Detailed information NTG0555522

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: J8Y18_RS25690
• Genome accession: NZ_CP072774
• Coordinates: 5011502..5012851 (-)
• Length: 449 a.a.
• NCBI ID: WP_000879430.1
• Uniprot ID: -
• Organism: Bacillus cereus strain BC33
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5006502..5017851

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J8Y18_RS25670 (J8Y18_25670)	secA	5006827..5009334 (-)	2508	WP_000579385.1	preprotein translocase subunit SecA	-
J8Y18_RS25675 (J8Y18_25675)	raiA	5009609..5010151 (-)	543	WP_000671185.1	ribosome-associated translation inhibitor RaiA	-
J8Y18_RS25680 (J8Y18_25680)	cspC	5010474..5010671 (-)	198	WP_001990088.1	cold shock protein CspC	-
J8Y18_RS25685 (J8Y18_25685)	-	5010798..5011502 (-)	705	WP_002183001.1	ComF family protein	-
J8Y18_RS25690 (J8Y18_25690)	comFA	5011502..5012851 (-)	1350	WP_000879430.1	ATP-dependent helicase ComFA	Machinery gene
J8Y18_RS25695 (J8Y18_25695)	-	5012978..5014423 (-)	1446	WP_000499477.1	C40 family peptidase	-
J8Y18_RS25700 (J8Y18_25700)	-	5014584..5014898 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
J8Y18_RS25705 (J8Y18_25705)	-	5015071..5015913 (-)	843	WP_000684725.1	DegV family protein	-
J8Y18_RS25710 (J8Y18_25710)	-	5016150..5016785 (+)	636	WP_000926669.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51062.89 Da
• Isoelectric Point: 9.8494

>NTDB_id=555522 J8Y18_RS25690 WP_000879430.1 5011502..5012851(-) (comFA) [Bacillus cereus strain BC33]
MLAGKQLLLEELSSDLQRELNDLKKKGEIVCVQGVKKKNSKYMCQRCGNVDRWLFASFLCKRCSKVCTYCRKCIAMGRVS
ECAVLVRGIAERKREKNSNLLQWNGTLSTGQNLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVIVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKLKQGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKSLNHERIPRVLLQWLKMYLNKKYPVFL
FVPHIRYIEEISSLLKSLHNKVEGVHAEDPMRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=555522 J8Y18_RS25690 WP_000879430.1 5011502..5012851(-) (comFA) [Bacillus cereus strain BC33]
ATGCTAGCGGGGAAACAGTTGCTATTAGAAGAACTTTCTTCAGATTTACAGAGGGAATTAAACGATTTGAAAAAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGTGGAAACGTAGATCGGTGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCACATATTGCCGGAAGTGTATAGCGATGGGGAGAGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTCAAATTTGTTACAGTGGAACGGGACGTT
GTCTACTGGCCAGAATTTGGCGGCGCAAGGAGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGTATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCAAGAACGGATGTTGTTTTGGAATTAGCACCGAGATTACAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTAGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACCACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATAGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAAACAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCACCGTCATCCGTTGCCAGTTCCTCTATTTTGCTGGTGCGGAAATTGGAAGAAAA
GCCTCAACCATGAAAGAATTCCTCGAGTTTTACTACAATGGTTAAAGATGTACTTAAACAAAAAGTATCCTGTTTTTTTA
TTCGTTCCCCATATACGATATATAGAAGAAATAAGCTCGTTATTGAAATCATTGCATAATAAAGTTGAAGGGGTACATGC
AGAAGATCCGATGAGAAAAGAGAAAGTCGCAGCGTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTTGCAGTTTTAGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAGGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (107-254)

• Helicase conserved C-terminal domain (Helicase_C) (313-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.131	98.441	0.503