705 bp

>NTDB_id=559572 KEF49_RS05165 WP_212101650.1 1049788..1053492(+) (addA) [Bacillus amyloliquefaciens strain Ba13]
ATGCAAATTCCTAAACCGAAAGACAGCATATGGACGGATGACCAATGGAGCGCCATCGTTTCTTCCGGCCGCGATATTCT
GGTCGCTGCCGCAGCCGGATCAGGTAAAACGGCCGTGCTTGTAGAGCGGATGATCAGAAAGATAACCGCTGAAGAAGAGC
CGGTTGATGTTGACAGGCTCCTTGTGGTGACATTTACAAACGCCTCAGCAGCGGAAATGAAGCATCGGATTGCTGAAGCA
TTAGAAAAAGAACTCGCCAAAAATCCGGGGTCTCTGCATATCAGACGGCAGCTGTCTCTATTAAATCGGGCGAGCATTTC
GACGCTTCACTCTTTCTGTCTGCAGGTGCTTAAAAAATATTATTATATGATTGATCTTGACCCGGGTTTTCGGATGGCTG
ACCAGACGGAAGGCGAATTGCTCGGAGACGAAGTGCTTGATGAGCTGTTTGAGGATGAATACGCAAAAGGGAATCAGGCG
TTTTTTGAGCTTGTGGACAGATATACGACGGACAGGCACGATCTGGATCTTCAAGACCTCGTCAAGCGGGTGTATGAGTA
TTCCCGGTCGCATCCTGACCCGGAAGCGTGGCTGAAAAGCTTTGTCCGCCTATATGATGTGACAGAAGAAAGCAAAATGG
AAGAGCTGCCGTTTTACGAGTATGTGAGAGAAGATGCTGAAATGGCGCTCTTCGGGGCAAAACAAAAGCTAGATAAGGCG
CTTGAACTGACAAAAGCGCCCGGCGGGCCGGCCCCGCGTGCTGATAATATTCTGGATGACCTTCAGCAGATAGAAGAGCT
GATCAGCTGCCGGCATGACTTTAAGGCGCTGTATGAGCGGGTTCCTACGGTCACATTTAAACGGGCCAAGGCGGTAAAGG
GTGATGAATTTGACAAGGCGCTTTTGGATGAAGCGACTGATTTGCGAAACGGCGCGAAGAAACTGATCGAAAAGTTAAAA
ACCGATTACTTTACAAGAAGCCCGCAAGATCATTTAAAGAGTTTAAAAGACATGAAGCCTGTCATTGAAACCCTCGTTCA
GCTCGTAATATCGTACGGAAAACGATTTGAAGCGGCGAAACAAGAAAAATCAATCATTGATTTCTCAGATTTGGAACATT
ACTGCCTGGCGATTTTAACTGCGAGGGATGAAGAAGGCCGGCGTGTGCCGAGTGAAGCGGCCGTTTATTATCAAGACCAG
TTCCATGAAGTTCTGGTTGATGAATATCAAGATACCAATCTTGTGCAGGAATCCATTTTGCAGCTTGTTAAAAGCGGGAA
TGAAGAGTCGGGAAATCTGTTTATGGTAGGGGACGTCAAGCAATCAATCTACCGTTTCAGACTTGCCGAGCCGTTGCTTT
TTCTCGGGAAATACAAAAGGTTTACGGAAAATGGGGAAGGCGCCGGGCAGAAAATCGACCTGAACCAAAACTTCCGAAGC
CGCTCCGATATTTTGGACAGTACGAATTTCTTGTTTAAGCAGCTGATGGGCGGCAAAATCGGCGAAGTGGACTACGATGA
ACAGGCGGCATTAAAGCTTGGCGCATCATATCCGCCGAATGACGCGGCGAAAACGGAACTGCTGCTGATTGACAGCGCTG
ATGACGCAGACAGCCCCGAGGATGCCGAAGACTTTGAAACCGTGCATTGGGAAGCGAAAGCGATAGCCGCGGAGATCCGG
AAGCTTGTCTCATCTCCGTTTAAGGTATATGACGGCAAAACAAAAACACACCGGAATATCCAGTACCGGGATATCGTGAT
TTTGCTGCGCTCCATGCCATGGGCTCCGCAATTAATGGAAGAGCTGAAAAACCAAGGCATTCCGGTTTACGCCAATTTGA
CGTCAGGTTACTTTGAAGCCGTGGAGGTGGCTGCGGCGCTTTCAGTCTTAAAGGTGATTGATAATCCGTATCAGGATATT
