Detailed information NTG0555445

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: J8Y17_RS05760
• Genome accession: NZ_CP072769
• Coordinates: 1110425..1114150 (+)
• Length: 1241 a.a.
• NCBI ID: WP_193108998.1
• Uniprot ID: -
• Organism: Bacillus cereus strain BC07
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1105425..1119150

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J8Y17_RS05745 (J8Y17_05745)	-	1105583..1106176 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
J8Y17_RS05750 (J8Y17_05750)	lepB	1106233..1106796 (+)	564	WP_071729527.1	signal peptidase I	-
J8Y17_RS05755 (J8Y17_05755)	addB	1106913..1110428 (+)	3516	WP_211479888.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
J8Y17_RS05760 (J8Y17_05760)	addA	1110425..1114150 (+)	3726	WP_193108998.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
J8Y17_RS05765 (J8Y17_05765)	-	1114166..1114453 (+)	288	WP_136444324.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
J8Y17_RS05770 (J8Y17_05770)	gerPF	1114490..1114705 (-)	216	WP_001141566.1	spore germination protein GerPF	-
J8Y17_RS05775 (J8Y17_05775)	-	1114748..1115134 (-)	387	WP_000902315.1	spore germination protein GerPE	-
J8Y17_RS05780 (J8Y17_05780)	gerPD	1115150..1115344 (-)	195	WP_001102340.1	spore germination protein GerPD	-
J8Y17_RS05785 (J8Y17_05785)	gerPC	1115351..1115965 (-)	615	WP_001070766.1	spore germination protein GerPC	-
J8Y17_RS05790 (J8Y17_05790)	gerPB	1116033..1116239 (-)	207	WP_001012512.1	spore germination protein GerPB	-
J8Y17_RS05795 (J8Y17_05795)	gerPA	1116255..1116476 (-)	222	WP_001111188.1	spore germination protein GerPA	-
J8Y17_RS05800 (J8Y17_05800)	-	1116573..1116752 (-)	180	WP_000462851.1	aspartyl-phosphate phosphatase Spo0E family protein	-
J8Y17_RS05805 (J8Y17_05805)	-	1117000..1117899 (+)	900	WP_001241722.1	fumarylacetoacetate hydrolase family protein	-
J8Y17_RS05810 (J8Y17_05810)	-	1117935..1118219 (-)	285	WP_000925336.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142692.97 Da
• Isoelectric Point: 4.8199

>NTDB_id=555445 J8Y17_RS05760 WP_193108998.1 1110425..1114150(+) (addA) [Bacillus cereus strain BC07]
MMENWPKKPEGSQWTDDQWKAVVAKGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYDRLMWKYGYGEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIQTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEILQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGESGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=555445 J8Y17_RS05760 WP_193108998.1 1110425..1114150(+) (addA) [Bacillus cereus strain BC07]
ATGATGGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAAGGGGCG
TGATATTTTAGTCGCAGCAGCAGCAGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCAGCGCAAGAGATGAAAAACAGAATT
GGAGAGGCTTTAGAAAAAGTATTAATTGATGAACCAGGATCTCAGCACGTAAGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTCATTAGAGGATATTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACAGAAAATGAATTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATAGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAACGTATGATTTTAGC
GCTTCATACGGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTCGAAGGAA
AGACAATTGAAGATTTAGTGTACGCCTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCAACGGAGCTCGCAATGCTTCCTGACGGTCCGGCGCCTCGCGTTGAAACGCTGCAAGCAGATTTAGCTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCAATGCAAAACGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGCGATTATAACGAGGATATTGTCAAACAAGTAGACTCTCTTCGTAATAAAGCAAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCCGGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTCGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGCATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAGCAAAGTGAAGATGGTGAAATGAAGCCGTCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGTTTCACACAAGAAGGATTAGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACAAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCAGCTGAACTATTGTGCA
TTCAGCAAACAGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAAGCACAGCTTGAAGCACGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGACTTCGTTATTTTGCTCCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAGCTGCAAGGAATTCCAG
TATACGCTGACCTTGCCACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCAATGCAAGATATTCCGCTTGCAGCAGTGCTTCGCTCCCCAATCGTTGGATTAAATGATGAAGAGCTTGCAACGCTTCG
TGCTCACGGGAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAATGGTTCTACAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCGGTCGTATTCGTAGCTGGACTAGGTCGCCGCTTTAATACACAAGACTTAATGAA
GCGTTTCTTACTGCATAAAGACTTCGGTTTTGGTTCGCAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCGATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGTGTATTATACGTAGCGTTAACACGG
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAAATAAAGAAATGGAAAAGTGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCTGGAGCGTCTTGTTATTTAGACTGGATTGCCCCTTCCTTATATAGAC
ATCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGCATTCCAGATGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTTGATGGTAACACTTTACTTGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAGGCTGTTCCGTTAGAGAGTGAACGGAAAGAAGAAGTGTACGATAGATTAATGTGGAAGTACGGATATGGAG
AAGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCT
TTTATTAAAAAATTACGTGCACCAATTCAAACACGTCCTCGTTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGAGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGTTGAAATTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAAGAATTATTAACATTTGAACAAGCAGAAGAAATAGCAATTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGGAGAGCGGGGAATCAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATCACTTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCAGGGGGATTTGATCAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAGAAAAGTTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTATAAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1232)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.747	100	0.537