Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   J8Y17_RS00530 Genome accession   NZ_CP072769
Coordinates   97515..98891 (+) Length   458 a.a.
NCBI ID   WP_001085207.1    Uniprot ID   A0A2B4FB19
Organism   Bacillus cereus strain BC07     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 92515..103891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J8Y17_RS00510 (J8Y17_00510) ctsR 92722..93183 (+) 462 WP_001244563.1 transcriptional regulator CtsR -
  J8Y17_RS00515 (J8Y17_00515) - 93343..93891 (+) 549 WP_000128391.1 UvrB/UvrC motif-containing protein -
  J8Y17_RS00520 (J8Y17_00520) - 93896..94960 (+) 1065 WP_000050831.1 protein arginine kinase -
  J8Y17_RS00525 (J8Y17_00525) clpC 94983..97418 (+) 2436 WP_071730792.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  J8Y17_RS00530 (J8Y17_00530) radA 97515..98891 (+) 1377 WP_001085207.1 DNA repair protein RadA Machinery gene
  J8Y17_RS00535 (J8Y17_00535) disA 98895..99968 (+) 1074 WP_000392168.1 DNA integrity scanning diadenylate cyclase DisA -
  J8Y17_RS00540 (J8Y17_00540) - 100130..101239 (+) 1110 WP_000919684.1 PIN/TRAM domain-containing protein -
  J8Y17_RS00545 (J8Y17_00545) ispD 101256..101936 (+) 681 WP_088313184.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  J8Y17_RS00550 (J8Y17_00550) ispF 102051..102527 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49814.39 Da        Isoelectric Point: 7.1421

>NTDB_id=555437 J8Y17_RS00530 WP_001085207.1 97515..98891(+) (radA) [Bacillus cereus strain BC07]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADSSYDVLYISGEESAKQIKLRADRLHVNGSNLFVVSETDLQRIA
AHIEEVNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=555437 J8Y17_RS00530 WP_001085207.1 97515..98891(+) (radA) [Bacillus cereus strain BC07]
ATGGCTAAAAAGAAAACAAAATTCACATGTCAAGAGTGTGGGTATCAGTCACCGAAATATATGGGGAAATGCCCTGGATG
TGGTCAATGGAATACGCTTGTTGAAGAGATGGAACCGGTTGTATCATCAAGGCGCCTTAATTATGCGAATGCAATTCAAT
CGGAAGTAACAAAACCAAGACGCCTAACAGAAGTAGAAACAAAGTCTGAGGCGCGTATTGAAACGAAATTTCAAGAATTT
AATCGTGTACTTGGTGGCGGGATTGTAGATGGTTCTTTAGTACTTATTGGTGGAGACCCTGGGATTGGAAAATCAACGTT
ACTATTACAAATATCATCGCAATTAGCGGATTCTTCATATGATGTACTATATATATCGGGTGAAGAATCCGCAAAACAGA
TTAAACTGCGTGCGGATCGTTTACATGTAAATGGTAGTAATCTATTTGTTGTGTCAGAGACGGATTTACAGCGAATTGCG
GCACACATTGAAGAAGTGAATCCAGCCTTTGTTGTTATTGACTCTATTCAAACAATACATCTACCTGAAGTAACATCAGC
GCCGGGAAGTGTGGCACAAGTTCGTGAATGTACAGCTGAATTAATGAAACTTGCAAAAACGAAAGGAATCCCTATTTTTA
TCGTTGGACATGTGACAAAAGAGGGAGCAATTGCAGGACCTCGTATGTTAGAACATATGGTCGATGCAGTTCTTTATTTT
GAAGGAGACCGTCATCATACATACCGTATTTTGCGAGCAGTAAAGAATCGCTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAACTTGGTCTTGCAGAAGTATTAAACCCTTCTGAGATTTTCCTTGAGGAAAGACCAGTTGGGGTTGCGG
GATCAACAGTGGTTGCTTCAATGGAAGGAACAAGACCAGTTTTAGTAGAAATACAAGCTTTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCGGTGTTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGACGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCTATTGATTTAGCTG
TCGCTTTAAGTATAGCTTCAAGTTTCAGAGATAAATCTACAGCACCAACCGATGCAGTAATAGGAGAAGTAGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGTGCTATTATTCC
TAGAAAAAATTTGGGAGGATGGACAATTCCGGATGGGATTGAGGTAGTTGGTGTATCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(60-222)

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2B4FB19

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.728

100

0.749
  radA Streptococcus pneumoniae Rx1

63.797

98.908

0.631
  radA Streptococcus pneumoniae D39

63.797

98.908

0.631
  radA Streptococcus pneumoniae R6

63.797

98.908

0.631
  radA Streptococcus pneumoniae TIGR4

63.797

98.908

0.631
  radA Streptococcus mitis SK321

63.797

98.908

0.631
  radA Streptococcus mitis NCTC 12261

63.576

98.908

0.629