Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   L0024_RS01915 Genome accession   NZ_CP090647
Coordinates   416991..418355 (+) Length   454 a.a.
NCBI ID   WP_009875719.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PA1681     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 411991..423355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0024_RS01895 (L0024_01895) - 412829..413395 (-) 567 WP_003117889.1 hypothetical protein -
  L0024_RS01900 (L0024_01900) rsmD 413398..413994 (-) 597 WP_003102864.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  L0024_RS01905 (L0024_01905) - 413994..415481 (-) 1488 WP_003102862.1 pitrilysin family protein -
  L0024_RS01910 (L0024_01910) - 415474..416871 (-) 1398 WP_003084498.1 pitrilysin family protein -
  L0024_RS01915 (L0024_01915) pilA 416991..418355 (+) 1365 WP_009875719.1 signal recognition particle-docking protein FtsY Machinery gene
  L0024_RS01920 (L0024_01920) ftsE 418352..419023 (+) 672 WP_003084505.1 cell division ATP-binding protein FtsE -
  L0024_RS01925 (L0024_01925) ftsX 419023..420030 (+) 1008 WP_003084508.1 permease-like cell division protein FtsX -
  L0024_RS01930 (L0024_01930) rpoH 420144..420998 (+) 855 WP_003084510.1 RNA polymerase sigma factor RpoH -
  L0024_RS01935 (L0024_01935) - 421063..421668 (+) 606 WP_034017089.1 hypothetical protein -
  L0024_RS01940 (L0024_01940) mtgA 421673..422404 (-) 732 WP_003100972.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  L0024_RS01945 (L0024_01945) - 422441..422818 (+) 378 WP_003084513.1 DUF423 domain-containing protein -
  L0024_RS01950 (L0024_01950) thiS 422921..423121 (+) 201 WP_003084514.1 sulfur carrier protein ThiS -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 48324.73 Da        Isoelectric Point: 4.5272

>NTDB_id=555147 L0024_RS01915 WP_009875719.1 416991..418355(+) (pilA) [Pseudomonas aeruginosa strain PA1681]
MFGSNDDKKAPQGGEKKGLFGWWRKKPQAGEQPADQPVEPVSETAAAEQRAPADDVAQSLTEQPGRQQPSAAEPAEPAPV
AEAPLASDEPASAEEHSPRPEAPVAQPEPILAAEPEPEPEPEPEPEPEPVAPLAAAPAVSEPATRPGFFARLRQGLSKTS
ASIGEGMASLFLGRKEIDDDLLDDIETRLLTADVGVEATTLIVQNLTKRVARKELADSGALYKALQEELASLLRPVEQPL
QVDVAREPYVILVVGVNGVGKTTTIGKLAKKLQLEGKKVMLAAGDTFRAAAVEQLQVWGERNRIPVIAQHTGADSASVIF
DAVQAAKARGIDVLIADTAGRLHTKDNLMEELKKVRRVIGKLDETAPHEVLLVLDAGTGQNAINQAKQFNLAVELTGLAL
TKLDGTAKGGVIFALAKQFGLPIRYIGVGEGIDDLRTFEADAFVQALFAERENA

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=555147 L0024_RS01915 WP_009875719.1 416991..418355(+) (pilA) [Pseudomonas aeruginosa strain PA1681]
ATGTTTGGTTCCAACGACGACAAGAAGGCCCCGCAGGGCGGGGAAAAGAAAGGTCTGTTCGGCTGGTGGCGGAAAAAGCC
GCAAGCCGGCGAGCAGCCTGCCGATCAGCCTGTCGAGCCGGTCTCCGAGACAGCGGCCGCCGAGCAGCGCGCGCCTGCCG
ATGACGTGGCGCAATCCCTGACAGAACAGCCAGGTCGGCAGCAGCCCTCCGCTGCCGAGCCGGCCGAGCCGGCGCCGGTT
GCCGAAGCACCGCTCGCCAGTGACGAGCCAGCGTCGGCCGAGGAGCATTCGCCCCGGCCCGAGGCGCCCGTCGCGCAACC
CGAGCCTATCCTGGCTGCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGAGCCCGTTGCGCCTCTGG
CCGCCGCTCCGGCAGTCAGCGAGCCGGCGACCAGGCCGGGCTTCTTCGCCCGCCTCCGGCAGGGACTGTCCAAGACCAGC
GCCAGCATCGGCGAAGGCATGGCCAGCCTGTTCCTCGGCCGCAAGGAAATCGACGACGACCTGCTCGACGACATCGAGAC
GCGCCTGCTGACCGCCGACGTCGGCGTCGAGGCCACCACCCTGATCGTGCAGAACCTGACCAAGCGCGTGGCGCGCAAGG
AGCTGGCCGACAGCGGCGCGCTGTACAAGGCGCTGCAGGAAGAGCTGGCCAGCCTGCTGCGCCCGGTGGAGCAGCCGTTG
CAGGTCGACGTCGCGCGCGAGCCCTATGTGATCCTGGTGGTCGGGGTGAACGGCGTGGGCAAGACCACCACCATCGGCAA
GCTGGCGAAGAAGCTGCAACTCGAGGGCAAGAAGGTCATGCTGGCCGCCGGCGACACCTTCCGCGCCGCCGCCGTGGAGC
AGTTGCAGGTCTGGGGCGAGCGCAACCGCATCCCGGTGATCGCCCAGCACACCGGCGCCGACTCCGCCTCGGTGATCTTC
GACGCGGTGCAGGCCGCCAAGGCCCGCGGCATCGACGTGCTGATCGCCGATACCGCCGGACGCCTGCATACCAAGGACAA
CCTGATGGAGGAGCTGAAGAAGGTGCGCCGGGTGATCGGCAAGCTGGACGAGACGGCGCCCCATGAGGTCCTGCTGGTGC
TCGACGCCGGCACCGGGCAGAACGCCATCAACCAGGCCAAGCAGTTCAACCTCGCGGTGGAGCTGACCGGCCTGGCCCTG
ACCAAACTGGACGGCACCGCCAAGGGCGGGGTGATCTTCGCCCTGGCCAAGCAGTTCGGCCTGCCGATCCGCTATATCGG
TGTCGGCGAGGGGATCGACGATCTCCGTACCTTCGAGGCCGACGCCTTCGTCCAGGCACTCTTCGCAGAGCGGGAGAACG
CATGA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(164-229)

SRP54-type protein, GTPase domain (SRP54)

(249-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

47.018

96.035

0.452