Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   KBW87_RS01725 Genome accession   NZ_CP072983
Coordinates   340742..342118 (+) Length   458 a.a.
NCBI ID   WP_057809284.1    Uniprot ID   -
Organism   Lactobacillus intestinalis strain DSM 6629     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 335742..347118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBW87_RS01700 (KBW87_01695) - 335745..337619 (+) 1875 Protein_327 peptide cleavage/export ABC transporter -
  KBW87_RS01705 (KBW87_01700) - 337630..338214 (+) 585 WP_057809293.1 hypothetical protein -
  KBW87_RS01710 (KBW87_01705) pepC 338359..339705 (-) 1347 WP_057809291.1 aminopeptidase C -
  KBW87_RS01715 (KBW87_01710) - 339759..340055 (-) 297 WP_057809289.1 hypothetical protein -
  KBW87_RS01720 (KBW87_01715) - 340191..340742 (+) 552 WP_057809287.1 dUTP diphosphatase -
  KBW87_RS01725 (KBW87_01720) radA 340742..342118 (+) 1377 WP_057809284.1 DNA repair protein RadA Machinery gene
  KBW87_RS01730 (KBW87_01725) gltX 342193..343692 (+) 1500 WP_004045519.1 glutamate--tRNA ligase -
  KBW87_RS01735 (KBW87_01730) cysS 343786..345219 (+) 1434 WP_057809282.1 cysteine--tRNA ligase -
  KBW87_RS01740 (KBW87_01735) - 345212..345655 (+) 444 WP_057809279.1 Mini-ribonuclease 3 -
  KBW87_RS01745 (KBW87_01740) rlmB 345642..346397 (+) 756 WP_004045516.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  KBW87_RS01750 (KBW87_01745) - 346554..347117 (+) 564 WP_057809277.1 sigma-70 family RNA polymerase sigma factor -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 50325.97 Da        Isoelectric Point: 7.0888

>NTDB_id=557129 KBW87_RS01725 WP_057809284.1 340742..342118(+) (radA) [Lactobacillus intestinalis strain DSM 6629]
MARVKTQYKCRSCGYISASYLGRCPNCGAWNQFEKETQEVQKRSAKQSPSQLIKKTGLSEPVKLENIKAEKEDRIKTKSE
ELNRVLGGGIVPGSLVLIGGDPGIGKSTLMLQIMSDIAEKYKVLYVSGEESANQIKLRADRLGVGKSDMLLYPETDMHDI
REQIDDLKPDFVVIDSIQTMNEPSLDSMTGSASQVREVTSELMKIAKMNAITVFVIGHVTKEGAIAGPKILEHMVDTVLY
FEGDEHHSYRILHSVKNRFGAANEIGMFEMVNEGLREVSNPSSIFLDQRLPQSTGSAIVVSLEGTRPLLAEIQALVTPTV
FGYAKRTTSGIDYNRAALLLAVLEKRGNLMLQNQDVYLTATGGIKLNEPAIDLAIAMSVASSYNNKEISPTDCFVGEVGL
TGEVRRVNKIESRITEAAKVGFKRIFIPRHNMDKSLTDLGIEVIPVSSIPQALKLVFG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=557129 KBW87_RS01725 WP_057809284.1 340742..342118(+) (radA) [Lactobacillus intestinalis strain DSM 6629]
ATGGCTCGAGTAAAAACTCAATATAAATGCCGCTCTTGTGGCTACATTTCGGCTAGCTATTTAGGTCGTTGTCCTAATTG
TGGTGCATGGAATCAATTTGAAAAAGAAACTCAAGAAGTTCAAAAACGCTCAGCTAAGCAATCACCTAGTCAATTGATTA
AAAAGACTGGATTGAGTGAGCCGGTTAAATTAGAAAATATCAAGGCAGAAAAAGAAGACCGAATTAAAACTAAGTCTGAA
GAATTAAACCGAGTTTTAGGAGGAGGAATTGTTCCCGGATCACTGGTTTTAATTGGGGGAGATCCTGGAATCGGTAAATC
CACTTTGATGCTGCAAATCATGAGCGATATCGCCGAAAAATATAAGGTTTTATATGTTTCTGGAGAAGAGTCTGCCAATC
AAATTAAGCTTCGCGCAGATCGTTTGGGCGTTGGTAAAAGCGACATGCTTTTATACCCGGAAACAGATATGCATGACATT
CGCGAGCAAATTGATGATTTAAAGCCTGACTTTGTTGTAATTGACTCGATTCAGACTATGAATGAACCAAGTTTAGATTC
CATGACTGGTTCAGCATCTCAAGTACGTGAAGTAACTAGTGAACTTATGAAAATTGCCAAGATGAATGCAATTACTGTTT
TTGTAATTGGCCATGTGACTAAGGAAGGGGCAATTGCTGGTCCAAAGATTTTGGAACATATGGTGGATACGGTGCTTTAT
TTTGAGGGAGATGAACACCATTCCTACCGAATTTTGCACTCAGTAAAGAATCGTTTTGGGGCTGCTAATGAAATTGGAAT
GTTTGAAATGGTCAATGAAGGATTAAGAGAGGTAAGCAATCCGTCTTCAATTTTTCTTGATCAAAGATTACCCCAATCCA
CTGGGTCGGCGATTGTAGTTTCACTTGAAGGAACGCGCCCACTATTAGCAGAAATTCAGGCTTTAGTGACGCCGACAGTT
TTTGGTTATGCTAAGCGAACTACTTCCGGAATTGATTATAATCGAGCAGCACTTCTTTTAGCGGTTTTAGAAAAACGAGG
CAACTTAATGCTTCAAAACCAGGATGTATATTTGACTGCAACTGGTGGGATTAAGTTGAATGAACCTGCAATTGACTTGG
CAATTGCAATGTCTGTCGCATCCAGCTATAACAACAAGGAAATTTCACCAACTGATTGTTTTGTCGGCGAAGTGGGCTTA
ACTGGGGAAGTACGACGCGTTAATAAAATTGAATCGCGAATTACTGAAGCTGCTAAAGTGGGGTTTAAAAGGATTTTTAT
TCCCCGACATAATATGGATAAAAGCTTAACTGATTTAGGAATTGAAGTGATTCCAGTTTCCAGCATTCCACAAGCTTTGA
AACTTGTTTTTGGTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-431)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-34)

AAA domain (AAA_25)

(66-223)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

58.206

99.782

0.581
  radA Streptococcus pneumoniae Rx1

58.206

99.782

0.581
  radA Streptococcus pneumoniae D39

58.206

99.782

0.581
  radA Streptococcus pneumoniae R6

58.206

99.782

0.581
  radA Streptococcus pneumoniae TIGR4

58.206

99.782

0.581
  radA Streptococcus mitis NCTC 12261

58.206

99.782

0.581
  radA Bacillus subtilis subsp. subtilis str. 168

55.628

100

0.561