Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   J9537_RS00685 Genome accession   NZ_CP072888
Coordinates   135118..136491 (+) Length   457 a.a.
NCBI ID   WP_010747276.1    Uniprot ID   R2NRU4
Organism   Enterococcus raffinosus strain F162_2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 130118..141491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J9537_RS00665 (J9537_00665) - 130191..130937 (+) 747 WP_218256348.1 TIGR03915 family putative DNA repair protein -
  J9537_RS00670 (J9537_00670) - 131061..132017 (+) 957 WP_218256349.1 cation diffusion facilitator family transporter -
  J9537_RS00675 (J9537_00675) - 132591..134390 (+) 1800 WP_218256350.1 glycerophosphodiester phosphodiesterase -
  J9537_RS00680 (J9537_00680) - 134581..135087 (+) 507 WP_218256351.1 dUTP diphosphatase -
  J9537_RS00685 (J9537_00685) radA 135118..136491 (+) 1374 WP_010747276.1 DNA repair protein RadA Machinery gene
  J9537_RS00690 (J9537_00690) - 136569..137723 (+) 1155 WP_010747275.1 PIN/TRAM domain-containing protein -
  J9537_RS00695 (J9537_00695) gltX 137821..139278 (+) 1458 WP_218256352.1 glutamate--tRNA ligase -
  J9537_RS00700 (J9537_00700) epsC 139876..140409 (+) 534 WP_218256353.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 50036.52 Da        Isoelectric Point: 7.7029

>NTDB_id=556619 J9537_RS00685 WP_010747276.1 135118..136491(+) (radA) [Enterococcus raffinosus strain F162_2]
MAKKSKTQFICQECGYVSPKYLGRCPNCGNWNTMVEEIEQNHPEDRRNRVSLTGERATPTKLKDVVFKKETRVQTELAEL
NRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSHQLAATGGTVLYVSGEESAQQIKLRAERLGSSETDFYLYAETDMGEIS
RTIENLKPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGNIAGPRMLEHMVDTVLYF
EGEKHHSFRILRAVKNRFGSTNEIGIFEMRERGLVEVANPSQVFLEERLEGATGSAIVVAMEGSRPILVEVQALVTPTVF
GNAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAVAVAIASSYKENGTEATECFIGEIGLT
GEIRRVNSIEQRVKEASKLGFKKIYLPKNNMGDWKPPKGIELVPVATLGETLRKVFR

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=556619 J9537_RS00685 WP_010747276.1 135118..136491(+) (radA) [Enterococcus raffinosus strain F162_2]
TTGGCAAAAAAATCCAAGACACAATTTATCTGCCAAGAATGTGGTTATGTTTCGCCTAAGTATTTAGGGCGTTGTCCTAA
CTGCGGAAATTGGAACACGATGGTAGAGGAGATTGAGCAAAATCATCCCGAAGATCGACGCAACCGTGTCAGTTTAACCG
GAGAGCGTGCGACACCTACCAAATTAAAGGATGTCGTCTTCAAAAAGGAAACGCGCGTTCAGACAGAATTAGCTGAATTG
AATCGCGTTCTTGGCGGCGGAGTGGTTCCAGGTTCATTGGTATTAATCGGTGGGGACCCCGGTATTGGGAAATCCACCTT
ACTATTGCAAGTATCCCATCAATTAGCGGCAACCGGCGGTACAGTTTTGTATGTTTCTGGAGAAGAAAGTGCGCAGCAGA
TAAAATTGCGGGCGGAGCGCCTAGGATCAAGTGAGACAGATTTTTATTTATATGCGGAAACAGACATGGGAGAAATCAGC
CGAACGATTGAAAATCTGAAGCCGGATTATGTGATCATCGACTCGATCCAAACCATGACACAACCAGATATCACTAGTGT
GGCGGGCTCGGTCAGCCAGGTTCGGGAGACCACCGCAGAGCTGTTGAAGCTAGCGAAGACGAACGGTATTGCGATTTTTA
TCGTAGGTCATGTGACCAAAGAGGGAAATATCGCAGGTCCGCGAATGCTGGAGCATATGGTGGATACTGTTTTATACTTT
GAGGGCGAGAAGCATCACAGCTTTCGAATCTTACGCGCGGTAAAAAATCGTTTCGGTTCCACTAATGAAATCGGAATTTT
CGAGATGAGAGAACGAGGGCTGGTCGAAGTGGCCAATCCTTCACAGGTTTTCCTAGAGGAACGCCTAGAAGGTGCAACCG
GTTCAGCAATCGTGGTTGCCATGGAAGGCAGCCGACCAATTTTGGTTGAGGTGCAGGCATTGGTAACCCCCACTGTTTTC
GGCAACGCGAAGCGCACAACGACCGGCTTAGATTTCAACCGTGTTTCTTTGATCATGGCTGTTTTGGAAAAACGCGCCGG
GTTGCTGCTGCAAAATCAAGATGCCTACTTAAAAGCGGCAGGCGGGGTAAAATTAAATGAACCGGCCATTGATTTAGCGG
TGGCTGTGGCGATTGCCTCCAGCTACAAAGAAAACGGCACGGAAGCCACCGAATGCTTTATCGGTGAAATTGGTTTGACG
GGTGAAATTCGTCGGGTCAACAGCATTGAACAGCGGGTGAAAGAAGCCAGCAAATTGGGCTTCAAAAAAATCTATCTGCC
TAAGAACAATATGGGCGATTGGAAACCGCCAAAAGGAATCGAATTGGTTCCTGTGGCAACCTTAGGGGAAACCTTAAGAA
AAGTATTTCGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(57-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R2NRU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.123

99.781

0.74
  radA Streptococcus mitis SK321

73.904

99.781

0.737
  radA Streptococcus pneumoniae Rx1

73.904

99.781

0.737
  radA Streptococcus pneumoniae D39

73.904

99.781

0.737
  radA Streptococcus pneumoniae R6

73.904

99.781

0.737
  radA Streptococcus pneumoniae TIGR4

73.904

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

64.967

98.687

0.641