Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KEM15_RS00680 Genome accession   NZ_CP073632
Coordinates   129941..131308 (+) Length   455 a.a.
NCBI ID   WP_274504901.1    Uniprot ID   -
Organism   Streptococcus parasuis strain NN1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 130115..162937 129941..131308 flank -1193


Gene organization within MGE regions


Location: 129941..162937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEM15_RS00680 (KEM15_00665) radA 129941..131308 (+) 1368 WP_274504901.1 DNA repair protein RadA Machinery gene
  KEM15_RS00685 (KEM15_00670) - 131431..131925 (+) 495 WP_024391588.1 carbonic anhydrase -
  KEM15_RS00690 (KEM15_00675) - 132125..132883 (+) 759 WP_217374528.1 TIGR00266 family protein -
  KEM15_RS00695 - 133054..133179 (+) 126 WP_274504461.1 hypothetical protein -
  KEM15_RS00700 (KEM15_00680) - 133166..133537 (+) 372 WP_274504462.1 hypothetical protein -
  KEM15_RS00705 (KEM15_00685) gltX 133927..135381 (+) 1455 WP_130554215.1 glutamate--tRNA ligase -
  KEM15_RS00710 (KEM15_00690) - 135520..136896 (+) 1377 WP_274504463.1 amino acid permease -
  KEM15_RS00715 (KEM15_00695) mmuM 136909..137856 (+) 948 WP_274504464.1 homocysteine S-methyltransferase -
  KEM15_RS00720 (KEM15_00700) - 137893..138255 (-) 363 WP_274504465.1 MerR family transcriptional regulator -
  KEM15_RS00725 (KEM15_00705) - 138317..138607 (+) 291 WP_274504466.1 hypothetical protein -
  KEM15_RS00730 (KEM15_00710) - 138604..138957 (-) 354 WP_274504467.1 NUDIX domain-containing protein -
  KEM15_RS10235 (KEM15_00715) - 139226..139967 (+) 742 Protein_123 VOC family protein -
  KEM15_RS00745 (KEM15_00720) - 140071..140598 (+) 528 WP_024397302.1 transcription repressor NadR -
  KEM15_RS00750 (KEM15_00725) - 140669..141217 (+) 549 WP_024403818.1 hypothetical protein -
  KEM15_RS00755 (KEM15_00730) - 141408..142601 (+) 1194 WP_274504470.1 argininosuccinate synthase -
  KEM15_RS00760 (KEM15_00735) argH 142615..144009 (+) 1395 WP_274504471.1 argininosuccinate lyase -
  KEM15_RS00765 (KEM15_00740) rnpA 144080..144439 (+) 360 WP_024391601.1 ribonuclease P protein component -
  KEM15_RS00770 (KEM15_00745) - 144423..145235 (+) 813 WP_217374536.1 YidC/Oxa1 family membrane protein insertase -
  KEM15_RS00775 (KEM15_00750) jag 145254..146258 (+) 1005 WP_172036507.1 RNA-binding cell elongation regulator Jag/EloR -
  KEM15_RS00780 (KEM15_00755) rpmH 146479..146616 (+) 138 WP_049618373.1 50S ribosomal protein L34 -
  KEM15_RS00785 (KEM15_00760) - 146666..147547 (-) 882 WP_274504472.1 diacylglycerol kinase family protein -
  KEM15_RS00790 (KEM15_00765) - 147697..148479 (+) 783 WP_217374539.1 TatD family hydrolase -
  KEM15_RS00795 (KEM15_00770) rnmV 148463..149029 (+) 567 WP_274504473.1 ribonuclease M5 -
  KEM15_RS00800 (KEM15_00775) - 149058..149308 (+) 251 Protein_135 NUDIX domain-containing protein -
  KEM15_RS00805 (KEM15_00780) rsmA 149347..150222 (+) 876 WP_217374541.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
  KEM15_RS00810 (KEM15_00785) - 150219..150455 (+) 237 WP_274504474.1 hypothetical protein -
  KEM15_RS00815 (KEM15_00790) - 150585..151130 (-) 546 WP_274504475.1 cysteine hydrolase family protein -
  KEM15_RS00820 (KEM15_00795) - 151722..153458 (+) 1737 WP_274504476.1 hypothetical protein -
  KEM15_RS00830 (KEM15_00805) rsgA 153812..154687 (+) 876 WP_172036513.1 ribosome small subunit-dependent GTPase A -
  KEM15_RS00835 (KEM15_00810) rpe 154702..155361 (+) 660 WP_130554196.1 ribulose-phosphate 3-epimerase -
  KEM15_RS00840 (KEM15_00815) - 155354..155986 (+) 633 WP_274504477.1 thiamine diphosphokinase -
  KEM15_RS00845 (KEM15_00820) rmuC 155987..157225 (+) 1239 WP_172041247.1 DNA recombination protein RmuC -
  KEM15_RS00850 (KEM15_00825) - 157215..158156 (+) 942 WP_130554193.1 3'-5' exoribonuclease YhaM family protein -
  KEM15_RS00855 (KEM15_00830) - 158156..159022 (+) 867 WP_274504478.1 phosphotransferase -
  KEM15_RS00860 (KEM15_00835) purR 159125..159937 (+) 813 WP_024391625.1 pur operon repressor -
  KEM15_RS00865 (KEM15_00840) - 159991..160545 (+) 555 WP_274504479.1 hypothetical protein -
  KEM15_RS00870 (KEM15_00845) - 160591..160767 (+) 177 WP_165390448.1 hypothetical protein -
  KEM15_RS00875 (KEM15_00850) - 160796..160951 (+) 156 WP_217374550.1 hypothetical protein -
  KEM15_RS00880 (KEM15_00855) - 161104..161967 (-) 864 WP_274504480.1 RluA family pseudouridine synthase -
  KEM15_RS00885 (KEM15_00860) - 161969..162937 (-) 969 WP_274504481.1 NAD(P)/FAD-dependent oxidoreductase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49854.35 Da        Isoelectric Point: 6.1911