CCGCTAGCTTCCGTGCTGCGCTCGCCCATCGTCGGCTGTGACGAAAATGAACTCGCGCTCATACGCCTTGAAAAGAAAAA
AGCGCCGTTTTATGAAGCGCTGAAAGCGTATATAGCGAACGGTGACAGACATGACGACCTGTATCAAAAACTGAGAACAT
TTTATGACAGTCTGCAGAAGTGGCGGTCATTCTCGACGAATCATTCAGTGTCTGAGCTGATTTGGGAAGTATACCGCGAT
ACCGGATATTTTGATTATGCAGGCGGCATGCCTGGAGGAAAGCAGCGTCAGGCCAATCTGCGCGTCTTGTATGACCGGGC
GCGTTCGTATGAAGCGACGGCATTCCGCGGGTTATTCCGCTTTTTGCGGTTCATTGAACGGATGCAGGAGCGCGGCGATG
ATTTGGGGACGGCTCGGGCGCTCAGCGAGCAGGAGGATGTCGTTCGGCTGATGACGATCCACAGCAGCAAAGGACTTGAG
TTCCCCGTCGTTTTCACCGCCGGACTTGGCAGAAGTTTTAATATGATGGATCTCAACAAATCATACCTGCTTGATAAAGA
GCTCGGTTTCGGCACGAAGTATATTCATCCGGAACTTAGAATCAGCTATCCGACTCTGCCGCTTGTGGCAATGAAGAAAA
AAATGCGCAGAGAGCTTTTATCAGAAGAATTGCGCGTATTGTATGTGGCGCTGACAAGGGCGAAGGAAAAGCTGTTTCTC
GTCGGTTCCTGCAAAAACCGGGAAAAACAGCTGGCCAAATGGCAGGCGCAAGCAGACCGGGCTGATTGGCTCCTTTCTGA
ATTCGACCGCTATCAGGCGTCATCTTATTTGGACTTTATCGGTCCGGCGCTCATCCGTCACCGTGACATGGAGGCGCACA
GGGCTCCGGGGCTTTTGGCGCCGGAGGATATCGCGCATGACCCGGCGCGTTTTCACATCCGGATGCTGCAGCAAAGTGAA
TTGCTGGAAGATAGTCCTGAGGAACGTGCTGAAGAAAAGAGCAAACGTTTAAAAGCGATACAGCATGGCGAGCCGATTCC
TGATTCGTTTTCATTTGATGATCAGGCGCGCCGTCTTCTTGAATGGGAATATCCGTATCGGAAGCTGACCGCCATCAGAA
CGAAGCAGTCTGTTTCTGAGCTGAAGAGAAAGCAAGAATACGAGGATGAGTACAGCGGACGCTCTCTCATCAAACCGTCA
GGGGACACTCTTTTATACAAGCGTCCCGGCTTTATGATGAAAAAAGGGCTGACAGCCGCCGAAAAAGGGACGGCGATGCA
TACGGTGATGCAGCACATACCGCTGACGCATGCTCCGACTGCTGAAGAAGCGGAGCGGACGGTCCGGATGCTTTACGAAA
AAGAGCTGCTGACAGAAGAACAGCTGGACGCAATTGATATCGATGAGCTGATTCAGTTTTTCGGAACTGAAATCGGAAAA
GACCTTGTTGGAGCGCTGCGGATTGATCGGGAAGTGCCGTTCAGTATGGCGCTTCCGGCCAGCGAGGTGTACAAGGATGC
CGAAACGGCCGGTGAACCGCTGCTCGTCCAGGGAATTATTGACTGCCTCTACGAAACTGCGGACGGACTCTATTTGCTGG
ACTATAAAACGGATCGGATCGAGGGGAAATTCCGGAACGGATTTGAAGGCGCGGCCCCGATCCTGCAAAAAAGGTATGAA
ACACAAATCGAATTGTATACAAAAGCGGTTGAACAAATTACAAAAACGAAAGTGAAAGGGCGTGCGCTCTATTTCTTCGA
TGGAGGTCACGTTCTTACACTGTAA

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1055-1226)

• UvrD-like helicase C-terminal domain (UvrD_C) (472-881)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	82.172	100	0.822