Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   KBW87_RS07215 Genome accession   NZ_CP072983
Coordinates   1517149..1518948 (+) Length   599 a.a.
NCBI ID   WP_057808833.1    Uniprot ID   -
Organism   Lactobacillus intestinalis strain DSM 6629     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1489420..1519422 1517149..1518948 within 0


Gene organization within MGE regions


Location: 1489420..1519422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBW87_RS07085 (KBW87_07105) - 1489420..1490661 (-) 1242 WP_255807069.1 RNA-guided endonuclease TnpB family protein -
  KBW87_RS07090 (KBW87_07110) - 1490967..1492022 (+) 1056 WP_083478866.1 CAP domain-containing protein -
  KBW87_RS07095 (KBW87_07115) - 1492150..1493377 (+) 1228 Protein_1346 lactate oxidase -
  KBW87_RS07100 (KBW87_07120) - 1493430..1494434 (-) 1005 WP_057810271.1 C40 family peptidase -
  KBW87_RS07105 (KBW87_07125) - 1494684..1495541 (-) 858 WP_255807070.1 C40 family peptidase -
  KBW87_RS07110 (KBW87_07130) - 1495741..1496301 (-) 561 WP_004040420.1 C40 family peptidase -
  KBW87_RS07115 (KBW87_07135) - 1496506..1497057 (-) 552 WP_057808796.1 guanylate kinase -
  KBW87_RS07120 (KBW87_07140) - 1497148..1497450 (+) 303 WP_057808798.1 DUF2187 domain-containing protein -
  KBW87_RS07125 (KBW87_07145) - 1497549..1499075 (+) 1527 WP_057808800.1 O-antigen ligase family protein -
  KBW87_RS07130 (KBW87_07150) - 1499110..1499736 (-) 627 WP_057808802.1 LVIS_2131 family protein -
  KBW87_RS07135 (KBW87_07155) nrdI 1499853..1500296 (+) 444 WP_004040410.1 class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI -
  KBW87_RS07140 (KBW87_07160) - 1500299..1501231 (+) 933 WP_057808804.1 ribonucleotide-diphosphate reductase subunit beta -
  KBW87_RS07145 (KBW87_07165) - 1501367..1501876 (-) 510 WP_057808806.1 hypothetical protein -
  KBW87_RS07150 (KBW87_07170) - 1502019..1502723 (+) 705 WP_157055106.1 C39 family peptidase -
  KBW87_RS07155 (KBW87_07175) - 1502917..1503387 (-) 471 WP_057808810.1 threonine/serine exporter family protein -
  KBW87_RS07160 (KBW87_07180) - 1503390..1504163 (-) 774 WP_057808812.1 threonine/serine exporter family protein -
  KBW87_RS07165 (KBW87_07185) - 1504180..1505721 (-) 1542 WP_057808814.1 ABC-F family ATP-binding cassette domain-containing protein -
  KBW87_RS07170 (KBW87_07190) - 1505769..1506128 (-) 360 WP_004040385.1 hypothetical protein -
  KBW87_RS07175 (KBW87_07195) - 1506245..1507465 (-) 1221 WP_057808816.1 NAD(P)/FAD-dependent oxidoreductase -
  KBW87_RS07180 (KBW87_07200) - 1507487..1508386 (-) 900 WP_057808818.1 prenyltransferase -
  KBW87_RS07185 (KBW87_07205) - 1508452..1509444 (+) 993 WP_057808820.1 polyprenyl synthetase family protein -
  KBW87_RS07190 (KBW87_07210) cydC 1509441..1511189 (-) 1749 WP_057808822.1 thiol reductant ABC exporter subunit CydC -
  KBW87_RS07195 (KBW87_07215) cydD 1511179..1512897 (-) 1719 WP_057808824.1 thiol reductant ABC exporter subunit CydD -
  KBW87_RS07200 (KBW87_07220) cydB 1512897..1513913 (-) 1017 WP_057808827.1 cytochrome d ubiquinol oxidase subunit II -
  KBW87_RS07205 (KBW87_07225) - 1513914..1515341 (-) 1428 WP_057808829.1 cytochrome ubiquinol oxidase subunit I -
  KBW87_RS07210 (KBW87_07230) - 1515715..1516992 (-) 1278 WP_057808831.1 LCP family protein -
  KBW87_RS07215 (KBW87_07235) pepF 1517149..1518948 (+) 1800 WP_057808833.1 oligoendopeptidase F Regulator
  KBW87_RS07220 (KBW87_07240) - 1519042..1519422 (+) 381 WP_057808835.1 hypothetical protein -

Sequence

Protein


Download         Length: 599 a.a.        Molecular weight: 68989.03 Da        Isoelectric Point: 4.7239