>NTDB_id=559496 KEM15_RS00680 WP_274504901.1 129941..131308(+) (radA) [Streptococcus parasuis strain NN1]
MIIAKKKTTFVCQNCEYHSPKYLGRCPNCGSWSSFVEEVEVAEVKNERVSLTGEKTRPMKLNEVSSIQVARTKTNMEEFN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSTIGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSIRTE
IEKIKPDFLIIDSIQTIMSPDISSVQGSVSQVREVTNELMQIAKTNNIATFIVGHMTKEGTLAGPRTLEHMVDTVLYFEG
ERQHTFRILRAVKNRFGSTNEIGIFEMQSQGLVEVLNPSEVFLEERLDGATGSAIVVTMEGTRPILAEVQALVTPSMFGN
AKRTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVALASSYKDKPTNPQECFIGEIGLTGE
IRRVNRIEQRINEAAKLGFTKVYAPKNSLTGIKVPKEITVIGVATIGEVLQKVFN

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=559496 KEM15_RS00680 WP_274504901.1 129941..131308(+) (radA) [Streptococcus parasuis strain NN1]
ATCATCATCGCTAAGAAAAAAACAACCTTTGTCTGCCAAAATTGTGAATACCATTCGCCCAAGTATCTGGGCCGTTGCCC
CAACTGTGGCTCCTGGTCTAGCTTTGTCGAGGAAGTTGAAGTCGCTGAAGTCAAGAACGAGCGGGTTAGCCTGACAGGTG
AGAAGACCCGTCCCATGAAGCTCAATGAAGTTTCCTCCATTCAAGTGGCTCGCACTAAGACCAACATGGAGGAGTTCAAC
CGCGTCCTCGGTGGCGGTGTGGTGCCAGGAAGTCTGGTCTTGATCGGAGGCGATCCAGGGATTGGCAAATCCACCCTACT
CTTGCAGGTTTCGACTCAGCTTTCGACCATTGGTACCGTCCTCTATGTGTCCGGGGAAGAGTCTGCCCAGCAGATTAAGT
TGCGGGCGGAACGTTTGGGCGACATCGACAGCGAGTTCTATCTCTATGCGGAGACCAATATGCAGAGCATTCGGACCGAG
ATTGAGAAGATTAAGCCAGATTTCCTGATTATCGACTCTATCCAAACCATTATGAGTCCTGACATTTCTAGCGTACAAGG
TTCTGTCAGTCAGGTCCGTGAAGTGACCAATGAACTCATGCAAATTGCCAAGACCAACAACATCGCAACCTTTATCGTCG
GTCACATGACCAAGGAAGGAACCCTGGCTGGACCTCGGACCTTGGAGCATATGGTAGACACCGTTCTTTATTTTGAAGGC
GAGCGGCAGCATACCTTCCGTATCTTGCGGGCGGTCAAAAACCGCTTTGGCTCTACCAACGAAATCGGCATTTTTGAAAT
GCAGTCACAGGGTTTGGTCGAAGTCCTCAATCCTAGCGAGGTCTTTCTGGAAGAGCGTTTGGACGGGGCGACAGGCTCTG
CTATTGTCGTGACCATGGAGGGCACCCGCCCTATCCTTGCCGAAGTGCAGGCTCTGGTGACCCCGAGCATGTTTGGCAAT
GCCAAGCGGACCACGACAGGCTTGGACTTCAACCGTGCCAGCTTGATTATGGCGGTTTTGGAAAAACGGGCAGGCCTGCT
CCTCCAAAACCAAGATGCCTACCTTAAGTCAGCTGGCGGTGTCAAACTGGATGAGCCAGCTATTGACCTGGCGGTCGCAG
TTGCCCTTGCCTCCAGTTACAAAGATAAGCCAACCAACCCACAAGAGTGCTTTATAGGGGAGATTGGCCTGACAGGTGAA
ATCCGCCGTGTCAATCGGATTGAGCAACGGATTAACGAAGCCGCTAAATTGGGCTTTACCAAGGTCTATGCTCCTAAAAA
TTCCCTGACCGGTATCAAGGTGCCCAAGGAAATAACGGTTATCGGCGTGGCAACAATTGGCGAAGTTCTGCAAAAAGTGT
TTAACTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(64-220)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

91.15

99.341

0.905
  radA Streptococcus pneumoniae Rx1

90.929

99.341

0.903
  radA Streptococcus pneumoniae D39

90.929

99.341

0.903
  radA Streptococcus pneumoniae R6

90.929

99.341

0.903
  radA Streptococcus pneumoniae TIGR4

90.929

99.341

0.903
  radA Streptococcus mitis SK321

90.708

99.341

0.901
  radA Bacillus subtilis subsp. subtilis str. 168

62.472

99.56

0.622