Detailed information NTG0556282

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: J9265_RS11750
• Genome accession: NZ_CP072847
• Coordinates: 2581949..2582965 (-)
• Length: 338 a.a.
• NCBI ID: WP_000520444.1
• Uniprot ID: A0A0F0B833
• Organism: Vibrio cholerae strain A1552
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2576949..2587965

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J9265_RS11725 (J9265_11725)	aroK	2577832..2578356 (-)	525	WP_000818616.1	shikimate kinase AroK	-
J9265_RS11730 (J9265_11730)	pilQ	2578543..2580258 (-)	1716	WP_000788426.1	type IV pilus secretin PilQ	Machinery gene
J9265_RS11735 (J9265_11735)	pilP	2580284..2580802 (-)	519	WP_000792032.1	pilus assembly protein PilP	Machinery gene
J9265_RS11740 (J9265_11740)	pilO	2580792..2581382 (-)	591	WP_000157690.1	type 4a pilus biogenesis protein PilO	Machinery gene
J9265_RS11745 (J9265_11745)	pilN	2581375..2581959 (-)	585	WP_000902745.1	PilN domain-containing protein	Machinery gene
J9265_RS11750 (J9265_11750)	pilM	2581949..2582965 (-)	1017	WP_000520444.1	type IV pilus assembly protein PilM	Machinery gene
J9265_RS11755 (J9265_11755)	-	2583108..2585585 (+)	2478	WP_000668733.1	penicillin-binding protein 1A	-
J9265_RS11760 (J9265_11760)	oxyR	2585659..2586552 (-)	894	WP_001025909.1	DNA-binding transcriptional regulator OxyR	-
J9265_RS11765 (J9265_11765)	-	2586692..2587423 (+)	732	WP_000495223.1	glutathione peroxidase	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37707.44 Da
• Isoelectric Point: 7.3328

>NTDB_id=556282 J9265_RS11750 WP_000520444.1 2581949..2582965(-) (pilM) [Vibrio cholerae strain A1552]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide

• Length: 1017 bp

>NTDB_id=556282 J9265_RS11750 WP_000520444.1 2581949..2582965(-) (pilM) [Vibrio cholerae strain A1552]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTTTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCA
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
GTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-221)

• Type IV pilus assembly protein PilM; (PilM_2) (241-328)

3D structure

• AlphaFold DB: A0A0F0B833

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	100	100	1
pilM	Vibrio campbellii strain DS40M4	48.521	100	0.485