Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   KCV09_RS09365 Genome accession   NZ_CP073241
Coordinates   2027468..2028226 (-) Length   252 a.a.
NCBI ID   WP_025095393.1    Uniprot ID   A0AB38Z0B0
Organism   Acinetobacter soli strain M3-1-68     
Function   assists with secretin assembly and localization (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2022468..2033226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCV09_RS09345 - 2023532..2024233 (-) 702 WP_004932928.1 tetratricopeptide repeat protein -
  KCV09_RS09350 hisS 2024265..2025557 (-) 1293 WP_151794860.1 histidine--tRNA ligase -
  KCV09_RS09355 ispG 2025554..2026669 (-) 1116 WP_004932923.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  KCV09_RS09360 - 2026695..2027477 (-) 783 WP_004943670.1 DUF4115 domain-containing protein -
  KCV09_RS09365 pilF 2027468..2028226 (-) 759 WP_025095393.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  KCV09_RS09370 rlmN 2028286..2029530 (-) 1245 WP_004932914.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  KCV09_RS09375 ndk 2029652..2030083 (-) 432 WP_004932912.1 nucleoside-diphosphate kinase -
  KCV09_RS09380 iscX 2030192..2030392 (-) 201 WP_004932909.1 Fe-S cluster assembly protein IscX -
  KCV09_RS09385 - 2030464..2031015 (-) 552 WP_004943680.1 phosphatase PAP2 family protein -
  KCV09_RS09390 - 2031041..2032324 (-) 1284 WP_151815169.1 glycosyltransferase family 1 protein -

Sequence

Protein


Download         Length: 252 a.a.        Molecular weight: 28565.55 Da        Isoelectric Point: 9.4949

>NTDB_id=558392 KCV09_RS09365 WP_025095393.1 2027468..2028226(-) (pilF) [Acinetobacter soli strain M3-1-68]
MLAALLLSGCQTTSIKKDPQKGAKVRTQLAAEYIKTGDLDAAKRALDHALKVEPKDSNANMMMGVLLQQEGSRPNLEKAD
EYFARAVALDPENAQARNNYGAYLYQMKRYHDAIQQLTRAGSTLGYDQRYLALENLGRIYLHLGGVSNAEKMFKQALQAN
RDSTVSMLELAEIYYLQHKIPASTEMYEQYVRTVGQKNQSARALWVGIRVARANADRTGVQVLVNQLRIFYPDSPEYQRY
MQLQYSTEAVWK

Nucleotide


Download         Length: 759 bp        

>NTDB_id=558392 KCV09_RS09365 WP_025095393.1 2027468..2028226(-) (pilF) [Acinetobacter soli strain M3-1-68]
ATGCTGGCGGCGCTTCTTTTATCTGGATGCCAAACGACTTCAATAAAAAAAGATCCGCAAAAAGGCGCGAAAGTCCGGAC
TCAACTTGCAGCAGAATATATCAAAACAGGTGATCTTGATGCTGCCAAGCGTGCGCTAGATCATGCACTCAAAGTCGAAC
CGAAAGACAGCAATGCCAACATGATGATGGGGGTGTTGCTACAGCAAGAGGGCAGTCGCCCAAATCTTGAAAAAGCCGAT
GAATATTTTGCACGTGCTGTAGCGCTGGATCCGGAGAATGCTCAGGCCAGAAACAACTATGGCGCTTATTTGTATCAGAT
GAAACGTTATCATGATGCAATTCAACAATTGACACGCGCCGGTAGTACATTAGGATATGACCAGCGCTATCTGGCATTGG
AAAATTTAGGTCGGATTTATTTGCATCTGGGGGGTGTTTCGAATGCTGAAAAAATGTTCAAACAGGCACTTCAGGCAAAC
CGTGATTCAACTGTTTCAATGTTAGAGTTAGCAGAAATTTATTATTTGCAACACAAAATTCCTGCCTCGACAGAGATGTA
TGAACAATATGTTCGAACTGTTGGGCAGAAAAATCAGAGTGCTCGTGCACTTTGGGTGGGAATTCGCGTCGCGAGAGCGA
ATGCCGACCGGACTGGGGTGCAAGTATTGGTAAATCAGCTTCGTATTTTTTACCCAGATAGTCCAGAATATCAACGTTAT
ATGCAATTACAGTACAGTACTGAGGCCGTATGGAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_10)

(132-161)

Tetratricopeptide repeat (TPR_8)

(23-56)

Tetratricopeptide repeat (TPR_16)

(76-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baylyi ADP1

85.317

100

0.853
  pilF Acinetobacter baumannii D1279779

74.8

99.206

0.742