Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   KAQ61_RS15625 Genome accession   NZ_CP072916
Coordinates   3311431..3313011 (-) Length   526 a.a.
NCBI ID   WP_210929762.1    Uniprot ID   -
Organism   Comamonas aquatica strain NEB418     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3306431..3318011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KAQ61_RS15610 (KAQ61_15610) - 3307484..3307972 (-) 489 WP_210929760.1 putative toxin-antitoxin system toxin component, PIN family -
  KAQ61_RS15615 (KAQ61_15615) - 3307976..3309259 (-) 1284 WP_043383713.1 THUMP domain-containing class I SAM-dependent RNA methyltransferase -
  KAQ61_RS15620 (KAQ61_15620) - 3309412..3311238 (+) 1827 WP_210929761.1 methyl-accepting chemotaxis protein -
  KAQ61_RS15625 (KAQ61_15625) rpoN/rpoN1 3311431..3313011 (-) 1581 WP_210929762.1 RNA polymerase factor sigma-54 Machinery gene
  KAQ61_RS15630 (KAQ61_15630) lptB 3313062..3313832 (-) 771 WP_045265905.1 LPS export ABC transporter ATP-binding protein -
  KAQ61_RS15635 (KAQ61_15635) lptA 3313832..3314470 (-) 639 WP_210929763.1 lipopolysaccharide transport periplasmic protein LptA -
  KAQ61_RS15640 (KAQ61_15640) - 3314574..3315221 (-) 648 WP_042417634.1 thiol:disulfide interchange protein DsbA/DsbL -
  KAQ61_RS15645 (KAQ61_15645) - 3315277..3315927 (-) 651 WP_210929764.1 SPOR domain-containing protein -
  KAQ61_RS15650 (KAQ61_15650) argS 3315958..3317667 (-) 1710 WP_210929765.1 arginine--tRNA ligase -

Sequence

Protein


Download         Length: 526 a.a.        Molecular weight: 57872.28 Da        Isoelectric Point: 4.9005

>NTDB_id=556873 KAQ61_RS15625 WP_210929762.1 3311431..3313011(-) (rpoN/rpoN1) [Comamonas aquatica strain NEB418]
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELSQEVEQMLDDNPFLELASDDAPREEFGLQAADEPAQHEGGSVDETPY
SIADSAGAASAEGQKDQESAAPDSDVPSDWEGDGSVDIAHDDSEWGGDAPARGNRNDDEDAPTALDLAGAHESLTDFLHQ
QALSLRLSEIDRYALRFLIENLNDDGYLEDPLQALAEGLAGTDDLEQLDELVHRFTVALRLLQSLEPVGVGARDLAECLR
LQLLHLHREDPQDEMLGVALQVCAQPLEMLARRDVRKLMQACGLPEAKVRAALGVIARLEPKPGRRFTDVERHIIVPDVI
VKKTARKGGDGLAQFSVQLNPDVMPRLRVHDVYANALRSHKGSDGHAGLQQRLQEARWFIKNVQQRFDTILRVSKAIVDR
QKNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPHGTYELKFFFGSGLGTETGGNASSTAVRALIKQFVAA
ESPKKPLSDSQIADMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1581 bp        

>NTDB_id=556873 KAQ61_RS15625 WP_210929762.1 3311431..3313011(-) (rpoN/rpoN1) [Comamonas aquatica strain NEB418]
TTGAAGCCAGGTTTGTCACTGCGGGTCTCACAGCATCTGGCGCTCACGCCGCAGTTGCAGCAATCCATCCGGCTGTTGCA
GCTATCCACCCTGGAGTTGTCCCAGGAAGTCGAGCAAATGCTCGACGACAACCCGTTTCTGGAGCTGGCCTCGGACGACG
CCCCGCGCGAGGAGTTCGGCCTGCAGGCCGCGGACGAGCCCGCCCAGCACGAGGGCGGCTCGGTCGACGAAACACCATAT
TCCATAGCAGACAGCGCAGGTGCCGCAAGCGCTGAAGGCCAAAAAGACCAGGAATCCGCGGCGCCCGACAGCGACGTGCC
CAGCGACTGGGAGGGCGACGGCAGCGTCGACATCGCGCACGACGACAGCGAATGGGGCGGCGACGCCCCGGCGCGCGGCA
ACCGCAATGACGACGAGGATGCCCCCACGGCCCTGGACCTGGCGGGCGCGCACGAGTCGCTGACCGATTTCCTGCACCAG
CAGGCGCTGTCGCTGCGCCTGTCCGAGATCGACCGCTATGCGCTGCGTTTCCTGATCGAAAACCTCAACGACGATGGCTA
CCTCGAGGACCCGCTGCAGGCGTTGGCTGAGGGCCTGGCCGGCACCGACGATCTGGAGCAGCTGGACGAGCTGGTGCACC
GCTTCACCGTGGCCCTGCGCCTGCTGCAGTCGCTGGAGCCCGTGGGCGTGGGCGCCCGCGACCTGGCCGAATGCCTGCGC
CTGCAGCTGCTGCACCTGCACCGCGAGGATCCGCAGGACGAGATGCTGGGGGTGGCGCTGCAGGTCTGCGCCCAGCCGCT
GGAAATGCTGGCGCGGCGCGATGTGCGCAAGCTGATGCAGGCCTGCGGCCTGCCCGAGGCCAAGGTGCGCGCCGCGCTGG
GCGTCATCGCTCGGCTGGAGCCCAAGCCGGGGCGGCGCTTCACCGATGTCGAGCGCCACATCATCGTGCCCGACGTGATC
GTCAAGAAAACAGCGCGCAAGGGGGGGGATGGCCTGGCGCAGTTCAGCGTGCAGCTGAACCCGGACGTGATGCCGCGCCT
GCGCGTGCACGACGTGTACGCCAACGCCCTGCGCAGCCACAAGGGCAGCGACGGGCACGCCGGCCTGCAGCAGCGGCTGC
AGGAGGCGCGCTGGTTCATCAAGAACGTGCAGCAGCGCTTCGACACCATCCTGCGCGTGTCCAAGGCCATCGTCGACCGG
CAGAAGAACTTTTTCGTGCACGGCGAGCTGGCCATGCGCCCGCTGGTGCTGCGCGACATTGCCGACGAACTGGGCCTGCA
CGAATCGACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCGCACGGCACCTACGAGCTGAAGTTCTTCTTCG
GCTCGGGCCTGGGCACGGAGACCGGGGGCAACGCCTCCAGCACGGCGGTGCGCGCGCTGATCAAGCAGTTCGTCGCGGCC
GAGAGCCCGAAGAAACCGCTGTCCGACAGCCAGATCGCGGACATGCTCAAGGAGCAGGGCATCGAGTGCGCGCGCCGCAC
CGTGGCCAAGTACCGCGAGGCCTTGAAGATCGCCCCGGCCAACCTGCGCAAGGCGCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(148-353)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-49)

Sigma-54, DNA binding domain (Sigma54_DBD)

(369-524)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.731

100

0.532