Detailed information NTG0555938

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: J9253_RS19375
• Genome accession: NZ_CP072801
• Coordinates: 3999932..4001110 (-)
• Length: 392 a.a.
• NCBI ID: WP_028488344.1
• Uniprot ID: -
• Organism: Thiothrix litoralis strain AS
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3994932..4006110

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J9253_RS19360 (J9253_19360)	yciA	3996959..3997354 (+)	396	WP_210222473.1	acyl-CoA thioester hydrolase YciA	-
J9253_RS19365 (J9253_19365)	-	3997358..3998095 (-)	738	WP_210222474.1	M48 family metallopeptidase	-
J9253_RS19370 (J9253_19370)	argS	3998194..3999930 (+)	1737	WP_210222475.1	arginine--tRNA ligase	-
J9253_RS19375 (J9253_19375)	pilU	3999932..4001110 (-)	1179	WP_028488344.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
J9253_RS19380 (J9253_19380)	-	4001166..4001849 (+)	684	WP_210222476.1	pseudouridine synthase	-
J9253_RS19385 (J9253_19385)	ilvA	4001936..4003450 (-)	1515	WP_210222477.1	threonine ammonia-lyase, biosynthetic	-
J9253_RS19390 (J9253_19390)	rpiA	4003544..4004200 (+)	657	WP_210222478.1	ribose-5-phosphate isomerase RpiA	-
J9253_RS19395 (J9253_19395)	-	4004232..4004819 (+)	588	WP_210222479.1	DUF1461 domain-containing protein	-
J9253_RS19400 (J9253_19400)	ald	4004785..4005771 (+)	987	WP_210222480.1	alanine dehydrogenase	-

Sequence

Protein

• Length: 392 a.a.
• Molecular weight: 43731.58 Da
• Isoelectric Point: 6.8299

>NTDB_id=555938 J9253_RS19375 WP_028488344.1 3999932..4001110(-) (pilU) [Thiothrix litoralis strain AS]
MKLKDYLRILAHYDGSDLYLTADLEPKAKFQGKLKALDKVVMTPEMLKDMAYELMNKEQQQQFEHKPEMNLAISEEGVGR
FRVNIFKQRHKIAMVIRNIKTDIPNADKLGLPQVLKDVIMEKRGLILFVGGTGSGKSTSLAALIDHRNASADGHIITIED
PVEYVHPHKKCIINQREVGVDTDSYEDALKNTLRQAPDVILIGEIRAQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRHQLLMDLSLNLKAFVSQRLIPTIDGKRVAAIEILLGTPMIRDLIMKGDVHAIKEIMEKSEEQGMQTFDSHL
YKLYLAGQISLAETLRNADSPSNLKLKINLSGNLAKPRPTAAAAAPEAKSTAASADFMANLSLEPKPEVELT

Nucleotide

• Length: 1179 bp

>NTDB_id=555938 J9253_RS19375 WP_028488344.1 3999932..4001110(-) (pilU) [Thiothrix litoralis strain AS]
ATGAAACTCAAAGACTATCTGCGTATCCTCGCCCATTACGACGGTTCCGACCTGTACCTCACCGCCGATCTCGAACCCAA
AGCCAAATTCCAAGGCAAACTCAAAGCCCTCGATAAAGTCGTCATGACCCCGGAAATGCTCAAGGACATGGCTTACGAGC
TAATGAACAAAGAACAGCAGCAGCAGTTTGAACACAAACCCGAAATGAACCTCGCCATCAGCGAAGAAGGCGTTGGCCGT
TTCCGCGTCAATATTTTCAAGCAGCGCCACAAAATCGCGATGGTCATCCGCAATATCAAAACCGACATTCCCAACGCCGA
CAAACTCGGCCTGCCGCAAGTGCTCAAAGACGTGATCATGGAAAAACGCGGGCTGATCCTGTTCGTCGGCGGCACAGGTT
CGGGCAAATCGACTTCACTCGCCGCACTGATCGACCACCGCAATGCTTCGGCTGACGGGCATATCATCACCATCGAAGAC
CCGGTGGAATACGTGCATCCGCACAAAAAATGTATCATCAACCAGCGCGAAGTCGGCGTCGACACCGACAGCTACGAAGA
TGCGCTGAAAAATACCCTGCGCCAAGCGCCCGATGTGATCCTGATCGGTGAAATCCGCGCCCAAGAAACCATGGAACACG
CCCTAGCCTTCGCCGAAACAGGCCACCTGTGCCTGTCCACCCTGCACGCTAACAACGCCAACCAAGCGCTCGACCGCATC
ATCAACTTCTTCCCCGAAGAACGCCGCCACCAATTGCTGATGGATTTATCCCTCAACCTCAAAGCCTTCGTCTCGCAACG
CCTGATCCCCACCATTGACGGCAAGCGCGTTGCCGCCATCGAAATCCTGCTCGGCACACCGATGATTCGCGACCTGATCA
TGAAAGGCGACGTGCACGCCATCAAAGAAATCATGGAAAAGTCCGAAGAACAGGGAATGCAAACCTTCGACAGTCACCTC
TACAAGCTGTATCTGGCAGGGCAAATCTCACTGGCAGAAACCTTGCGCAACGCCGATTCCCCCAGCAACCTCAAGCTCAA
GATCAACCTGTCGGGGAATCTGGCGAAACCACGTCCGACAGCAGCGGCAGCAGCACCTGAAGCCAAATCCACGGCCGCAA
GTGCTGATTTCATGGCAAATTTGTCGCTGGAACCAAAACCAGAGGTGGAACTGACGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (35-244)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.102	89.796	0.513
pilU	Acinetobacter baylyi ADP1	54.318	91.582	0.497
pilU	Vibrio cholerae strain A1552	49.28	88.52	0.436
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.91	96.173	0.403