Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   IBG17_RS20480 Genome accession   NZ_CP072961
Coordinates   4232025..4233458 (+) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter sp. MGH 14     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4227025..4238458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IBG17_RS20450 - 4227592..4228569 (+) 978 WP_022651914.1 calcium/sodium antiporter -
  IBG17_RS20455 kdsD 4228583..4229569 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  IBG17_RS20460 kdsC 4229584..4230150 (+) 567 WP_003861856.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  IBG17_RS20465 lptC 4230147..4230722 (+) 576 WP_003861858.1 LPS export ABC transporter periplasmic protein LptC -
  IBG17_RS20470 lptA 4230691..4231245 (+) 555 WP_003861860.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  IBG17_RS20475 lptB 4231252..4231977 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  IBG17_RS20480 rpoN/rpoN1 4232025..4233458 (+) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  IBG17_RS20485 hpf 4233481..4233768 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  IBG17_RS20490 ptsN 4233851..4234342 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  IBG17_RS20495 rapZ 4234388..4235242 (+) 855 WP_003861870.1 RNase adapter RapZ -
  IBG17_RS20500 npr 4235239..4235511 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  IBG17_RS20505 mtgA 4235551..4236276 (-) 726 WP_003861874.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  IBG17_RS20510 elbB 4236273..4236926 (-) 654 WP_003861876.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=557051 IBG17_RS20480 WP_003861864.1 4232025..4233458(+) (rpoN/rpoN1) [Enterobacter sp. MGH 14]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=557051 IBG17_RS20480 WP_003861864.1 4232025..4233458(+) (rpoN/rpoN1) [Enterobacter sp. MGH 14]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTCCAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAGACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCACTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACTACCCAATCGCTTCAGGACTACCTGATGTGGCAGGTGGAGCTGACGCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGTGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCTAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAACCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATTCAGACCAG
CGAACCGGAATATGTCATTCCCGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATTAAGAGTCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATAGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCCATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48