>NTDB_id=557140 KBW87_RS07215 WP_057808833.1 1517149..1518948(+) (pepF) [Lactobacillus intestinalis strain DSM 6629]
MALPTRNEVPEELTWDLSRIFKSDKDWENAYQNVKNDINDLADLKNTITNSGKDLYEAITQMLAVKRRFENIYVYATMAS
DVDTSNNHYLGYVARVQSLASQFEAVTSFLNPAILSIPEEKLSDFMKEEPRLKDYAHLIDTITKMRPHTLPAQEEKLIAD
ASDAMNTSESTFNVLTNSDMQYGYVQDEDGQMVQLSDGLYSNLIQSQNRDVRKMAFDVMYATYGQFENTLASTLSGVVKM
HNYNARVHNFKSARNASLSENGVNTNVYDTLLKEVNKHLDLLHRYVSLRKKILKLDDLQMWDMYVPLTGKPALSYNFEEA
KKEAKKALAPLGEDYLKHVDYIFNNRVIDPVESQNKVTGAYSGGAYDTDPYELLNWENNIDSLYTLVHETGHSMHSWYTR
HNQPYVYGDYPIFVAEIASTTNENILTEYFLDHIKDPKTRAFILNYYLDSFKGTLYRQTQFAEFEQFIHEADANGEPLTA
EVLDNFYGELNQRYYGDSVTPGGDIAKEWSRIPHFYYNFYVYQYATGFAAATALANKVVHGDQKDKEAYIDYLKSGSSDY
PVEIMKRAGVDMTKPDYLEDAFKTFEKRLNEFESLIDQF

Nucleotide


Download         Length: 1800 bp        

>NTDB_id=557140 KBW87_RS07215 WP_057808833.1 1517149..1518948(+) (pepF) [Lactobacillus intestinalis strain DSM 6629]
ATGGCTTTACCAACAAGAAACGAAGTCCCAGAAGAATTAACTTGGGATTTGAGTCGCATTTTCAAATCAGATAAAGATTG
GGAAAATGCGTACCAAAACGTCAAAAACGATATCAATGATTTAGCAGATTTAAAAAATACTATTACCAATTCTGGAAAAG
ATCTGTACGAAGCTATCACTCAAATGTTGGCCGTAAAGCGGCGCTTTGAAAACATTTATGTCTATGCCACAATGGCAAGC
GATGTTGATACTTCAAATAATCATTATTTAGGCTATGTCGCCCGTGTCCAAAGCTTAGCTAGTCAATTTGAAGCAGTGAC
TTCCTTCCTTAATCCTGCGATCCTTTCTATCCCAGAAGAAAAGCTCTCTGACTTTATGAAAGAGGAACCACGTTTAAAGG
ATTATGCTCATTTAATTGACACCATCACCAAGATGCGTCCCCATACTCTTCCAGCCCAAGAAGAAAAATTAATTGCTGAC
GCGAGTGATGCAATGAATACTTCAGAAAGCACCTTTAACGTTTTAACTAACTCGGACATGCAATACGGATATGTTCAAGA
TGAAGATGGTCAAATGGTCCAACTTTCTGATGGGCTATATTCCAATTTAATTCAATCTCAAAACCGTGATGTACGTAAAA
TGGCCTTTGATGTGATGTATGCAACTTATGGTCAATTTGAAAACACACTAGCTTCAACTTTATCTGGCGTAGTAAAAATG
CATAACTATAACGCTCGGGTTCACAATTTTAAGTCTGCACGGAATGCTTCTCTTAGCGAAAATGGCGTAAATACCAATGT
TTATGACACATTGTTAAAAGAAGTTAACAAGCATCTTGATCTTCTCCACCGCTACGTTAGTTTACGTAAAAAGATTCTCA
AGCTTGACGATTTACAAATGTGGGATATGTATGTGCCTTTGACTGGAAAACCTGCTCTTTCATATAATTTTGAAGAAGCT
AAAAAAGAAGCTAAAAAAGCTTTAGCTCCTCTTGGAGAAGATTATTTAAAGCATGTCGATTATATTTTTAACAATCGAGT
AATTGACCCCGTAGAAAGCCAAAACAAGGTCACTGGCGCTTATTCTGGAGGAGCCTACGATACTGATCCATATGAACTTT
TAAACTGGGAAAACAATATTGACTCTCTTTATACTTTAGTTCATGAAACTGGCCACTCAATGCATTCATGGTACACCCGT
CACAATCAACCTTATGTTTATGGGGACTACCCGATTTTTGTTGCAGAAATTGCATCAACTACTAACGAAAATATTTTGAC
GGAATATTTCCTGGACCATATTAAGGATCCAAAAACCCGGGCCTTCATTTTAAATTATTATCTTGATTCATTTAAAGGTA
CTCTTTATCGTCAAACTCAATTTGCGGAATTCGAACAATTTATTCATGAAGCCGATGCCAATGGTGAACCCTTAACTGCT
GAAGTTTTAGATAATTTTTACGGTGAGCTCAACCAACGCTACTACGGCGACAGTGTTACACCAGGTGGAGATATTGCTAA
GGAATGGTCACGTATTCCTCACTTCTACTACAACTTCTATGTATATCAATATGCGACCGGCTTTGCAGCAGCGACAGCTT
TAGCAAATAAAGTGGTTCATGGCGATCAAAAAGATAAAGAAGCTTATATTGATTATCTTAAGTCTGGTTCAAGCGATTAT
CCTGTTGAAATCATGAAACGTGCAGGTGTTGATATGACTAAACCTGATTACTTAGAAGATGCTTTCAAGACCTTTGAAAA
ACGACTAAATGAATTTGAAAGTTTGATTGATCAATTCTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(114-181)

Peptidase family M3 (Peptidase_M3)

(203-583)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50.842

99.165

0.504