Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GHH_RS00530 Genome accession   NC_020210
Coordinates   101496..102869 (+) Length   457 a.a.
NCBI ID   WP_015373719.1    Uniprot ID   L7ZVB7
Organism   Geobacillus sp. GHH01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 96496..107869
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GHH_RS00510 (GHH_c00990) ctsR 96867..97328 (+) 462 WP_011229590.1 transcriptional regulator CtsR -
  GHH_RS00515 (GHH_c01000) - 97344..97892 (+) 549 WP_011229591.1 UvrB/UvrC motif-containing protein -
  GHH_RS00520 (GHH_c01010) - 97898..98989 (+) 1092 WP_011229592.1 protein arginine kinase -
  GHH_RS00525 (GHH_c01020) clpC 98986..101418 (+) 2433 WP_011229593.1 ATP-dependent protease ATP-binding subunit ClpC -
  GHH_RS00530 (GHH_c01030) radA 101496..102869 (+) 1374 WP_015373719.1 DNA repair protein RadA Machinery gene
  GHH_RS00535 (GHH_c01040) - 103178..104272 (+) 1095 WP_012820471.1 PIN/TRAM domain-containing protein -
  GHH_RS00540 (GHH_c01050) ispD 104294..104980 (+) 687 WP_015373720.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GHH_RS00545 (GHH_c01060) ispF 104995..105471 (+) 477 WP_013143976.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  GHH_RS00550 (GHH_c01070) gltX 105673..107145 (+) 1473 WP_011229598.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49525.19 Da        Isoelectric Point: 7.8885

>NTDB_id=55815 GHH_RS00530 WP_015373719.1 101496..102869(+) (radA) [Geobacillus sp. GHH01]
MAKKKTKFVCQECGYESAKWLGRCPGCQTWNSFVEEMEQEKPTVRGAFLHSERAGSAKPVPITAVEAVQEPRMKTNSAEL
DRVLGGGIVKGSLVLIGGDPGIGKSTLLLQTSAQLAAAGHQVLYVSGEESVKQVKLRAGRLRAECDRLYVLAEADLEYIV
AAVETVQPACVIIDSIQTVYRTDISSAPGSVAQVRECTAELMKLAKTKGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYF
EGERHHTYRILRAVKNRFGSTNEIGIFEMRDIGLKEVENPSEVFLEERSRGAAGSTVVAAMEGTRPVLVEIQALVSPTSF
GNPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDRPTNPADVIIGEVGLT
GEVRRVSRIEQRVQEAVKLGFSRIIVPKNNLAGWQPPKGVQVIGVSHVAEALEHTMC

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=55815 GHH_RS00530 WP_015373719.1 101496..102869(+) (radA) [Geobacillus sp. GHH01]
ATGGCGAAGAAGAAAACGAAGTTCGTTTGCCAAGAGTGTGGATATGAGTCGGCCAAATGGCTTGGACGCTGTCCGGGATG
CCAAACATGGAATTCGTTTGTTGAGGAAATGGAGCAGGAGAAACCGACTGTGCGCGGCGCATTTCTTCATTCGGAGCGGG
CGGGTTCGGCCAAACCGGTCCCGATTACAGCCGTGGAGGCGGTGCAAGAGCCGCGCATGAAAACGAACAGCGCGGAGCTC
GATCGCGTGTTGGGCGGTGGAATCGTCAAAGGTTCGCTCGTTTTGATCGGCGGCGACCCTGGCATTGGCAAATCAACGTT
GCTTTTGCAAACATCGGCGCAGCTGGCCGCCGCTGGGCATCAAGTGCTGTATGTCTCCGGTGAGGAATCGGTCAAACAAG
TAAAGCTGCGCGCTGGACGCCTTCGTGCGGAGTGCGATCGGCTGTACGTGTTGGCGGAAGCAGATTTGGAATACATTGTG
GCAGCGGTTGAAACGGTTCAACCTGCTTGTGTCATCATTGATTCGATTCAGACGGTGTATCGCACCGATATTTCCTCTGC
ACCGGGCAGCGTCGCCCAAGTGCGCGAATGCACGGCCGAGCTGATGAAGCTGGCGAAAACAAAAGGCATTGCCATTTTTA
TCGTCGGTCATGTGACGAAGGAAGGGGCGATCGCCGGGCCGCGCCTGCTCGAACATATGGTGGACACCGTTCTCTATTTT
GAAGGGGAGCGACACCATACGTATCGCATTTTGCGAGCGGTGAAAAACCGTTTTGGCTCAACGAACGAAATCGGCATTTT
TGAGATGCGCGACATTGGGCTGAAGGAAGTGGAAAACCCATCAGAAGTGTTTTTAGAAGAACGGTCGCGCGGAGCGGCTG
GCTCGACGGTCGTGGCGGCGATGGAAGGGACGCGGCCGGTGCTCGTGGAAATCCAGGCGCTCGTCTCGCCGACGAGCTTC
GGCAACCCGAGACGGATGGCAACCGGCCTTGACCATAACCGTGTGTCGCTTTTGATGGCCGTGCTCGAAAAGCGGGTTGG
GCTGCTGTTGCAAAACCAAGATGCTTATTTGAAAGTGGCGGGCGGCGTGAAGCTTGATGAGCCGGCAATTGACTTGGCGG
TCGCTGTCAGCATCGCCTCGAGCTTTCGCGATCGGCCGACGAATCCGGCGGATGTCATTATCGGCGAAGTCGGCTTAACT
GGAGAGGTGCGCCGCGTCTCGCGCATCGAACAGCGCGTGCAGGAGGCGGTAAAATTAGGCTTTTCCCGCATCATTGTGCC
AAAAAACAATTTAGCTGGCTGGCAGCCGCCAAAAGGTGTGCAAGTGATCGGTGTTTCGCATGTGGCCGAGGCGCTTGAGC
ATACAATGTGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB L7ZVB7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.179

100

0.742
  radA Streptococcus mitis NCTC 12261

65.784

99.125

0.652
  radA Streptococcus mitis SK321

65.784

99.125

0.652
  radA Streptococcus pneumoniae Rx1

65.784

99.125

0.652
  radA Streptococcus pneumoniae D39

65.784

99.125

0.652
  radA Streptococcus pneumoniae R6

65.784

99.125

0.652
  radA Streptococcus pneumoniae TIGR4

65.784

99.125

0.652

Multiple sequence alignment