Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   H045_RS22945 Genome accession   NC_020209
Coordinates   5227155..5228480 (+) Length   441 a.a.
NCBI ID   WP_015373581.1    Uniprot ID   A0AAP2RXV4
Organism   Pseudomonas poae RE*1-1-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5222155..5233480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H045_RS22925 (H045_22910) - 5222231..5224696 (-) 2466 WP_015373578.1 penicillin-binding protein 1A -
  H045_RS22930 (H045_22915) pilM 5224913..5225569 (+) 657 WP_015373579.1 type IV pilus biogenesis protein PilM -
  H045_RS22935 (H045_22920) - 5225566..5226117 (+) 552 WP_003237320.1 PilN domain-containing protein -
  H045_RS22940 (H045_22925) - 5226114..5227085 (+) 972 WP_015373580.1 pilus assembly protein PilP -
  H045_RS22945 (H045_22930) pilQ 5227155..5228480 (+) 1326 WP_015373581.1 type IV pilus secretin PilQ Machinery gene
  H045_RS22950 (H045_22935) aroK 5228485..5229003 (+) 519 WP_003237314.1 shikimate kinase AroK -
  H045_RS22955 (H045_22940) aroB 5229118..5230218 (+) 1101 WP_003237313.1 3-dehydroquinate synthase -
  H045_RS22960 (H045_22945) - 5230229..5231797 (+) 1569 WP_015373582.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47582.36 Da        Isoelectric Point: 4.8113

>NTDB_id=55810 H045_RS22945 WP_015373581.1 5227155..5228480(+) (pilQ) [Pseudomonas poae RE*1-1-14]
MAAAVPNRVDLIQLPPPGGAGAGAYTGDTLDLNFQNIDLRAALQQIADVAGLNLVASDEVQGSVTLRLKNVPWDQALDLV
LQSKGLDKRVKAGVLLVAPADELAARELLTLEARRQMADLAPLRRELLQVNYAKAADLAKLFQSVNGLEGTIDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGRLSRGNWGAGGLSKSLAEGAKPPE
NPTASPFVDLGSLAGASGLGVAFITDNFLLDLELSAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSSGATSV
SFKEASLSLEVTPQITPDDRVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVRDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=55810 H045_RS22945 WP_015373581.1 5227155..5228480(+) (pilQ) [Pseudomonas poae RE*1-1-14]
ATGGCTGCCGCTGTGCCCAATCGTGTGGACCTGATCCAGTTGCCGCCACCCGGCGGCGCAGGGGCGGGCGCCTATACCGG
CGACACACTGGACCTCAATTTCCAGAACATCGACCTGCGCGCCGCGTTGCAGCAGATCGCCGACGTGGCAGGCCTCAATC
TGGTTGCCAGTGACGAGGTGCAAGGCTCGGTCACCCTGCGCCTGAAGAATGTGCCCTGGGACCAGGCGCTGGACCTCGTG
CTGCAAAGCAAGGGCCTGGACAAGCGGGTGAAAGCCGGCGTGCTGCTGGTGGCGCCCGCTGACGAGCTGGCCGCGCGCGA
ACTGCTGACCCTGGAGGCGCGGCGGCAGATGGCCGACCTGGCGCCGTTGCGCCGCGAACTGTTGCAGGTCAATTACGCCA
AGGCGGCCGACCTGGCCAAGCTGTTCCAGTCGGTGAACGGCCTCGAGGGCACCATCGATGAACGCGGTTCGGTGGCCGTT
GATGACCGCACCAACAACATCATTGCCTACCAGACCGGTGAACGGCTCGAAGAGCTGCGGCGCATCGTGGCGCAGCTGGA
CATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAATGTCGACTATGACAAAAGCCTGGGCGTACGTT
GGGGTGGCCGGCTCAGCCGCGGTAACTGGGGCGCGGGAGGTCTCTCGAAATCCCTGGCCGAAGGCGCGAAGCCACCGGAA
AACCCGACCGCCTCACCCTTCGTCGACCTCGGCTCGCTCGCCGGCGCGTCGGGGCTGGGCGTCGCCTTTATCACCGATAA
TTTCCTGCTGGACCTGGAACTGAGCGCCATGGAAAAAACCGGCAATGGCGAAATCGTCTCCCAGCCCAAGGTGGTCACGT
CCGACAAGGAGACCGCGCGCATCCTCAAGGGCACCGAAATTCCTTATCAGGAGTCCAGCTCCAGCGGCGCCACCTCGGTG
TCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTCACTCCACAGATCACCCCCGACGACCGCGTGATCATGGAGGTGAAAGT
CACCAAGGATGAGCCCGATTATTTGAACAAACTCAACGACGTGCCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGC
TGGTCCGGGACGGCGAGACCATCGTCATCGGTGGGGTTTTCTCCAATACCCAGAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTGTTCTT
GACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(53-99)

Bacterial type II and III secretion system protein (Secretin)

(276-432)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.396

99.093

0.707
  comQ Acinetobacter baylyi ADP1

43.207

100

0.44
  pilQ Vibrio campbellii strain DS40M4

45.07

96.599

0.435
  pilQ Acinetobacter baumannii strain A118

41.164

100

0.433
  pilQ Acinetobacter baumannii D1279779

41.164

100

0.433
  pilQ Legionella pneumophila strain Lp02

43.839

95.692

0.42
  pilQ Legionella pneumophila strain ERS1305867

43.735

95.918

0.419
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.78

94.785

0.415
  pilQ Vibrio cholerae strain A1552

43.78

94.785

0.415

Multiple sequence alignment