Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J8L76_RS07280 Genome accession   NZ_CP072875
Coordinates   1562977..1564692 (-) Length   571 a.a.
NCBI ID   WP_216131801.1    Uniprot ID   A0A975SQ70
Organism   Azospira inquinata strain Azo-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1557977..1569692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8L76_RS07255 (J8L76_07255) aspS 1558017..1559831 (-) 1815 WP_216131789.1 aspartate--tRNA ligase -
  J8L76_RS07260 (J8L76_07260) - 1559854..1560477 (-) 624 WP_216132211.1 DUF502 domain-containing protein -
  J8L76_RS07265 (J8L76_07265) - 1560477..1560725 (-) 249 WP_216131792.1 FmdB family zinc ribbon protein -
  J8L76_RS07270 (J8L76_07270) pilD 1560824..1561681 (-) 858 WP_216131795.1 prepilin peptidase Machinery gene
  J8L76_RS07275 (J8L76_07275) pilC 1561705..1562943 (-) 1239 WP_216131798.1 type II secretion system F family protein Machinery gene
  J8L76_RS07280 (J8L76_07280) pilB 1562977..1564692 (-) 1716 WP_216131801.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J8L76_RS07285 (J8L76_07285) - 1564872..1565714 (-) 843 WP_216131803.1 inner membrane protein YpjD -
  J8L76_RS07290 (J8L76_07290) ffh 1565745..1567100 (+) 1356 WP_216131806.1 signal recognition particle protein -
  J8L76_RS07295 (J8L76_07295) - 1567152..1567754 (-) 603 WP_216131809.1 transglycosylase SLT domain-containing protein -
  J8L76_RS07300 (J8L76_07300) - 1567742..1569454 (-) 1713 WP_216131812.1 proline--tRNA ligase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62232.34 Da        Isoelectric Point: 5.0690

>NTDB_id=556493 J8L76_RS07280 WP_216131801.1 1562977..1564692(-) (pilB) [Azospira inquinata strain Azo-2]
MAATLQVPLSGLARALVQAERIKESEADTFMAQASSTGGTFIGQLVATKRFSARDIALFAAETFGYPLLDLDAYDDSQIP
TDAIDRKLIATHRVIPLNKRGNRLAIAVADPTNLRALDEIRFQTGLAVDPIVVEETKLTPLVAKHSEAAEDSLKAFASED
LNLEFAEEESEKADEAVTLEVDDAPVVKFIQKILLDAINDGASDVHFEPYEKYYRIRFRIDGVLREIATPPLVIKEKIAS
RIKVISRLDISEKRVPQDGRMKLVLSKNRAIDFRVSTLPTLHGEKIVMRILDPTSATLGIEALGYEPEQQAALMDAIHRP
YGMVLVTGPTGSGKTVSLYTCLNLLNDPGTNISTAEDPAEINLPGINQVNVNEKAGLTFSSALKAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPTTLERLKNMGVPTFNIAASVILITAQRLARRLCTCKVPLDVPRDTLLDAGFK
ESDLDGSWTLFGPRDGGCDRCKGTGYKGRVGIYQVMPITEEIQRIIMGNGNSMDIAAQAQKEGVKNLRQSGLLKVKQGVT
SLDEVLSTTNA

