Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L1O95_RS21375 Genome accession   NZ_CP091020
Coordinates   4223691..4224131 (+) Length   146 a.a.
NCBI ID   WP_000360895.1    Uniprot ID   B7LFX5
Organism   Escherichia coli strain STEC308     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4218691..4229131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L1O95_RS21355 aroP 4219689..4221062 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  L1O95_RS21360 ampE 4221105..4221959 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  L1O95_RS21365 ampD 4221956..4222507 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  L1O95_RS21370 nadC 4222595..4223488 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  L1O95_RS21375 pilA 4223691..4224131 (+) 441 WP_000360895.1 prepilin peptidase-dependent pilin Machinery gene
  L1O95_RS21380 pilB 4224141..4225526 (+) 1386 WP_033812852.1 type II secretion system protein GspE Machinery gene
  L1O95_RS21385 hofC 4225516..4226718 (+) 1203 WP_000157227.1 protein transport protein HofC -
  L1O95_RS21390 guaC 4226753..4227796 (-) 1044 WP_001217342.1 GMP reductase -
  L1O95_RS21395 - 4227952..4227996 (-) 45 WP_120795372.1 protein YacM -
  L1O95_RS21400 coaE 4228021..4228641 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15602.71 Da        Isoelectric Point: 4.3927

>NTDB_id=557489 L1O95_RS21375 WP_000360895.1 4223691..4224131(+) (pilA) [Escherichia coli strain STEC308]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTH
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=557489 L1O95_RS21375 WP_000360895.1 4223691..4224131(+) (pilA) [Escherichia coli strain STEC308]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACTGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCAC
TATGTTTCAGCCATGAGTGTGGCAAAAGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGACTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LFX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363