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=556493 J8L76_RS07280 WP_216131801.1 1562977..1564692(-) (pilB) [Azospira inquinata strain Azo-2]
ATGGCAGCAACGCTACAGGTACCCCTCAGCGGTTTAGCGCGTGCGCTGGTACAAGCGGAACGGATAAAGGAATCCGAAGC
CGATACCTTCATGGCTCAAGCATCGAGCACCGGGGGTACATTCATTGGCCAGCTAGTAGCCACCAAACGCTTTTCCGCCC
GGGATATTGCGCTCTTTGCCGCAGAAACCTTTGGTTACCCCCTGCTGGACCTGGATGCCTACGACGATAGTCAAATCCCT
ACGGATGCCATTGACCGAAAGCTCATTGCAACCCACCGGGTCATCCCACTCAATAAGCGGGGCAACCGGCTCGCCATTGC
TGTGGCCGACCCCACCAATCTGCGTGCTTTAGATGAGATCCGGTTTCAGACCGGCTTAGCCGTCGACCCGATTGTTGTTG
AAGAAACAAAACTTACCCCCCTGGTCGCAAAACATTCCGAAGCCGCCGAAGACTCCTTGAAAGCCTTTGCTTCGGAAGAT
TTGAACCTTGAATTCGCAGAAGAGGAATCGGAAAAGGCGGACGAAGCCGTCACCTTAGAGGTGGACGATGCACCTGTCGT
CAAATTTATTCAGAAAATATTGCTGGATGCTATTAACGATGGAGCCTCTGACGTCCATTTCGAACCCTATGAAAAGTATT
ACCGCATCCGCTTTCGTATCGACGGCGTATTAAGGGAAATAGCCACGCCCCCCTTGGTCATCAAGGAAAAAATCGCATCC
CGGATTAAAGTCATTTCCCGTTTGGATATTTCCGAAAAACGTGTTCCCCAAGACGGCCGGATGAAATTGGTTTTATCCAA
AAATCGGGCCATTGATTTTCGCGTTAGCACCCTCCCCACCCTGCATGGGGAAAAAATTGTTATGCGGATTTTGGACCCCA
CATCCGCCACCTTGGGTATTGAAGCGTTGGGTTATGAACCCGAGCAGCAAGCCGCCCTAATGGACGCCATCCACCGCCCC
TACGGCATGGTGCTAGTTACCGGTCCTACCGGCTCAGGGAAAACGGTTTCCCTTTATACTTGCCTGAATCTGCTCAACGA
CCCAGGAACCAATATTTCCACAGCGGAAGATCCTGCTGAAATCAATTTACCGGGAATTAATCAGGTTAACGTAAACGAAA
AAGCTGGCCTCACCTTTTCCTCCGCCCTAAAAGCCTTTTTGCGTCAAGATCCGGACGTCATCATGGTGGGGGAAATTCGG
GATTTGGAAACGGCAGAAATCGCCATCAAGGCGGCCCAAACCGGGCACATGGTGCTTTCTACCCTGCACACCAACGACGC
ACCGACGACCCTGGAACGGCTAAAGAATATGGGGGTTCCTACCTTCAATATCGCCGCCTCGGTGATTCTCATAACTGCAC
AGCGCTTGGCCCGCAGGCTCTGCACTTGCAAGGTTCCCCTTGATGTTCCGAGAGATACCTTACTGGATGCGGGCTTCAAA
GAAAGCGATCTGGACGGAAGCTGGACTTTGTTTGGCCCTCGAGATGGCGGCTGTGACCGCTGCAAGGGAACTGGCTATAA
AGGCCGGGTTGGCATTTACCAGGTCATGCCGATTACCGAAGAAATTCAGCGCATTATCATGGGCAATGGGAATTCCATGG
ATATTGCGGCTCAAGCCCAAAAAGAAGGGGTAAAGAACCTTCGCCAATCCGGCTTGTTGAAAGTAAAGCAAGGAGTGACC
TCCTTGGACGAAGTTCTTTCCACGACCAACGCCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.667

99.825

0.566
  pilB Acinetobacter baylyi ADP1

55.752

98.949

0.552
  pilB Legionella pneumophila strain ERS1305867

53.86

99.825

0.538
  pilF Neisseria gonorrhoeae MS11

52.465

99.475

0.522
  pilB Vibrio cholerae strain A1552

47.695

98.774

0.471
  pilB Vibrio campbellii strain DS40M4

46.277

98.774

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

45.633

98.249

0.448
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.472

97.548

0.405
  pilF Thermus thermophilus HB27

39.755

100

0.398